Uncinula necator (Grape powdery mildew)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Erysiphe

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B1PC04|A0A0B1PC04_UNCNE Putative rna binding protein OS=Uncinula necator OX=52586 GN=EV44_g5223 PE=4 SV=1
MM1 pKa = 7.44SGAATEE7 pKa = 3.98TGYY10 pKa = 11.31DD11 pKa = 3.35VVVDD15 pKa = 3.69VDD17 pKa = 3.45EE18 pKa = 6.42DD19 pKa = 4.15GDD21 pKa = 4.32LGHH24 pKa = 7.38TDD26 pKa = 3.4LQEE29 pKa = 4.41DD30 pKa = 4.7LEE32 pKa = 4.49FHH34 pKa = 6.94SSNFNNFTSTTNTRR48 pKa = 11.84KK49 pKa = 9.7QPGAGLPPPATADD62 pKa = 3.67TTSKK66 pKa = 11.03KK67 pKa = 10.58YY68 pKa = 9.71MIWSLPFYY76 pKa = 10.89AQFFDD81 pKa = 3.45VDD83 pKa = 3.79TAAVLSRR90 pKa = 11.84CWATIYY96 pKa = 10.36PRR98 pKa = 11.84ANFTEE103 pKa = 4.21VMEE106 pKa = 4.82GNPDD110 pKa = 3.26LYY112 pKa = 11.26GPLWIATTVVFILFLGGTINRR133 pKa = 11.84YY134 pKa = 9.29LSEE137 pKa = 4.34TNGTQFVYY145 pKa = 10.83DD146 pKa = 4.03FTLLSGQQ153 pKa = 3.57

Molecular weight: 16.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B1P0A3|A0A0B1P0A3_UNCNE Putative replication factor c subunit 4 OS=Uncinula necator OX=52586 GN=EV44_g0959 PE=3 SV=1
MM1 pKa = 7.68NINSTVIKK9 pKa = 10.58KK10 pKa = 10.21NINSTRR16 pKa = 11.84FKK18 pKa = 10.94KK19 pKa = 10.62SINSTSFKK27 pKa = 11.08KK28 pKa = 10.61NINSSGIKK36 pKa = 10.19KK37 pKa = 10.24NINSTGNKK45 pKa = 8.85KK46 pKa = 10.61DD47 pKa = 3.67NNSTRR52 pKa = 11.84FKK54 pKa = 10.95KK55 pKa = 10.61SINSTSFKK63 pKa = 11.08KK64 pKa = 10.64NINSSGFKK72 pKa = 10.37KK73 pKa = 10.3IINSTGIKK81 pKa = 10.25KK82 pKa = 10.33NINSTRR88 pKa = 11.84FKK90 pKa = 11.22KK91 pKa = 10.62NINSTGFKK99 pKa = 10.64KK100 pKa = 10.63NINSTGFKK108 pKa = 10.63KK109 pKa = 10.62NXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIQRR163 pKa = 11.84ALRR166 pKa = 11.84RR167 pKa = 11.84TLIQRR172 pKa = 11.84ALRR175 pKa = 11.84RR176 pKa = 11.84TLIQRR181 pKa = 11.84ALRR184 pKa = 11.84RR185 pKa = 11.84TLIQRR190 pKa = 11.84ALRR193 pKa = 11.84RR194 pKa = 11.84TLIQRR199 pKa = 11.84ALRR202 pKa = 11.84RR203 pKa = 11.84ILIQRR208 pKa = 11.84DD209 pKa = 3.4LRR211 pKa = 11.84STLIQRR217 pKa = 11.84ALRR220 pKa = 11.84RR221 pKa = 11.84ALIQRR226 pKa = 11.84SLL228 pKa = 3.39

Molecular weight: 20.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6482

0

6482

3060911

66

4850

472.2

53.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.025

1.353 ± 0.012

5.324 ± 0.02

6.527 ± 0.028

3.892 ± 0.015

5.393 ± 0.028

2.211 ± 0.012

6.706 ± 0.021

6.606 ± 0.029

9.179 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.01

5.319 ± 0.02

5.054 ± 0.027

4.109 ± 0.02

5.627 ± 0.022

9.324 ± 0.034

5.724 ± 0.017

5.257 ± 0.018

1.194 ± 0.009

2.796 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski