Sphingomonas sp. Root710

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8QIU3|A0A0Q8QIU3_9SPHN 30S ribosomal protein S7 OS=Sphingomonas sp. Root710 OX=1736594 GN=rpsG PE=3 SV=1
MM1 pKa = 7.57ASINSVQDD9 pKa = 3.28QEE11 pKa = 4.45VTFISGVDD19 pKa = 3.42SSGRR23 pKa = 11.84AAPISFGTWNYY34 pKa = 10.81DD35 pKa = 3.16SPATYY40 pKa = 10.34DD41 pKa = 3.45GSAGNNYY48 pKa = 7.09TAKK51 pKa = 10.46FGPATSGTGATITYY65 pKa = 10.31AFDD68 pKa = 3.39IASNWTATEE77 pKa = 3.64KK78 pKa = 10.95AAFVATAKK86 pKa = 10.12LWSAVADD93 pKa = 3.72VTFVEE98 pKa = 5.19ASPSSAQVMLTRR110 pKa = 11.84STDD113 pKa = 3.15EE114 pKa = 4.3SASGGPDD121 pKa = 2.88RR122 pKa = 11.84FTTATTGTNQLGVASTASINIDD144 pKa = 3.26TTVAAFGPLGTSLSNYY160 pKa = 9.25GGYY163 pKa = 9.88PYY165 pKa = 7.97TTLIHH170 pKa = 6.04EE171 pKa = 4.6WGHH174 pKa = 5.02VLGLGHH180 pKa = 7.24GGPYY184 pKa = 10.0NAGDD188 pKa = 3.73VDD190 pKa = 3.84EE191 pKa = 4.7TPYY194 pKa = 10.89TGFDD198 pKa = 3.21NVAWTIMSYY207 pKa = 11.13NDD209 pKa = 3.68QSAEE213 pKa = 3.93WGVSRR218 pKa = 11.84GDD220 pKa = 3.57DD221 pKa = 3.28GLFYY225 pKa = 10.96GRR227 pKa = 11.84SPTTWMPLDD236 pKa = 4.41IIAAQRR242 pKa = 11.84LYY244 pKa = 10.8GVAVNTPLSGGQVYY258 pKa = 9.83GFNSNITGEE267 pKa = 3.91IAKK270 pKa = 10.19FFDD273 pKa = 4.27FNQNTRR279 pKa = 11.84PIVTLWNKK287 pKa = 9.28GVNNTLDD294 pKa = 3.39LSGTSFASTVDD305 pKa = 3.19MHH307 pKa = 7.29DD308 pKa = 3.58GAFSSVMNLEE318 pKa = 4.59DD319 pKa = 3.93NLAIAYY325 pKa = 6.76GTRR328 pKa = 11.84IDD330 pKa = 3.94TVITGAGGDD339 pKa = 3.65MVTGNDD345 pKa = 3.04NSNYY349 pKa = 9.44VLAGAGADD357 pKa = 3.89SIIGGSGNDD366 pKa = 3.68HH367 pKa = 7.19LYY369 pKa = 10.6GAAAVAVAGDD379 pKa = 3.76GADD382 pKa = 3.87TIGGGAGSDD391 pKa = 3.89YY392 pKa = 11.01IQGNAGDD399 pKa = 4.31DD400 pKa = 3.66RR401 pKa = 11.84LNGGDD406 pKa = 3.64GSDD409 pKa = 4.61RR410 pKa = 11.84IQGGQGNDD418 pKa = 3.87SILGDD423 pKa = 4.04AGNDD427 pKa = 3.54TTNGNLGNDD436 pKa = 4.12SIDD439 pKa = 3.48GGEE442 pKa = 4.5GNDD445 pKa = 4.02SLRR448 pKa = 11.84GGQGADD454 pKa = 3.79SITGGIGNDD463 pKa = 3.43MLLGDD468 pKa = 5.13LGVDD472 pKa = 3.71TLSGGAGIDD481 pKa = 3.75LMSGGGDD488 pKa = 3.22PDD490 pKa = 2.94IFTFAEE496 pKa = 4.43GDD498 pKa = 3.27ASFGNGSATDD508 pKa = 3.99VITDD512 pKa = 4.02FTDD515 pKa = 4.37EE516 pKa = 4.06IDD518 pKa = 4.6HH519 pKa = 6.22IHH521 pKa = 6.02MAFGLPSAVLHH532 pKa = 6.06GAAADD537 pKa = 3.93SFAQAAASAQQILNGQAGFTDD558 pKa = 3.8VAVLQVGTDD567 pKa = 3.08VYY569 pKa = 11.39LFYY572 pKa = 10.37DD573 pKa = 4.07TGAAAPLEE581 pKa = 4.47AIRR584 pKa = 11.84LGGIADD590 pKa = 3.8AAVITITDD598 pKa = 4.05FTT600 pKa = 4.45

Molecular weight: 60.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8QBP1|A0A0Q8QBP1_9SPHN RES domain-containing protein OS=Sphingomonas sp. Root710 OX=1736594 GN=ASE00_20535 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 4.0

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4422

0

4422

1395197

29

1722

315.5

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.257 ± 0.051

0.796 ± 0.01

6.084 ± 0.029

5.308 ± 0.032

3.56 ± 0.021

8.923 ± 0.035

2.052 ± 0.018

5.39 ± 0.022

3.023 ± 0.027

9.845 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.017

2.532 ± 0.025

5.253 ± 0.028

3.016 ± 0.019

7.419 ± 0.038

5.348 ± 0.029

5.058 ± 0.03

6.96 ± 0.03

1.384 ± 0.014

2.332 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski