Amino acid dipepetide frequency for Sphingomonas sp. Root710

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.804AlaAla: 19.804 ± 0.197	1.018AlaCys: 1.018 ± 0.031	7.781AlaAsp: 7.781 ± 0.084	7.776AlaGlu: 7.776 ± 0.096	4.322AlaPhe: 4.322 ± 0.059	11.576AlaGly: 11.576 ± 0.1	2.361AlaHis: 2.361 ± 0.038	7.163AlaIle: 7.163 ± 0.086	4.127AlaLys: 4.127 ± 0.067	13.582AlaLeu: 13.582 ± 0.144	3.893AlaMet: 3.893 ± 0.061	3.081AlaAsn: 3.081 ± 0.053	6.188AlaPro: 6.188 ± 0.084	4.28AlaGln: 4.28 ± 0.058	9.777AlaArg: 9.777 ± 0.087	6.574AlaSer: 6.574 ± 0.077	6.31AlaThr: 6.31 ± 0.076	8.605AlaVal: 8.605 ± 0.092	1.62AlaTrp: 1.62 ± 0.034	2.732AlaTyr: 2.732 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.967CysAla: 0.967 ± 0.03	0.099CysCys: 0.099 ± 0.008	0.576CysAsp: 0.576 ± 0.021	0.388CysGlu: 0.388 ± 0.017	0.343CysPhe: 0.343 ± 0.016	0.884CysGly: 0.884 ± 0.028	0.232CysHis: 0.232 ± 0.015	0.394CysIle: 0.394 ± 0.017	0.163CysLys: 0.163 ± 0.012	0.703CysLeu: 0.703 ± 0.023	0.169CysMet: 0.169 ± 0.014	0.205CysAsn: 0.205 ± 0.013	0.409CysPro: 0.409 ± 0.018	0.194CysGln: 0.194 ± 0.012	0.571CysArg: 0.571 ± 0.023	0.447CysSer: 0.447 ± 0.017	0.397CysThr: 0.397 ± 0.015	0.537CysVal: 0.537 ± 0.018	0.104CysTrp: 0.104 ± 0.008	0.181CysTyr: 0.181 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.738AspAla: 7.738 ± 0.083	0.456AspCys: 0.456 ± 0.018	3.464AspAsp: 3.464 ± 0.055	3.386AspGlu: 3.386 ± 0.053	2.17AspPhe: 2.17 ± 0.041	5.733AspGly: 5.733 ± 0.074	1.449AspHis: 1.449 ± 0.033	3.372AspIle: 3.372 ± 0.058	1.76AspLys: 1.76 ± 0.037	5.875AspLeu: 5.875 ± 0.074	1.476AspMet: 1.476 ± 0.035	1.377AspAsn: 1.377 ± 0.03	3.829AspPro: 3.829 ± 0.059	1.9AspGln: 1.9 ± 0.038	5.082AspArg: 5.082 ± 0.067	2.461AspSer: 2.461 ± 0.04	2.309AspThr: 2.309 ± 0.048	4.155AspVal: 4.155 ± 0.065	1.069AspTrp: 1.069 ± 0.027	1.78AspTyr: 1.78 ± 0.035	0.001AspXaa: 0.001 ± 0.001
Glu	7.775GluAla: 7.775 ± 0.09	0.307GluCys: 0.307 ± 0.015	2.67GluAsp: 2.67 ± 0.05	2.842GluGlu: 2.842 ± 0.058	1.399GluPhe: 1.399 ± 0.031	4.306GluGly: 4.306 ± 0.068	1.176GluHis: 1.176 ± 0.034	3.058GluIle: 3.058 ± 0.052	1.92GluLys: 1.92 ± 0.04	5.105GluLeu: 5.105 ± 0.069	1.356GluMet: 1.356 ± 0.036	1.289GluAsn: 1.289 ± 0.032	2.518GluPro: 2.518 ± 0.047	2.061GluGln: 2.061 ± 0.041	4.888GluArg: 4.888 ± 0.076	2.233GluSer: 2.233 ± 0.039	3.068GluThr: 3.068 ± 0.051	3.423GluVal: 3.423 ± 0.048	0.69GluTrp: 0.69 ± 0.022	0.999GluTyr: 0.999 ± 0.026	0.001GluXaa: 0.001 ± 0.001
Phe	4.722PheAla: 4.722 ± 0.059	0.323PheCys: 0.323 ± 0.016	2.792PheAsp: 2.792 ± 0.046	2.051PheGlu: 2.051 ± 0.035	1.287PhePhe: 1.287 ± 0.04	3.671PheGly: 3.671 ± 0.056	0.795PheHis: 0.795 ± 0.024	1.527PheIle: 1.527 ± 0.038	0.905PheLys: 0.905 ± 0.026	3.091PheLeu: 3.091 ± 0.063	0.728PheMet: 0.728 ± 0.023	1.055PheAsn: 1.055 ± 0.031	1.528PhePro: 1.528 ± 0.034	0.93PheGln: 0.93 ± 0.025	2.242PheArg: 2.242 ± 0.035	2.031PheSer: 2.031 ± 0.042	2.007PheThr: 2.007 ± 0.044	2.516PheVal: 2.516 ± 0.045	0.485PheTrp: 0.485 ± 0.021	0.912PheTyr: 0.912 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	9.926GlyAla: 9.926 ± 0.09	0.834GlyCys: 0.834 ± 0.026	5.153GlyAsp: 5.153 ± 0.067	4.64GlyGlu: 4.64 ± 0.059	3.644GlyPhe: 3.644 ± 0.056	8.411GlyGly: 8.411 ± 0.131	1.952GlyHis: 1.952 ± 0.038	4.61GlyIle: 4.61 ± 0.061	3.232GlyLys: 3.232 ± 0.054	8.807GlyLeu: 8.807 ± 0.083	2.344GlyMet: 2.344 ± 0.054	2.296GlyAsn: 2.296 ± 0.056	3.797GlyPro: 3.797 ± 0.055	2.975GlyGln: 2.975 ± 0.052	6.742GlyArg: 6.742 ± 0.075	4.912GlySer: 4.912 ± 0.081	4.594GlyThr: 4.594 ± 0.069	6.258GlyVal: 6.258 ± 0.082	1.627GlyTrp: 1.627 ± 0.034	2.47GlyTyr: 2.47 ± 0.045	0.001GlyXaa: 0.001 ± 0.001
His	2.436HisAla: 2.436 ± 0.043	0.242HisCys: 0.242 ± 0.015	1.275HisAsp: 1.275 ± 0.03	0.949HisGlu: 0.949 ± 0.026	0.881HisPhe: 0.881 ± 0.026	2.074HisGly: 2.074 ± 0.036	0.615HisHis: 0.615 ± 0.022	1.067HisIle: 1.067 ± 0.028	0.491HisLys: 0.491 ± 0.017	1.938HisLeu: 1.938 ± 0.036	0.528HisMet: 0.528 ± 0.021	0.474HisAsn: 0.474 ± 0.017	1.329HisPro: 1.329 ± 0.034	0.599HisGln: 0.599 ± 0.021	1.568HisArg: 1.568 ± 0.041	0.997HisSer: 0.997 ± 0.026	0.591HisThr: 0.591 ± 0.019	1.511HisVal: 1.511 ± 0.034	0.361HisTrp: 0.361 ± 0.014	0.596HisTyr: 0.596 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.235IleAla: 8.235 ± 0.087	0.49IleCys: 0.49 ± 0.019	4.395IleAsp: 4.395 ± 0.055	3.815IleGlu: 3.815 ± 0.063	1.793IlePhe: 1.793 ± 0.035	5.503IleGly: 5.503 ± 0.07	0.983IleHis: 0.983 ± 0.026	2.312IleIle: 2.312 ± 0.046	1.256IleLys: 1.256 ± 0.03	4.234IleLeu: 4.234 ± 0.066	0.955IleMet: 0.955 ± 0.027	1.388IleAsn: 1.388 ± 0.033	2.269IlePro: 2.269 ± 0.038	1.252IleGln: 1.252 ± 0.031	3.443IleArg: 3.443 ± 0.046	2.991IleSer: 2.991 ± 0.049	2.643IleThr: 2.643 ± 0.045	4.235IleVal: 4.235 ± 0.061	0.624IleTrp: 0.624 ± 0.023	1.085IleTyr: 1.085 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.277LysAla: 4.277 ± 0.068	0.144LysCys: 0.144 ± 0.01	1.486LysAsp: 1.486 ± 0.034	1.159LysGlu: 1.159 ± 0.033	0.84LysPhe: 0.84 ± 0.027	2.659LysGly: 2.659 ± 0.046	0.553LysHis: 0.553 ± 0.02	1.651LysIle: 1.651 ± 0.039	1.068LysLys: 1.068 ± 0.035	3.329LysLeu: 3.329 ± 0.052	0.783LysMet: 0.783 ± 0.022	0.733LysAsn: 0.733 ± 0.025	2.022LysPro: 2.022 ± 0.039	0.93LysGln: 0.93 ± 0.027	2.237LysArg: 2.237 ± 0.045	1.526LysSer: 1.526 ± 0.033	1.654LysThr: 1.654 ± 0.041	2.184LysVal: 2.184 ± 0.043	0.371LysTrp: 0.371 ± 0.016	0.63LysTyr: 0.63 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	13.608LeuAla: 13.608 ± 0.133	0.842LeuCys: 0.842 ± 0.024	6.363LeuAsp: 6.363 ± 0.069	4.574LeuGlu: 4.574 ± 0.059	3.71LeuPhe: 3.71 ± 0.059	8.361LeuGly: 8.361 ± 0.088	1.817LeuHis: 1.817 ± 0.035	5.403LeuIle: 5.403 ± 0.081	3.057LeuLys: 3.057 ± 0.056	9.634LeuLeu: 9.634 ± 0.112	2.117LeuMet: 2.117 ± 0.049	2.467LeuAsn: 2.467 ± 0.047	5.355LeuPro: 5.355 ± 0.065	2.445LeuGln: 2.445 ± 0.044	6.882LeuArg: 6.882 ± 0.082	6.281LeuSer: 6.281 ± 0.073	5.482LeuThr: 5.482 ± 0.064	6.725LeuVal: 6.725 ± 0.075	1.264LeuTrp: 1.264 ± 0.037	2.06LeuTyr: 2.06 ± 0.038	0.004LeuXaa: 0.004 ± 0.002
Met	3.321MetAla: 3.321 ± 0.057	0.177MetCys: 0.177 ± 0.011	1.083MetAsp: 1.083 ± 0.031	1.029MetGlu: 1.029 ± 0.033	0.701MetPhe: 0.701 ± 0.023	1.823MetGly: 1.823 ± 0.038	0.443MetHis: 0.443 ± 0.019	1.588MetIle: 1.588 ± 0.036	0.92MetLys: 0.92 ± 0.027	2.638MetLeu: 2.638 ± 0.047	0.7MetMet: 0.7 ± 0.025	0.657MetAsn: 0.657 ± 0.023	1.496MetPro: 1.496 ± 0.033	0.664MetGln: 0.664 ± 0.022	1.848MetArg: 1.848 ± 0.031	1.541MetSer: 1.541 ± 0.034	1.804MetThr: 1.804 ± 0.035	1.606MetVal: 1.606 ± 0.035	0.235MetTrp: 0.235 ± 0.015	0.3MetTyr: 0.3 ± 0.013	0.001MetXaa: 0.001 ± 0.001
Asn	3.209AsnAla: 3.209 ± 0.052	0.237AsnCys: 0.237 ± 0.012	1.438AsnAsp: 1.438 ± 0.035	1.112AsnGlu: 1.112 ± 0.031	0.908AsnPhe: 0.908 ± 0.029	2.385AsnGly: 2.385 ± 0.046	0.517AsnHis: 0.517 ± 0.018	1.487AsnIle: 1.487 ± 0.034	0.652AsnLys: 0.652 ± 0.023	2.452AsnLeu: 2.452 ± 0.044	0.616AsnMet: 0.616 ± 0.021	0.696AsnAsn: 0.696 ± 0.027	1.626AsnPro: 1.626 ± 0.028	0.715AsnGln: 0.715 ± 0.025	1.864AsnArg: 1.864 ± 0.032	1.349AsnSer: 1.349 ± 0.034	1.001AsnThr: 1.001 ± 0.024	1.92AsnVal: 1.92 ± 0.038	0.421AsnTrp: 0.421 ± 0.019	0.711AsnTyr: 0.711 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.12ProAla: 7.12 ± 0.094	0.317ProCys: 0.317 ± 0.014	3.703ProAsp: 3.703 ± 0.056	3.26ProGlu: 3.26 ± 0.057	1.923ProPhe: 1.923 ± 0.035	4.872ProGly: 4.872 ± 0.064	1.011ProHis: 1.011 ± 0.034	2.647ProIle: 2.647 ± 0.043	1.561ProLys: 1.561 ± 0.035	4.948ProLeu: 4.948 ± 0.06	1.199ProMet: 1.199 ± 0.03	1.277ProAsn: 1.277 ± 0.031	2.858ProPro: 2.858 ± 0.066	1.535ProGln: 1.535 ± 0.035	3.077ProArg: 3.077 ± 0.052	2.767ProSer: 2.767 ± 0.05	2.4ProThr: 2.4 ± 0.04	4.22ProVal: 4.22 ± 0.061	0.675ProTrp: 0.675 ± 0.02	1.161ProTyr: 1.161 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.134GlnAla: 4.134 ± 0.059	0.214GlnCys: 0.214 ± 0.011	1.396GlnAsp: 1.396 ± 0.03	1.256GlnGlu: 1.256 ± 0.032	1.074GlnPhe: 1.074 ± 0.031	2.473GlnGly: 2.473 ± 0.046	0.663GlnHis: 0.663 ± 0.023	1.707GlnIle: 1.707 ± 0.034	0.943GlnLys: 0.943 ± 0.029	3.14GlnLeu: 3.14 ± 0.043	0.783GlnMet: 0.783 ± 0.026	0.744GlnAsn: 0.744 ± 0.026	1.765GlnPro: 1.765 ± 0.039	1.067GlnGln: 1.067 ± 0.028	2.494GlnArg: 2.494 ± 0.047	1.659GlnSer: 1.659 ± 0.034	1.567GlnThr: 1.567 ± 0.034	2.043GlnVal: 2.043 ± 0.038	0.391GlnTrp: 0.391 ± 0.017	0.651GlnTyr: 0.651 ± 0.025	0.001GlnXaa: 0.001 ± 0.001
Arg	9.007ArgAla: 9.007 ± 0.092	0.558ArgCys: 0.558 ± 0.021	4.445ArgAsp: 4.445 ± 0.065	3.918ArgGlu: 3.918 ± 0.058	3.106ArgPhe: 3.106 ± 0.048	5.166ArgGly: 5.166 ± 0.068	1.776ArgHis: 1.776 ± 0.039	4.34ArgIle: 4.34 ± 0.057	2.136ArgLys: 2.136 ± 0.043	8.333ArgLeu: 8.333 ± 0.099	1.945ArgMet: 1.945 ± 0.037	1.932ArgAsn: 1.932 ± 0.036	3.804ArgPro: 3.804 ± 0.056	2.444ArgGln: 2.444 ± 0.043	6.034ArgArg: 6.034 ± 0.073	3.837ArgSer: 3.837 ± 0.051	3.56ArgThr: 3.56 ± 0.052	4.619ArgVal: 4.619 ± 0.057	1.225ArgTrp: 1.225 ± 0.031	2.006ArgTyr: 2.006 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.56SerAla: 6.56 ± 0.071	0.432SerCys: 0.432 ± 0.018	3.167SerAsp: 3.167 ± 0.05	2.495SerGlu: 2.495 ± 0.04	2.356SerPhe: 2.356 ± 0.043	5.502SerGly: 5.502 ± 0.079	0.977SerHis: 0.977 ± 0.027	2.912SerIle: 2.912 ± 0.049	1.52SerLys: 1.52 ± 0.036	5.205SerLeu: 5.205 ± 0.059	1.266SerMet: 1.266 ± 0.032	1.381SerAsn: 1.381 ± 0.032	2.926SerPro: 2.926 ± 0.045	1.471SerGln: 1.471 ± 0.032	3.652SerArg: 3.652 ± 0.058	2.987SerSer: 2.987 ± 0.056	2.575SerThr: 2.575 ± 0.046	3.715SerVal: 3.715 ± 0.057	0.815SerTrp: 0.815 ± 0.026	1.57SerTyr: 1.57 ± 0.032	0.001SerXaa: 0.001 ± 0.001
Thr	6.254ThrAla: 6.254 ± 0.072	0.35ThrCys: 0.35 ± 0.017	2.847ThrAsp: 2.847 ± 0.05	2.243ThrGlu: 2.243 ± 0.039	1.64ThrPhe: 1.64 ± 0.04	5.237ThrGly: 5.237 ± 0.077	0.935ThrHis: 0.935 ± 0.027	3.128ThrIle: 3.128 ± 0.049	1.313ThrLys: 1.313 ± 0.034	5.54ThrLeu: 5.54 ± 0.071	1.223ThrMet: 1.223 ± 0.033	1.199ThrAsn: 1.199 ± 0.032	3.303ThrPro: 3.303 ± 0.05	1.393ThrGln: 1.393 ± 0.034	3.278ThrArg: 3.278 ± 0.052	2.668ThrSer: 2.668 ± 0.049	2.566ThrThr: 2.566 ± 0.039	3.772ThrVal: 3.772 ± 0.055	0.53ThrTrp: 0.53 ± 0.019	1.164ThrTyr: 1.164 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	9.316ValAla: 9.316 ± 0.094	0.554ValCys: 0.554 ± 0.02	4.513ValAsp: 4.513 ± 0.059	4.422ValGlu: 4.422 ± 0.06	2.065ValPhe: 2.065 ± 0.036	5.429ValGly: 5.429 ± 0.061	1.38ValHis: 1.38 ± 0.033	3.854ValIle: 3.854 ± 0.052	2.066ValLys: 2.066 ± 0.038	6.023ValLeu: 6.023 ± 0.076	1.684ValMet: 1.684 ± 0.035	1.923ValAsn: 1.923 ± 0.039	3.756ValPro: 3.756 ± 0.051	1.995ValGln: 1.995 ± 0.043	5.196ValArg: 5.196 ± 0.062	4.06ValSer: 4.06 ± 0.055	4.173ValThr: 4.173 ± 0.063	5.085ValVal: 5.085 ± 0.065	0.768ValTrp: 0.768 ± 0.023	1.339ValTyr: 1.339 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.365TrpAla: 1.365 ± 0.032	0.114TrpCys: 0.114 ± 0.01	0.679TrpAsp: 0.679 ± 0.023	0.554TrpGlu: 0.554 ± 0.019	0.503TrpPhe: 0.503 ± 0.021	0.936TrpGly: 0.936 ± 0.025	0.347TrpHis: 0.347 ± 0.017	0.738TrpIle: 0.738 ± 0.022	0.489TrpLys: 0.489 ± 0.019	1.674TrpLeu: 1.674 ± 0.041	0.363TrpMet: 0.363 ± 0.017	0.472TrpAsn: 0.472 ± 0.017	0.712TrpPro: 0.712 ± 0.023	0.563TrpGln: 0.563 ± 0.02	1.323TrpArg: 1.323 ± 0.03	0.881TrpSer: 0.881 ± 0.024	0.839TrpThr: 0.839 ± 0.027	0.796TrpVal: 0.796 ± 0.025	0.204TrpTrp: 0.204 ± 0.014	0.282TrpTyr: 0.282 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.795TyrAla: 2.795 ± 0.048	0.254TyrCys: 0.254 ± 0.013	1.616TyrAsp: 1.616 ± 0.033	1.215TyrGlu: 1.215 ± 0.034	0.932TyrPhe: 0.932 ± 0.028	2.222TyrGly: 2.222 ± 0.043	0.54TyrHis: 0.54 ± 0.018	0.94TyrIle: 0.94 ± 0.026	0.655TyrLys: 0.655 ± 0.026	2.195TyrLeu: 2.195 ± 0.043	0.447TyrMet: 0.447 ± 0.019	0.669TyrAsn: 0.669 ± 0.024	1.04TyrPro: 1.04 ± 0.031	0.753TyrGln: 0.753 ± 0.023	1.993TyrArg: 1.993 ± 0.037	1.284TyrSer: 1.284 ± 0.031	1.044TyrThr: 1.044 ± 0.027	1.678TyrVal: 1.678 ± 0.039	0.358TyrTrp: 0.358 ± 0.015	0.692TyrTyr: 0.692 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4422 proteins (1395197 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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