Sesbania mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9EB09|Q9EB09_9VIRU Putative movement protein OS=Sesbania mosaic virus OX=12558 PE=4 SV=1
MM1 pKa = 7.06QAQHH5 pKa = 6.06TFTIKK10 pKa = 10.58FLRR13 pKa = 11.84HH14 pKa = 4.65ACFIGFEE21 pKa = 4.24DD22 pKa = 3.78PRR24 pKa = 11.84VVLDD28 pKa = 4.12HH29 pKa = 6.96EE30 pKa = 5.35EE31 pKa = 3.66IDD33 pKa = 3.71IPCEE37 pKa = 4.19VEE39 pKa = 3.77FDD41 pKa = 4.2CNAEE45 pKa = 4.35SVCLVRR51 pKa = 11.84AHH53 pKa = 5.79NQPYY57 pKa = 10.39SEE59 pKa = 4.8GEE61 pKa = 4.07VKK63 pKa = 10.66NYY65 pKa = 10.35SVYY68 pKa = 10.54FNSGLNDD75 pKa = 3.38YY76 pKa = 10.0YY77 pKa = 11.57GPLLPVWLEE86 pKa = 3.7IVCRR90 pKa = 11.84VCATSYY96 pKa = 11.25SFVLAPEE103 pKa = 4.45DD104 pKa = 4.28LEE106 pKa = 6.03DD107 pKa = 3.44EE108 pKa = 4.58AGRR111 pKa = 11.84VSRR114 pKa = 11.84KK115 pKa = 8.96YY116 pKa = 10.72APCEE120 pKa = 3.67NGKK123 pKa = 9.37VFQRR127 pKa = 11.84AVRR130 pKa = 11.84RR131 pKa = 11.84ATGEE135 pKa = 3.93EE136 pKa = 4.18YY137 pKa = 10.27FYY139 pKa = 11.48SCRR142 pKa = 11.84KK143 pKa = 8.72KK144 pKa = 10.39VCRR147 pKa = 11.84EE148 pKa = 3.93CIIRR152 pKa = 11.84AARR155 pKa = 11.84AMSSSS160 pKa = 3.21

Molecular weight: 18.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9EB07|Q9EB07_9VIRU RNA-directed RNA polymerase OS=Sesbania mosaic virus OX=12558 PE=4 SV=2
MM1 pKa = 7.88AKK3 pKa = 10.14RR4 pKa = 11.84LSKK7 pKa = 10.44QQLAKK12 pKa = 10.73AIANTLEE19 pKa = 4.36TPPQPKK25 pKa = 9.71AGRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84NRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84QRR36 pKa = 11.84SAVQQLQPTQAGISMAPSAQGAMVRR61 pKa = 11.84IRR63 pKa = 11.84NPAVSSSRR71 pKa = 11.84GGITVLTHH79 pKa = 6.31SEE81 pKa = 3.87LSAEE85 pKa = 4.11IGVTDD90 pKa = 4.41SIVVSSEE97 pKa = 3.28LVMPYY102 pKa = 9.63TVGTWLRR109 pKa = 11.84GVAANWSKK117 pKa = 11.18YY118 pKa = 7.01SWLSVRR124 pKa = 11.84YY125 pKa = 7.63TYY127 pKa = 10.52IPSCPSSTAGSIHH140 pKa = 6.68MGFQYY145 pKa = 11.56DD146 pKa = 3.5MADD149 pKa = 3.54TVPVSVNQLSNLRR162 pKa = 11.84GYY164 pKa = 11.09VSGQVWSGSAGLCFINGTRR183 pKa = 11.84CSDD186 pKa = 3.0TSTAISTTLDD196 pKa = 3.19VSKK199 pKa = 10.62LGKK202 pKa = 9.35KK203 pKa = 8.4WYY205 pKa = 8.96PYY207 pKa = 8.6KK208 pKa = 10.61TSADD212 pKa = 3.43YY213 pKa = 8.57ATAVGVDD220 pKa = 3.59VNIATPLVPARR231 pKa = 11.84LVIALLDD238 pKa = 4.31GSSSTAVAAGRR249 pKa = 11.84IYY251 pKa = 10.13CTYY254 pKa = 8.53TIQMIEE260 pKa = 4.11PTASALNNN268 pKa = 3.59

Molecular weight: 28.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1521

160

572

380.3

41.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.284 ± 0.654

3.287 ± 0.715

3.813 ± 0.356

6.377 ± 1.233

3.024 ± 0.771

6.443 ± 0.343

1.775 ± 0.267

3.813 ± 0.479

5.128 ± 0.633

9.007 ± 0.839

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.236

2.893 ± 0.297

6.772 ± 0.75

3.287 ± 0.371

5.588 ± 0.889

10.717 ± 0.942

5.194 ± 0.981

7.101 ± 0.678

1.972 ± 0.303

3.419 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski