Mycobacterium phage MrMiyagi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fowlmouthvirus; unclassified Fowlmouthvirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LPU7|A0A7G8LPU7_9CAUD Membrane protein OS=Mycobacterium phage MrMiyagi OX=2762395 GN=55 PE=4 SV=1
MM1 pKa = 7.1TAPIVLNVLGYY12 pKa = 8.76FSPSPKK18 pKa = 9.48YY19 pKa = 9.37ANSVAMVLDD28 pKa = 4.61LTDD31 pKa = 3.1SWDD34 pKa = 3.69DD35 pKa = 3.7DD36 pKa = 4.33FGDD39 pKa = 3.44VDD41 pKa = 3.75TYY43 pKa = 9.96GTQVMVFYY51 pKa = 10.96RR52 pKa = 11.84FTIQEE57 pKa = 3.67AYY59 pKa = 10.37AAVNAYY65 pKa = 8.84IQNTTTGFNSFVPWRR80 pKa = 11.84DD81 pKa = 2.74ASQLIDD87 pKa = 5.03IITEE91 pKa = 4.02GQNWIVTTDD100 pKa = 2.62SDD102 pKa = 4.04GFVNAEE108 pKa = 4.06CFPNLEE114 pKa = 4.05EE115 pKa = 5.09AEE117 pKa = 4.35RR118 pKa = 11.84IYY120 pKa = 11.1LDD122 pKa = 3.32RR123 pKa = 11.84RR124 pKa = 11.84TDD126 pKa = 3.77FQVWAKK132 pKa = 9.77EE133 pKa = 4.11RR134 pKa = 11.84PP135 pKa = 3.5

Molecular weight: 15.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LQ14|A0A7G8LQ14_9CAUD Uncharacterized protein OS=Mycobacterium phage MrMiyagi OX=2762395 GN=116 PE=4 SV=1
MM1 pKa = 7.64FKK3 pKa = 10.4RR4 pKa = 11.84IAGAIIIAVFSMALVIAPVSASPTYY29 pKa = 10.2IAGPEE34 pKa = 3.75PTPIIKK40 pKa = 10.02CRR42 pKa = 11.84NQSHH46 pKa = 5.29VWNPEE51 pKa = 3.48QCDD54 pKa = 3.27RR55 pKa = 11.84PAPFTIGGGGSGRR68 pKa = 11.84SNGGLLGRR76 pKa = 11.84IGGIIGRR83 pKa = 11.84LTGGILL89 pKa = 3.37

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

121

0

121

21892

40

1839

180.9

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.884 ± 0.434

0.9 ± 0.129

6.413 ± 0.212

6.564 ± 0.258

3.896 ± 0.153

7.765 ± 0.374

1.882 ± 0.154

5.838 ± 0.201

5.705 ± 0.326

7.711 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.187

4.536 ± 0.193

4.952 ± 0.156

4.075 ± 0.224

5.76 ± 0.333

6.733 ± 0.255

5.472 ± 0.24

6.491 ± 0.19

1.937 ± 0.134

2.955 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski