Amino acid dipepetide frequency for Mycobacterium phage MrMiyagi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.121AlaAla: 6.121 ± 1.332
0.457AlaCys: 0.457 ± 0.142
5.253AlaAsp: 5.253 ± 0.394
4.934AlaGlu: 4.934 ± 0.474
2.924AlaPhe: 2.924 ± 0.339
4.796AlaGly: 4.796 ± 0.562
1.69AlaHis: 1.69 ± 0.291
5.071AlaIle: 5.071 ± 0.504
5.664AlaLys: 5.664 ± 0.522
6.578AlaLeu: 6.578 ± 0.679
2.467AlaMet: 2.467 ± 0.4
3.7AlaAsn: 3.7 ± 0.601
2.558AlaPro: 2.558 ± 0.289
3.426AlaGln: 3.426 ± 0.633
4.431AlaArg: 4.431 ± 0.47
5.071AlaSer: 5.071 ± 0.495
4.568AlaThr: 4.568 ± 0.521
5.664AlaVal: 5.664 ± 0.594
1.69AlaTrp: 1.69 ± 0.263
1.782AlaTyr: 1.782 ± 0.226
0.0AlaXaa: 0.0 ± 0.0
Cys
0.777CysAla: 0.777 ± 0.23
0.137CysCys: 0.137 ± 0.077
0.594CysAsp: 0.594 ± 0.184
0.822CysGlu: 0.822 ± 0.212
0.274CysPhe: 0.274 ± 0.107
0.868CysGly: 0.868 ± 0.256
0.183CysHis: 0.183 ± 0.099
0.274CysIle: 0.274 ± 0.107
0.457CysLys: 0.457 ± 0.157
0.64CysLeu: 0.64 ± 0.171
0.228CysMet: 0.228 ± 0.107
0.228CysAsn: 0.228 ± 0.119
0.64CysPro: 0.64 ± 0.199
0.32CysGln: 0.32 ± 0.126
0.594CysArg: 0.594 ± 0.183
0.914CysSer: 0.914 ± 0.205
0.274CysThr: 0.274 ± 0.101
0.411CysVal: 0.411 ± 0.132
0.046CysTrp: 0.046 ± 0.044
0.32CysTyr: 0.32 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
4.385AspAla: 4.385 ± 0.424
0.64AspCys: 0.64 ± 0.172
4.934AspAsp: 4.934 ± 0.869
4.614AspGlu: 4.614 ± 0.593
3.38AspPhe: 3.38 ± 0.337
4.431AspGly: 4.431 ± 0.442
1.416AspHis: 1.416 ± 0.269
3.38AspIle: 3.38 ± 0.424
2.878AspLys: 2.878 ± 0.457
4.842AspLeu: 4.842 ± 0.466
1.645AspMet: 1.645 ± 0.281
2.147AspAsn: 2.147 ± 0.288
4.066AspPro: 4.066 ± 0.465
2.193AspGln: 2.193 ± 0.324
3.563AspArg: 3.563 ± 0.452
5.025AspSer: 5.025 ± 0.517
3.198AspThr: 3.198 ± 0.483
3.837AspVal: 3.837 ± 0.484
1.233AspTrp: 1.233 ± 0.201
2.284AspTyr: 2.284 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
5.573GluAla: 5.573 ± 0.519
0.685GluCys: 0.685 ± 0.22
4.34GluAsp: 4.34 ± 0.565
4.248GluGlu: 4.248 ± 0.493
2.604GluPhe: 2.604 ± 0.377
4.111GluGly: 4.111 ± 0.509
1.416GluHis: 1.416 ± 0.298
4.842GluIle: 4.842 ± 0.75
3.243GluLys: 3.243 ± 0.534
5.984GluLeu: 5.984 ± 0.634
1.782GluMet: 1.782 ± 0.276
2.421GluAsn: 2.421 ± 0.373
2.512GluPro: 2.512 ± 0.338
2.284GluGln: 2.284 ± 0.321
4.157GluArg: 4.157 ± 0.449
4.066GluSer: 4.066 ± 0.446
4.066GluThr: 4.066 ± 0.427
4.157GluVal: 4.157 ± 0.513
1.005GluTrp: 1.005 ± 0.262
2.147GluTyr: 2.147 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.558PheAla: 2.558 ± 0.345
0.137PheCys: 0.137 ± 0.075
2.512PheAsp: 2.512 ± 0.314
3.015PheGlu: 3.015 ± 0.389
1.279PhePhe: 1.279 ± 0.264
4.659PheGly: 4.659 ± 0.535
0.731PheHis: 0.731 ± 0.198
1.69PheIle: 1.69 ± 0.208
2.056PheLys: 2.056 ± 0.359
2.467PheLeu: 2.467 ± 0.338
0.548PheMet: 0.548 ± 0.149
1.919PheAsn: 1.919 ± 0.358
1.69PhePro: 1.69 ± 0.208
1.645PheGln: 1.645 ± 0.238
1.873PheArg: 1.873 ± 0.33
2.558PheSer: 2.558 ± 0.347
3.015PheThr: 3.015 ± 0.367
2.741PheVal: 2.741 ± 0.406
0.685PheTrp: 0.685 ± 0.148
1.188PheTyr: 1.188 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
5.208GlyAla: 5.208 ± 0.781
0.594GlyCys: 0.594 ± 0.164
4.385GlyAsp: 4.385 ± 0.564
3.974GlyGlu: 3.974 ± 0.375
3.472GlyPhe: 3.472 ± 0.417
6.761GlyGly: 6.761 ± 0.765
1.507GlyHis: 1.507 ± 0.273
4.888GlyIle: 4.888 ± 0.749
4.477GlyLys: 4.477 ± 0.534
5.299GlyLeu: 5.299 ± 0.468
1.919GlyMet: 1.919 ± 0.339
4.066GlyAsn: 4.066 ± 0.384
3.198GlyPro: 3.198 ± 0.326
4.066GlyGln: 4.066 ± 0.544
4.111GlyArg: 4.111 ± 0.407
4.842GlySer: 4.842 ± 0.683
5.208GlyThr: 5.208 ± 0.63
4.796GlyVal: 4.796 ± 0.44
1.599GlyTrp: 1.599 ± 0.183
3.289GlyTyr: 3.289 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.233HisAla: 1.233 ± 0.303
0.183HisCys: 0.183 ± 0.093
1.051HisAsp: 1.051 ± 0.244
1.37HisGlu: 1.37 ± 0.246
0.731HisPhe: 0.731 ± 0.19
1.279HisGly: 1.279 ± 0.241
0.274HisHis: 0.274 ± 0.107
1.188HisIle: 1.188 ± 0.244
1.051HisLys: 1.051 ± 0.216
1.37HisLeu: 1.37 ± 0.272
0.594HisMet: 0.594 ± 0.164
0.502HisAsn: 0.502 ± 0.168
1.188HisPro: 1.188 ± 0.261
0.959HisGln: 0.959 ± 0.222
1.005HisArg: 1.005 ± 0.232
1.507HisSer: 1.507 ± 0.221
0.777HisThr: 0.777 ± 0.194
1.233HisVal: 1.233 ± 0.215
0.32HisTrp: 0.32 ± 0.126
1.005HisTyr: 1.005 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.934IleAla: 4.934 ± 0.457
0.502IleCys: 0.502 ± 0.156
4.248IleAsp: 4.248 ± 0.397
4.111IleGlu: 4.111 ± 0.552
2.238IlePhe: 2.238 ± 0.306
3.7IleGly: 3.7 ± 0.437
1.142IleHis: 1.142 ± 0.26
3.654IleIle: 3.654 ± 0.403
2.878IleLys: 2.878 ± 0.346
4.248IleLeu: 4.248 ± 0.385
1.188IleMet: 1.188 ± 0.236
2.467IleAsn: 2.467 ± 0.363
3.517IlePro: 3.517 ± 0.626
2.649IleGln: 2.649 ± 0.409
3.654IleArg: 3.654 ± 0.317
3.929IleSer: 3.929 ± 0.424
3.061IleThr: 3.061 ± 0.336
3.792IleVal: 3.792 ± 0.426
1.051IleTrp: 1.051 ± 0.249
1.416IleTyr: 1.416 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
6.076LysAla: 6.076 ± 0.468
0.822LysCys: 0.822 ± 0.227
3.289LysAsp: 3.289 ± 0.39
3.517LysGlu: 3.517 ± 0.406
1.507LysPhe: 1.507 ± 0.247
4.157LysGly: 4.157 ± 0.532
0.868LysHis: 0.868 ± 0.186
3.106LysIle: 3.106 ± 0.299
3.7LysLys: 3.7 ± 0.509
4.157LysLeu: 4.157 ± 0.47
1.69LysMet: 1.69 ± 0.311
2.695LysAsn: 2.695 ± 0.324
3.243LysPro: 3.243 ± 0.456
2.238LysGln: 2.238 ± 0.288
3.792LysArg: 3.792 ± 0.583
2.924LysSer: 2.924 ± 0.44
3.243LysThr: 3.243 ± 0.438
3.517LysVal: 3.517 ± 0.363
0.914LysTrp: 0.914 ± 0.198
1.599LysTyr: 1.599 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
6.761LeuAla: 6.761 ± 0.48
0.685LeuCys: 0.685 ± 0.18
4.614LeuAsp: 4.614 ± 0.443
5.573LeuGlu: 5.573 ± 0.554
2.787LeuPhe: 2.787 ± 0.379
6.487LeuGly: 6.487 ± 0.697
1.279LeuHis: 1.279 ± 0.226
3.837LeuIle: 3.837 ± 0.326
4.934LeuLys: 4.934 ± 0.383
5.801LeuLeu: 5.801 ± 0.592
1.827LeuMet: 1.827 ± 0.423
3.974LeuAsn: 3.974 ± 0.299
4.34LeuPro: 4.34 ± 0.469
3.106LeuGln: 3.106 ± 0.275
4.431LeuArg: 4.431 ± 0.396
5.482LeuSer: 5.482 ± 0.41
3.929LeuThr: 3.929 ± 0.289
4.751LeuVal: 4.751 ± 0.459
1.416LeuTrp: 1.416 ± 0.248
1.096LeuTyr: 1.096 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
2.421MetAla: 2.421 ± 0.442
0.274MetCys: 0.274 ± 0.128
1.462MetAsp: 1.462 ± 0.259
0.685MetGlu: 0.685 ± 0.169
0.731MetPhe: 0.731 ± 0.151
1.325MetGly: 1.325 ± 0.224
0.457MetHis: 0.457 ± 0.129
1.279MetIle: 1.279 ± 0.272
1.507MetLys: 1.507 ± 0.247
1.553MetLeu: 1.553 ± 0.213
0.502MetMet: 0.502 ± 0.143
1.827MetAsn: 1.827 ± 0.325
1.416MetPro: 1.416 ± 0.255
1.462MetGln: 1.462 ± 0.308
1.553MetArg: 1.553 ± 0.295
2.421MetSer: 2.421 ± 0.356
2.375MetThr: 2.375 ± 0.339
1.416MetVal: 1.416 ± 0.218
0.183MetTrp: 0.183 ± 0.094
0.457MetTyr: 0.457 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
3.563AsnAla: 3.563 ± 0.462
0.411AsnCys: 0.411 ± 0.127
2.741AsnAsp: 2.741 ± 0.389
3.335AsnGlu: 3.335 ± 0.398
1.37AsnPhe: 1.37 ± 0.285
3.746AsnGly: 3.746 ± 0.37
1.142AsnHis: 1.142 ± 0.308
1.827AsnIle: 1.827 ± 0.32
1.964AsnLys: 1.964 ± 0.315
3.563AsnLeu: 3.563 ± 0.417
0.868AsnMet: 0.868 ± 0.21
1.69AsnAsn: 1.69 ± 0.339
3.426AsnPro: 3.426 ± 0.404
1.919AsnGln: 1.919 ± 0.317
3.152AsnArg: 3.152 ± 0.389
3.106AsnSer: 3.106 ± 0.402
2.01AsnThr: 2.01 ± 0.281
2.832AsnVal: 2.832 ± 0.296
1.051AsnTrp: 1.051 ± 0.225
1.645AsnTyr: 1.645 ± 0.309
0.0AsnXaa: 0.0 ± 0.0
Pro
2.649ProAla: 2.649 ± 0.311
0.365ProCys: 0.365 ± 0.155
3.106ProAsp: 3.106 ± 0.317
4.157ProGlu: 4.157 ± 0.551
2.193ProPhe: 2.193 ± 0.285
4.294ProGly: 4.294 ± 0.378
1.005ProHis: 1.005 ± 0.192
2.924ProIle: 2.924 ± 0.428
3.426ProLys: 3.426 ± 0.372
3.792ProLeu: 3.792 ± 0.372
1.599ProMet: 1.599 ± 0.254
2.33ProAsn: 2.33 ± 0.332
2.101ProPro: 2.101 ± 0.283
1.416ProGln: 1.416 ± 0.323
2.056ProArg: 2.056 ± 0.328
3.335ProSer: 3.335 ± 0.312
2.421ProThr: 2.421 ± 0.345
3.198ProVal: 3.198 ± 0.412
1.279ProTrp: 1.279 ± 0.295
1.873ProTyr: 1.873 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
3.563GlnAla: 3.563 ± 0.666
0.274GlnCys: 0.274 ± 0.112
2.056GlnAsp: 2.056 ± 0.234
2.375GlnGlu: 2.375 ± 0.301
1.462GlnPhe: 1.462 ± 0.325
3.746GlnGly: 3.746 ± 0.608
0.594GlnHis: 0.594 ± 0.169
2.787GlnIle: 2.787 ± 0.38
1.827GlnLys: 1.827 ± 0.24
3.152GlnLeu: 3.152 ± 0.365
1.553GlnMet: 1.553 ± 0.259
2.147GlnAsn: 2.147 ± 0.419
1.416GlnPro: 1.416 ± 0.233
2.101GlnGln: 2.101 ± 0.586
2.193GlnArg: 2.193 ± 0.353
2.558GlnSer: 2.558 ± 0.333
1.873GlnThr: 1.873 ± 0.292
3.335GlnVal: 3.335 ± 0.341
0.777GlnTrp: 0.777 ± 0.2
0.959GlnTyr: 0.959 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
4.02ArgAla: 4.02 ± 0.441
0.914ArgCys: 0.914 ± 0.21
3.654ArgAsp: 3.654 ± 0.494
3.792ArgGlu: 3.792 ± 0.512
2.558ArgPhe: 2.558 ± 0.377
3.198ArgGly: 3.198 ± 0.373
1.188ArgHis: 1.188 ± 0.26
3.7ArgIle: 3.7 ± 0.4
3.974ArgLys: 3.974 ± 0.437
5.208ArgLeu: 5.208 ± 0.53
1.462ArgMet: 1.462 ± 0.351
2.832ArgAsn: 2.832 ± 0.38
2.878ArgPro: 2.878 ± 0.416
2.467ArgGln: 2.467 ± 0.351
3.335ArgArg: 3.335 ± 0.546
2.604ArgSer: 2.604 ± 0.382
2.558ArgThr: 2.558 ± 0.356
4.431ArgVal: 4.431 ± 0.433
1.096ArgTrp: 1.096 ± 0.251
1.736ArgTyr: 1.736 ± 0.38
0.0ArgXaa: 0.0 ± 0.0
Ser
5.664SerAla: 5.664 ± 0.431
0.457SerCys: 0.457 ± 0.161
3.974SerAsp: 3.974 ± 0.45
4.02SerGlu: 4.02 ± 0.464
2.558SerPhe: 2.558 ± 0.328
7.263SerGly: 7.263 ± 0.979
1.005SerHis: 1.005 ± 0.246
3.792SerIle: 3.792 ± 0.423
3.974SerLys: 3.974 ± 0.365
5.436SerLeu: 5.436 ± 0.529
2.101SerMet: 2.101 ± 0.29
2.787SerAsn: 2.787 ± 0.388
2.512SerPro: 2.512 ± 0.391
2.787SerGln: 2.787 ± 0.29
2.969SerArg: 2.969 ± 0.363
5.253SerSer: 5.253 ± 0.733
3.609SerThr: 3.609 ± 0.455
3.792SerVal: 3.792 ± 0.401
1.599SerTrp: 1.599 ± 0.277
1.782SerTyr: 1.782 ± 0.249
0.0SerXaa: 0.0 ± 0.0
Thr
4.02ThrAla: 4.02 ± 0.512
0.502ThrCys: 0.502 ± 0.136
3.106ThrAsp: 3.106 ± 0.368
2.969ThrGlu: 2.969 ± 0.342
2.741ThrPhe: 2.741 ± 0.346
4.34ThrGly: 4.34 ± 0.439
0.868ThrHis: 0.868 ± 0.18
3.883ThrIle: 3.883 ± 0.52
3.746ThrLys: 3.746 ± 0.338
4.385ThrLeu: 4.385 ± 0.492
1.325ThrMet: 1.325 ± 0.219
2.193ThrAsn: 2.193 ± 0.325
3.243ThrPro: 3.243 ± 0.357
2.056ThrGln: 2.056 ± 0.338
2.787ThrArg: 2.787 ± 0.384
3.609ThrSer: 3.609 ± 0.458
2.649ThrThr: 2.649 ± 0.465
3.106ThrVal: 3.106 ± 0.402
1.325ThrTrp: 1.325 ± 0.205
1.873ThrTyr: 1.873 ± 0.192
0.0ThrXaa: 0.0 ± 0.0
Val
5.573ValAla: 5.573 ± 0.53
0.502ValCys: 0.502 ± 0.177
5.116ValAsp: 5.116 ± 0.588
5.253ValGlu: 5.253 ± 0.583
2.832ValPhe: 2.832 ± 0.322
4.385ValGly: 4.385 ± 0.443
0.731ValHis: 0.731 ± 0.165
3.654ValIle: 3.654 ± 0.349
2.695ValLys: 2.695 ± 0.373
4.294ValLeu: 4.294 ± 0.549
1.37ValMet: 1.37 ± 0.264
3.289ValAsn: 3.289 ± 0.437
2.924ValPro: 2.924 ± 0.38
1.69ValGln: 1.69 ± 0.309
4.111ValArg: 4.111 ± 0.468
5.482ValSer: 5.482 ± 0.48
3.837ValThr: 3.837 ± 0.44
4.705ValVal: 4.705 ± 0.593
0.822ValTrp: 0.822 ± 0.191
1.645ValTyr: 1.645 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
1.37TrpAla: 1.37 ± 0.29
0.091TrpCys: 0.091 ± 0.061
1.416TrpAsp: 1.416 ± 0.264
1.233TrpGlu: 1.233 ± 0.269
0.32TrpPhe: 0.32 ± 0.106
1.188TrpGly: 1.188 ± 0.259
0.685TrpHis: 0.685 ± 0.187
1.279TrpIle: 1.279 ± 0.257
1.188TrpLys: 1.188 ± 0.286
1.507TrpLeu: 1.507 ± 0.283
0.274TrpMet: 0.274 ± 0.127
1.051TrpAsn: 1.051 ± 0.172
0.731TrpPro: 0.731 ± 0.226
0.822TrpGln: 0.822 ± 0.194
1.188TrpArg: 1.188 ± 0.248
1.37TrpSer: 1.37 ± 0.244
1.005TrpThr: 1.005 ± 0.247
1.37TrpVal: 1.37 ± 0.266
0.64TrpTrp: 0.64 ± 0.191
0.64TrpTyr: 0.64 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.375TyrAla: 2.375 ± 0.381
0.365TyrCys: 0.365 ± 0.148
2.284TyrAsp: 2.284 ± 0.346
1.645TyrGlu: 1.645 ± 0.299
1.005TyrPhe: 1.005 ± 0.225
2.924TyrGly: 2.924 ± 0.362
0.64TyrHis: 0.64 ± 0.158
1.325TyrIle: 1.325 ± 0.253
1.416TyrLys: 1.416 ± 0.325
2.832TyrLeu: 2.832 ± 0.325
0.32TyrMet: 0.32 ± 0.108
1.096TyrAsn: 1.096 ± 0.255
1.919TyrPro: 1.919 ± 0.346
1.142TyrGln: 1.142 ± 0.223
2.649TyrArg: 2.649 ± 0.427
1.279TyrSer: 1.279 ± 0.238
1.051TyrThr: 1.051 ± 0.206
1.827TyrVal: 1.827 ± 0.309
0.64TyrTrp: 0.64 ± 0.195
0.822TyrTyr: 0.822 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 121 proteins (21892 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski