Subdoligranulum sp. 4_3_54A2FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Subdoligranulum; unclassified Subdoligranulum

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4088 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9RZ40|G9RZ40_9FIRM Ribosomal RNA large subunit methyltransferase H OS=Subdoligranulum sp. 4_3_54A2FAA OX=665956 GN=rlmH PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.32KK3 pKa = 10.15FLSAALALCLAFSLAACGGSGSSTPASTAPSGGTAGSGAAIKK45 pKa = 10.31IGGIGPVTGSTAVYY59 pKa = 10.39GNAVKK64 pKa = 10.71NGAEE68 pKa = 3.78IAIAEE73 pKa = 4.17INALGGQQYY82 pKa = 9.75EE83 pKa = 4.5MKK85 pKa = 10.79FEE87 pKa = 5.07DD88 pKa = 5.22DD89 pKa = 3.68EE90 pKa = 5.95NDD92 pKa = 3.22AEE94 pKa = 4.58KK95 pKa = 10.65AINAYY100 pKa = 10.54NSLKK104 pKa = 10.63DD105 pKa = 3.09WGMQILMGTVTSNPCIAVAAEE126 pKa = 3.95TANDD130 pKa = 3.44NMFQLTPSGSAEE142 pKa = 3.79GCIANDD148 pKa = 3.07NAFRR152 pKa = 11.84VCFSDD157 pKa = 4.11PEE159 pKa = 4.17QGTLSADD166 pKa = 3.68YY167 pKa = 10.51IVEE170 pKa = 3.95NSLAAKK176 pKa = 9.92VAVIYY181 pKa = 10.42DD182 pKa = 3.41SSDD185 pKa = 3.39PYY187 pKa = 11.48SSGIHH192 pKa = 6.31DD193 pKa = 4.22AFVTEE198 pKa = 4.17AQEE201 pKa = 4.06KK202 pKa = 8.61GLEE205 pKa = 4.3VVSDD209 pKa = 3.69EE210 pKa = 5.56AFTADD215 pKa = 3.31SNKK218 pKa = 10.56DD219 pKa = 3.2FTVQIQKK226 pKa = 10.2AQSAGAEE233 pKa = 4.23LVFLPIYY240 pKa = 8.57YY241 pKa = 9.49TEE243 pKa = 4.69ASLILTQADD252 pKa = 3.8KK253 pKa = 10.88IGYY256 pKa = 8.18EE257 pKa = 4.14PVFFGCDD264 pKa = 3.37GLDD267 pKa = 3.21GLVSVEE273 pKa = 4.68NFDD276 pKa = 3.43TSLAEE281 pKa = 4.87DD282 pKa = 4.02VMLMTPFTASATDD295 pKa = 3.52DD296 pKa = 3.29KK297 pKa = 11.23TAAFVKK303 pKa = 10.22AYY305 pKa = 10.49NEE307 pKa = 4.44AYY309 pKa = 10.41GEE311 pKa = 4.43DD312 pKa = 4.09PNQFAADD319 pKa = 3.99AYY321 pKa = 9.65DD322 pKa = 4.66AIYY325 pKa = 9.98AIHH328 pKa = 6.69AAVEE332 pKa = 4.35KK333 pKa = 10.95AGVTADD339 pKa = 3.45MDD341 pKa = 3.76ASAICDD347 pKa = 3.59ALKK350 pKa = 10.69AAFTEE355 pKa = 4.32ITLEE359 pKa = 4.01GLTGKK364 pKa = 10.82GITWDD369 pKa = 3.44ASGAPAKK376 pKa = 10.72SPLVYY381 pKa = 9.97IIKK384 pKa = 10.5DD385 pKa = 3.64GVYY388 pKa = 8.96TAAA391 pKa = 4.95

Molecular weight: 40.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9RT72|G9RT72_9FIRM CotJB domain-containing protein OS=Subdoligranulum sp. 4_3_54A2FAA OX=665956 GN=HMPREF1032_02756 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.8KK9 pKa = 7.95RR10 pKa = 11.84HH11 pKa = 5.88RR12 pKa = 11.84ATKK15 pKa = 9.91HH16 pKa = 4.97GFLARR21 pKa = 11.84SKK23 pKa = 10.53SSNGRR28 pKa = 11.84KK29 pKa = 9.13VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84AKK41 pKa = 10.44LAAA44 pKa = 4.52

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4088

0

4088

1219814

24

3194

298.4

32.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.568 ± 0.058

1.734 ± 0.017

5.398 ± 0.028

6.637 ± 0.04

3.998 ± 0.027

7.766 ± 0.039

1.82 ± 0.019

5.216 ± 0.043

4.797 ± 0.033

9.629 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.021

3.418 ± 0.025

4.249 ± 0.029

3.39 ± 0.027

5.839 ± 0.041

5.355 ± 0.029

5.408 ± 0.034

7.108 ± 0.032

1.151 ± 0.015

3.727 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski