Eel virus European X
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3P8Y9|E3P8Y9_9RHAB Matrix protein OS=Eel virus European X OX=685443 GN=M PE=4 SV=1
MM1 pKa = 7.7 LSSNKK6 pKa = 9.74 NKK8 pKa = 10.54 NKK10 pKa = 9.91 FDD12 pKa = 4.06 LEE14 pKa = 4.66 GIQLLAKK21 pKa = 9.49 GVKK24 pKa = 9.47 NAGEE28 pKa = 4.39 SIDD31 pKa = 3.71 RR32 pKa = 11.84 ATDD35 pKa = 3.29 EE36 pKa = 4.54 KK37 pKa = 11.1 PEE39 pKa = 4.25 HH40 pKa = 6.59 LSDD43 pKa = 3.96 AFSKK47 pKa = 8.74 YY48 pKa = 8.33 TEE50 pKa = 4.15 YY51 pKa = 11.13 LSNEE55 pKa = 4.18 SKK57 pKa = 11.03 EE58 pKa = 4.13 EE59 pKa = 3.91 EE60 pKa = 3.87 EE61 pKa = 5.03 DD62 pKa = 3.84 FQFEE66 pKa = 4.08 QVDD69 pKa = 3.61 YY70 pKa = 10.89 GFQEE74 pKa = 4.35 SPEE77 pKa = 4.46 SNNPLQEE84 pKa = 3.7 SWIAKK89 pKa = 9.99 DD90 pKa = 4.5 DD91 pKa = 4.23 LEE93 pKa = 6.42 PDD95 pKa = 3.32 MGKK98 pKa = 9.4 SWVEE102 pKa = 3.85 YY103 pKa = 7.15 QAKK106 pKa = 9.7 MSFDD110 pKa = 3.62 YY111 pKa = 11.28 NEE113 pKa = 4.06 QVKK116 pKa = 8.14 PTVMRR121 pKa = 11.84 EE122 pKa = 3.8 INGLLGMLGGFAKK135 pKa = 10.61 FQDD138 pKa = 3.73 GKK140 pKa = 11.04 KK141 pKa = 9.96 EE142 pKa = 3.84 YY143 pKa = 10.82 LFYY146 pKa = 11.05 LPEE149 pKa = 5.29 KK150 pKa = 10.37 KK151 pKa = 10.17 EE152 pKa = 4.13 SEE154 pKa = 4.13 EE155 pKa = 3.85 RR156 pKa = 11.84 RR157 pKa = 11.84 SDD159 pKa = 3.99 KK160 pKa = 10.63 KK161 pKa = 10.39 QCPFKK166 pKa = 11.23 VDD168 pKa = 3.41 VTPKK172 pKa = 9.75 QDD174 pKa = 3.58 PKK176 pKa = 10.15 ITPTPEE182 pKa = 3.98 KK183 pKa = 10.08 EE184 pKa = 4.35 PKK186 pKa = 9.95 HH187 pKa = 6.35 LPTSAAGKK195 pKa = 10.21 KK196 pKa = 8.11 ITEE199 pKa = 4.32 EE200 pKa = 3.81 AAVMQGFWMDD210 pKa = 3.21 GMRR213 pKa = 11.84 LTEE216 pKa = 4.1 KK217 pKa = 10.39 TSGKK221 pKa = 9.88 YY222 pKa = 10.29 CLFFPQKK229 pKa = 9.69 MGWSQAEE236 pKa = 4.38 WISKK240 pKa = 10.46 SEE242 pKa = 4.59 DD243 pKa = 3.04 INPRR247 pKa = 11.84 TLAHH251 pKa = 7.51 DD252 pKa = 3.38 IFKK255 pKa = 10.62 WMVSKK260 pKa = 10.77 SPKK263 pKa = 8.38 RR264 pKa = 11.84 ATYY267 pKa = 8.97 LRR269 pKa = 11.84 KK270 pKa = 10.42 YY271 pKa = 9.04 MVEE274 pKa = 4.0 EE275 pKa = 4.06
Molecular weight: 31.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.248
IPC2_protein 5.321
IPC_protein 5.232
Toseland 5.169
ProMoST 5.258
Dawson 5.194
Bjellqvist 5.347
Wikipedia 5.041
Rodwell 5.13
Grimsley 5.08
Solomon 5.194
Lehninger 5.156
Nozaki 5.308
DTASelect 5.423
Thurlkill 5.156
EMBOSS 5.08
Sillero 5.397
Patrickios 4.418
IPC_peptide 5.207
IPC2_peptide 5.385
IPC2.peptide.svr19 5.475
Protein with the highest isoelectric point:
>tr|E3P8Z2|E3P8Z2_9RHAB Putative coat protein OS=Eel virus European X OX=685443 GN=C PE=4 SV=1
MM1 pKa = 7.11 EE2 pKa = 5.53 RR3 pKa = 11.84 KK4 pKa = 9.94 NIFSIFQRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 6.97 SQKK17 pKa = 9.56 RR18 pKa = 11.84 EE19 pKa = 3.43 GLIRR23 pKa = 11.84 NNVLSRR29 pKa = 11.84 LMSPQNKK36 pKa = 6.21 TQKK39 pKa = 10.49 SLPHH43 pKa = 6.03 LRR45 pKa = 11.84 KK46 pKa = 9.68 SQNICPHH53 pKa = 5.34 QLPAKK58 pKa = 9.79 KK59 pKa = 10.11 SPKK62 pKa = 9.31 KK63 pKa = 10.51 QPP65 pKa = 3.17
Molecular weight: 7.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.178
IPC2_protein 10.306
IPC_protein 11.725
Toseland 11.945
ProMoST 12.384
Dawson 11.959
Bjellqvist 11.901
Wikipedia 12.384
Rodwell 12.018
Grimsley 11.989
Solomon 12.413
Lehninger 12.31
Nozaki 11.945
DTASelect 11.901
Thurlkill 11.945
EMBOSS 12.427
Sillero 11.945
Patrickios 11.74
IPC_peptide 12.413
IPC2_peptide 11.374
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3596
65
2075
599.3
68.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.617 ± 0.446
1.39 ± 0.197
5.395 ± 0.329
6.618 ± 0.566
3.921 ± 0.428
5.729 ± 0.386
2.614 ± 0.311
6.062 ± 0.472
6.618 ± 0.704
9.789 ± 0.614
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.781 ± 0.378
4.533 ± 0.259
4.505 ± 0.549
4.283 ± 0.262
5.089 ± 0.469
8.788 ± 0.463
5.562 ± 0.483
5.478 ± 0.597
1.974 ± 0.17
3.254 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here