Pudu puda papillomavirus 1
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9KI84|A0A1I9KI84_9PAPI Protein E6 OS=Pudu puda papillomavirus 1 OX=1747360 GN=E6 PE=3 SV=1
MM1 pKa = 6.7 VLKK4 pKa = 10.33 RR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.17 RR8 pKa = 11.84 AAAEE12 pKa = 3.62 DD13 pKa = 4.94 LYY15 pKa = 11.0 RR16 pKa = 11.84 QCQLGGDD23 pKa = 4.38 CPPDD27 pKa = 3.05 IKK29 pKa = 11.25 NKK31 pKa = 9.8 IEE33 pKa = 4.37 HH34 pKa = 5.77 NTIADD39 pKa = 5.48 LILKK43 pKa = 8.86 WGSLGVFLGGLGIGTGRR60 pKa = 11.84 GTGGRR65 pKa = 11.84 FGYY68 pKa = 10.38 RR69 pKa = 11.84 PLPEE73 pKa = 4.18 PAAPGGRR80 pKa = 11.84 PGATLVRR87 pKa = 11.84 PTIPVDD93 pKa = 3.44 TVTPDD98 pKa = 3.16 ITPVDD103 pKa = 3.9 ALDD106 pKa = 4.03 ASSPSIVPLTDD117 pKa = 3.46 AGTDD121 pKa = 3.45 SSIVEE126 pKa = 4.71 VIAEE130 pKa = 4.08 AQPNGGPNAGSTEE143 pKa = 4.02 PPSIEE148 pKa = 4.23 VTPEE152 pKa = 3.78 PPPKK156 pKa = 10.04 KK157 pKa = 9.93 IRR159 pKa = 11.84 VTKK162 pKa = 9.77 SQHH165 pKa = 5.74 PNPVYY170 pKa = 10.58 DD171 pKa = 4.49 FSYY174 pKa = 11.11 DD175 pKa = 3.42 GTEE178 pKa = 4.09 TSHH181 pKa = 6.32 TGQSFVHH188 pKa = 6.18 MGSSAQYY195 pKa = 10.02 IGGEE199 pKa = 4.14 VTGEE203 pKa = 4.04 EE204 pKa = 4.57 IEE206 pKa = 4.2 LSTWSRR212 pKa = 11.84 PGEE215 pKa = 3.86 ATGDD219 pKa = 3.5 SFSFNTVEE227 pKa = 4.23 TGFGTSTPAQATVSAPRR244 pKa = 11.84 LYY246 pKa = 10.67 GRR248 pKa = 11.84 RR249 pKa = 11.84 IDD251 pKa = 3.61 QVRR254 pKa = 11.84 VSDD257 pKa = 3.72 TAFLTRR263 pKa = 11.84 PDD265 pKa = 3.6 TLIVFDD271 pKa = 4.8 NPTFNPEE278 pKa = 3.48 VTQVFEE284 pKa = 4.15 QDD286 pKa = 3.67 LEE288 pKa = 4.23 EE289 pKa = 4.55 LAAAPDD295 pKa = 4.31 PNFQDD300 pKa = 3.43 VNRR303 pKa = 11.84 LSRR306 pKa = 11.84 PYY308 pKa = 10.54 YY309 pKa = 9.1 SQTNEE314 pKa = 2.86 GRR316 pKa = 11.84 IRR318 pKa = 11.84 VSRR321 pKa = 11.84 VGSRR325 pKa = 11.84 GTLTTRR331 pKa = 11.84 SGIRR335 pKa = 11.84 IGPTVHH341 pKa = 7.02 FFQDD345 pKa = 3.94 LSSIAPGDD353 pKa = 3.75 TVIQEE358 pKa = 4.43 SLGEE362 pKa = 4.05 VSGSHH367 pKa = 6.84 TIYY370 pKa = 10.23 HH371 pKa = 5.89 GHH373 pKa = 5.87 SSEE376 pKa = 4.07 EE377 pKa = 3.97 FEE379 pKa = 4.98 MIDD382 pKa = 3.94 LAPEE386 pKa = 3.96 SEE388 pKa = 4.76 IYY390 pKa = 10.71 SEE392 pKa = 5.99 DD393 pKa = 3.72 DD394 pKa = 3.61 LLDD397 pKa = 4.69 HH398 pKa = 7.22 IEE400 pKa = 4.09 EE401 pKa = 4.45 VGDD404 pKa = 3.67 NLQLVIGGGRR414 pKa = 11.84 RR415 pKa = 11.84 QPPQSVPTFKK425 pKa = 10.38 TGLQGAGGIDD435 pKa = 3.8 VIYY438 pKa = 10.04 PNYY441 pKa = 9.82 PKK443 pKa = 10.23 IPGEE447 pKa = 4.11 FEE449 pKa = 3.96 PPAVPINPSGDD460 pKa = 3.42 TPEE463 pKa = 5.73 IIVTFLDD470 pKa = 3.16 SSYY473 pKa = 11.03 TVADD477 pKa = 3.69 PSLFRR482 pKa = 11.84 LRR484 pKa = 11.84 KK485 pKa = 7.31 RR486 pKa = 11.84 RR487 pKa = 11.84 KK488 pKa = 9.25 RR489 pKa = 11.84 RR490 pKa = 11.84 FHH492 pKa = 6.4
Molecular weight: 53.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.928
IPC2_protein 4.914
IPC_protein 4.863
Toseland 4.762
ProMoST 4.965
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.711
Rodwell 4.736
Grimsley 4.685
Solomon 4.838
Lehninger 4.787
Nozaki 4.952
DTASelect 5.118
Thurlkill 4.762
EMBOSS 4.736
Sillero 5.016
Patrickios 4.139
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 4.966
Protein with the highest isoelectric point:
>tr|A0A1I9KXY4|A0A1I9KXY4_9PAPI Replication protein E1 OS=Pudu puda papillomavirus 1 OX=1747360 GN=E1 PE=3 SV=1
MM1 pKa = 6.94 EE2 pKa = 4.74 TLRR5 pKa = 11.84 DD6 pKa = 3.54 RR7 pKa = 11.84 LDD9 pKa = 3.49 AVQEE13 pKa = 4.1 EE14 pKa = 5.17 LLTLYY19 pKa = 10.88 EE20 pKa = 4.63 EE21 pKa = 5.17 GNTDD25 pKa = 2.77 IDD27 pKa = 4.01 SQIKK31 pKa = 8.98 HH32 pKa = 5.36 WNLLRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.03 HH40 pKa = 6.33 VLLHH44 pKa = 6.08 FARR47 pKa = 11.84 KK48 pKa = 8.91 QGIYY52 pKa = 10.28 RR53 pKa = 11.84 LGLQTVPPLQVSQQKK68 pKa = 10.32 AKK70 pKa = 10.43 EE71 pKa = 4.19 AIEE74 pKa = 4.11 MVLTLQSLKK83 pKa = 10.28 KK84 pKa = 10.17 SPYY87 pKa = 10.46 GEE89 pKa = 4.33 EE90 pKa = 3.71 TWTLADD96 pKa = 3.37 TSRR99 pKa = 11.84 EE100 pKa = 4.03 LFEE103 pKa = 4.85 TPPKK107 pKa = 10.83 NCFKK111 pKa = 10.91 KK112 pKa = 9.83 HH113 pKa = 4.62 GASVEE118 pKa = 3.79 VRR120 pKa = 11.84 YY121 pKa = 10.57 DD122 pKa = 3.5 GSAANSMLYY131 pKa = 8.78 TAWSDD136 pKa = 2.61 IYY138 pKa = 11.29 YY139 pKa = 8.3 QTDD142 pKa = 3.79 YY143 pKa = 11.29 SWEE146 pKa = 4.09 KK147 pKa = 8.24 TQGHH151 pKa = 5.69 VDD153 pKa = 3.46 YY154 pKa = 9.65 TGCYY158 pKa = 9.56 YY159 pKa = 10.96 YY160 pKa = 10.83 EE161 pKa = 5.6 DD162 pKa = 4.08 DD163 pKa = 4.4 VKK165 pKa = 10.69 IYY167 pKa = 9.91 YY168 pKa = 10.6 LKK170 pKa = 11.07 FEE172 pKa = 5.58 DD173 pKa = 4.26 DD174 pKa = 3.41 AARR177 pKa = 11.84 YY178 pKa = 8.48 SATGEE183 pKa = 4.09 WEE185 pKa = 3.88 VHH187 pKa = 5.22 YY188 pKa = 10.85 KK189 pKa = 10.57 NDD191 pKa = 2.93 IFSPVASVSSTSSWTPSPVAATSTGGTAEE220 pKa = 4.52 DD221 pKa = 3.95 TQQYY225 pKa = 8.36 PVPLDD230 pKa = 3.41 NTPATVKK237 pKa = 10.05 RR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 11.84 TGSPSRR246 pKa = 11.84 TTKK249 pKa = 10.17 RR250 pKa = 11.84 GRR252 pKa = 11.84 PRR254 pKa = 11.84 RR255 pKa = 11.84 KK256 pKa = 9.06 PRR258 pKa = 11.84 SSTPRR263 pKa = 11.84 GRR265 pKa = 11.84 FSAQEE270 pKa = 3.69 EE271 pKa = 4.6 GRR273 pKa = 11.84 RR274 pKa = 11.84 RR275 pKa = 11.84 SQQEE279 pKa = 3.19 EE280 pKa = 4.36 GQRR283 pKa = 11.84 QRR285 pKa = 11.84 RR286 pKa = 11.84 TQQRR290 pKa = 11.84 QQGEE294 pKa = 4.3 RR295 pKa = 11.84 ARR297 pKa = 11.84 SPRR300 pKa = 11.84 LPEE303 pKa = 3.54 EE304 pKa = 4.16 SARR307 pKa = 11.84 QVAEE311 pKa = 3.96 GAGRR315 pKa = 11.84 RR316 pKa = 11.84 HH317 pKa = 5.65 RR318 pKa = 11.84 RR319 pKa = 11.84 SRR321 pKa = 11.84 LDD323 pKa = 3.18 QLLADD328 pKa = 4.48 AADD331 pKa = 3.9 PPIVLLRR338 pKa = 11.84 GGANQLKK345 pKa = 9.22 CLRR348 pKa = 11.84 YY349 pKa = 9.28 RR350 pKa = 11.84 LIRR353 pKa = 11.84 KK354 pKa = 9.24 YY355 pKa = 10.68 SALFSLISSTWSWVGLQGPGGRR377 pKa = 11.84 NRR379 pKa = 11.84 LMLAFNGYY387 pKa = 7.36 QQRR390 pKa = 11.84 TDD392 pKa = 4.67 FINSVPLPPGVSVVLGTLQGII413 pKa = 4.3
Molecular weight: 47.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.875
IPC2_protein 9.077
IPC_protein 9.151
Toseland 9.472
ProMoST 9.385
Dawson 9.809
Bjellqvist 9.575
Wikipedia 10.043
Rodwell 9.94
Grimsley 9.926
Solomon 9.838
Lehninger 9.78
Nozaki 9.531
DTASelect 9.56
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.736
Patrickios 4.838
IPC_peptide 9.823
IPC2_peptide 8.317
IPC2.peptide.svr19 7.95
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2249
86
609
374.8
42.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.514 ± 0.531
2.179 ± 1.0
5.78 ± 0.175
6.981 ± 0.459
4.357 ± 0.489
6.892 ± 1.035
1.823 ± 0.23
4.491 ± 0.507
4.936 ± 0.749
8.982 ± 1.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.601 ± 0.388
3.913 ± 0.563
5.958 ± 1.058
5.113 ± 0.401
6.447 ± 0.778
7.603 ± 0.508
6.803 ± 0.445
5.558 ± 0.459
1.734 ± 0.41
3.335 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here