Serinus canaria papillomavirus 1
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2P5B7|A0A2L2P5B7_9PAPI L2 OS=Serinus canaria papillomavirus 1 OX=2094713 PE=4 SV=1
MM1 pKa = 7.69 PRR3 pKa = 11.84 QPLWSPPVRR12 pKa = 11.84 QPEE15 pKa = 4.23 EE16 pKa = 3.84 NDD18 pKa = 2.85 EE19 pKa = 5.59 DD20 pKa = 4.05 IDD22 pKa = 4.25 FNLFIDD28 pKa = 4.33 DD29 pKa = 4.58 SEE31 pKa = 4.82 NNSSDD36 pKa = 4.76 SEE38 pKa = 4.53 GSWEE42 pKa = 4.55 LCLTVQGSEE51 pKa = 4.12 HH52 pKa = 6.66 VFDD55 pKa = 5.02 GLSEE59 pKa = 4.07 DD60 pKa = 4.02 VEE62 pKa = 4.74 GGEE65 pKa = 4.25 SDD67 pKa = 5.34 SYY69 pKa = 11.3 IQGLDD74 pKa = 3.53 VPDD77 pKa = 4.94 GVCPSLVCTEE87 pKa = 4.78 DD88 pKa = 3.55 PSFSSPEE95 pKa = 4.12 LGIHH99 pKa = 6.67 PALTPGLFTTAPEE112 pKa = 4.4 VVPPPYY118 pKa = 9.88 RR119 pKa = 11.84 PPPPYY124 pKa = 9.84 PGLPPPYY131 pKa = 9.32 RR132 pKa = 11.84 PQPPPCRR139 pKa = 11.84 PPPPPCRR146 pKa = 11.84 PPPPPYY152 pKa = 10.04 RR153 pKa = 11.84 PPPPPYY159 pKa = 9.9 RR160 pKa = 11.84 PPPPPYY166 pKa = 9.94 RR167 pKa = 11.84 PPACPTPAYY176 pKa = 9.45 PEE178 pKa = 4.7 LPACRR183 pKa = 11.84 YY184 pKa = 5.58 TAPGIGSAYY193 pKa = 8.91 GQNPGPYY200 pKa = 8.5 GQNPSYY206 pKa = 10.13 EE207 pKa = 4.12 PSYY210 pKa = 10.28 EE211 pKa = 3.85 PSYY214 pKa = 10.46 QPSYY218 pKa = 9.79 QPSYY222 pKa = 9.8 QPSYY226 pKa = 9.97 QQDD229 pKa = 3.62 PYY231 pKa = 10.76 YY232 pKa = 10.52 GQNPGHH238 pKa = 6.07 GQDD241 pKa = 3.82 PASGQLPNGPPASAPYY257 pKa = 9.74 YY258 pKa = 10.22 PPDD261 pKa = 3.7 TLQVGLDD268 pKa = 3.65 VEE270 pKa = 4.45 DD271 pKa = 4.57 QEE273 pKa = 5.79 DD274 pKa = 4.55 GPGYY278 pKa = 10.36 SISTNWACTICGNPLTLTDD297 pKa = 4.24 LLMYY301 pKa = 10.15 EE302 pKa = 4.87 PVSQAQQLGVCYY314 pKa = 10.24 LCYY317 pKa = 10.24 SGQSLEE323 pKa = 5.17 DD324 pKa = 3.6 LFASS328 pKa = 4.24
Molecular weight: 35.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.901
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.91
Patrickios 0.006
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A2L2P5B0|A0A2L2P5B0_9PAPI Major capsid protein L1 OS=Serinus canaria papillomavirus 1 OX=2094713 GN=L1 PE=3 SV=1
MM1 pKa = 7.58 PMWTVLGLSDD11 pKa = 4.37 EE12 pKa = 4.78 VPDD15 pKa = 3.82 EE16 pKa = 4.42 VPVPVHH22 pKa = 5.86 EE23 pKa = 4.43 STRR26 pKa = 11.84 EE27 pKa = 3.99 DD28 pKa = 3.54 EE29 pKa = 4.4 VDD31 pKa = 3.25 RR32 pKa = 11.84 SLVSTPPPPDD42 pKa = 3.26 LSRR45 pKa = 11.84 YY46 pKa = 7.99 SPRR49 pKa = 11.84 PSAPRR54 pKa = 11.84 KK55 pKa = 9.16 GKK57 pKa = 7.3 GTRR60 pKa = 11.84 VVRR63 pKa = 11.84 PTRR66 pKa = 11.84 PGPSGRR72 pKa = 11.84 SSTQATQPRR81 pKa = 11.84 QPATSRR87 pKa = 11.84 QSVRR91 pKa = 11.84 QSRR94 pKa = 11.84 EE95 pKa = 3.48 GLKK98 pKa = 10.49 RR99 pKa = 11.84 PLQPPTPDD107 pKa = 2.72 EE108 pKa = 4.36 VGTVHH113 pKa = 7.01 SSSHH117 pKa = 5.93 HH118 pKa = 5.54 GGTRR122 pKa = 11.84 VDD124 pKa = 5.05 RR125 pKa = 11.84 LIQDD129 pKa = 3.34 ARR131 pKa = 11.84 DD132 pKa = 3.6 PPGLCFEE139 pKa = 5.03 GKK141 pKa = 8.43 TNQLKK146 pKa = 9.62 TIRR149 pKa = 11.84 QRR151 pKa = 11.84 IFGGQLPFEE160 pKa = 4.31 RR161 pKa = 11.84 VSTTWHH167 pKa = 5.48 WVKK170 pKa = 10.97 KK171 pKa = 10.45 DD172 pKa = 3.44 GEE174 pKa = 4.43 SDD176 pKa = 3.64 SKK178 pKa = 11.16 ILVVFKK184 pKa = 10.66 DD185 pKa = 3.15 KK186 pKa = 10.78 KK187 pKa = 10.98 DD188 pKa = 3.23 RR189 pKa = 11.84 DD190 pKa = 3.81 FFQKK194 pKa = 10.38 SFYY197 pKa = 10.27 VGSSGVRR204 pKa = 11.84 VFHH207 pKa = 7.11 CSLAGLL213 pKa = 4.12
Molecular weight: 23.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.151
IPC_protein 9.253
Toseland 10.292
ProMoST 9.809
Dawson 10.379
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.774
Grimsley 10.409
Solomon 10.438
Lehninger 10.423
Nozaki 10.292
DTASelect 9.984
Thurlkill 10.277
EMBOSS 10.672
Sillero 10.306
Patrickios 10.511
IPC_peptide 10.452
IPC2_peptide 8.565
IPC2.peptide.svr19 8.276
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2925
83
678
365.6
40.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.154 ± 0.635
2.12 ± 0.556
6.017 ± 0.315
5.709 ± 0.324
3.487 ± 0.36
7.556 ± 0.934
2.154 ± 0.324
3.897 ± 0.458
3.658 ± 0.777
8.308 ± 0.897
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.436 ± 0.226
3.179 ± 0.536
9.299 ± 1.807
4.821 ± 0.414
7.214 ± 0.679
8.068 ± 0.545
5.231 ± 0.646
6.359 ± 0.887
1.607 ± 0.132
3.726 ± 0.58
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here