Methanimicrococcus blatticola

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanimicrococcus

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484F462|A0A484F462_9EURY Tritrans polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific) OS=Methanimicrococcus blatticola OX=91560 GN=uppS PE=3 SV=1
MM1 pKa = 7.32SASDD5 pKa = 3.64KK6 pKa = 11.09KK7 pKa = 11.29GGFIDD12 pKa = 4.21SKK14 pKa = 10.66YY15 pKa = 10.56LNIVTAVFVVFAVLLVCILMYY36 pKa = 10.7NPSLLGIQSASAHH49 pKa = 6.86PDD51 pKa = 3.36YY52 pKa = 10.81EE53 pKa = 4.56DD54 pKa = 4.92KK55 pKa = 11.01IFNQSMITSISIDD68 pKa = 3.26MSEE71 pKa = 4.47EE72 pKa = 3.11NWMALLEE79 pKa = 4.43NPLTEE84 pKa = 4.5EE85 pKa = 4.45FYY87 pKa = 10.4MANVTINGEE96 pKa = 4.08TFYY99 pKa = 11.42SVGIRR104 pKa = 11.84TKK106 pKa = 11.12GMTSLSQVASSDD118 pKa = 3.05SDD120 pKa = 3.44RR121 pKa = 11.84YY122 pKa = 10.49SFKK125 pKa = 11.04LKK127 pKa = 10.48ADD129 pKa = 3.74TYY131 pKa = 11.09IDD133 pKa = 3.6GQTFFGLEE141 pKa = 3.73EE142 pKa = 3.8FVINNMYY149 pKa = 10.45QDD151 pKa = 3.62PSYY154 pKa = 10.08MKK156 pKa = 10.21EE157 pKa = 3.73YY158 pKa = 10.27LAYY161 pKa = 10.94DD162 pKa = 3.4MMTYY166 pKa = 9.77MGVASPLFNFADD178 pKa = 2.96ITMNGEE184 pKa = 3.55PWGLYY189 pKa = 9.68LAIEE193 pKa = 4.25VMEE196 pKa = 4.8EE197 pKa = 3.74DD198 pKa = 3.28FAMRR202 pKa = 11.84VYY204 pKa = 10.98GSDD207 pKa = 3.18FGQLYY212 pKa = 10.09KK213 pKa = 10.68PEE215 pKa = 4.01TMGMGGGNVGGNRR228 pKa = 11.84PEE230 pKa = 4.21GGGNFGGGMGITRR243 pKa = 11.84PEE245 pKa = 4.57DD246 pKa = 3.37GDD248 pKa = 3.7FDD250 pKa = 5.42GDD252 pKa = 3.52FGEE255 pKa = 4.23ISNRR259 pKa = 11.84NGQMIPIQGNQAMPTASITLATSADD284 pKa = 3.61NQTITVNGTTFEE296 pKa = 4.21TQEE299 pKa = 4.53DD300 pKa = 4.8GTFTEE305 pKa = 4.52EE306 pKa = 4.15DD307 pKa = 3.27RR308 pKa = 11.84AAIAAAMAEE317 pKa = 4.24AGNTRR322 pKa = 11.84QNFGGMGTGGGADD335 pKa = 4.14LVYY338 pKa = 10.49TDD340 pKa = 5.81DD341 pKa = 5.01NISSYY346 pKa = 10.26SQIFEE351 pKa = 3.9NAVFKK356 pKa = 10.63NAKK359 pKa = 9.7NSDD362 pKa = 3.7FNRR365 pKa = 11.84VITALKK371 pKa = 9.12YY372 pKa = 10.43LNAGEE377 pKa = 4.2EE378 pKa = 4.4LEE380 pKa = 4.55TYY382 pKa = 10.95VDD384 pKa = 3.22VDD386 pKa = 3.05HH387 pKa = 6.89TLRR390 pKa = 11.84YY391 pKa = 8.76FAVNTVVVNLDD402 pKa = 3.87SYY404 pKa = 11.98VSGLKK409 pKa = 10.11HH410 pKa = 6.6NYY412 pKa = 9.25YY413 pKa = 10.5LYY415 pKa = 10.79EE416 pKa = 5.45KK417 pKa = 10.07DD418 pKa = 3.51GQITILPWDD427 pKa = 3.67YY428 pKa = 11.59NLAFGAFQSGTASSTVNFPIDD449 pKa = 3.7TPVSGVSMEE458 pKa = 4.13DD459 pKa = 3.05RR460 pKa = 11.84PLIGVLLAEE469 pKa = 4.77DD470 pKa = 4.75EE471 pKa = 4.49YY472 pKa = 11.2MDD474 pKa = 5.62LYY476 pKa = 11.06HH477 pKa = 7.59EE478 pKa = 4.27YY479 pKa = 10.28LQEE482 pKa = 4.48IVDD485 pKa = 4.11EE486 pKa = 4.6YY487 pKa = 11.3FNSGYY492 pKa = 10.21YY493 pKa = 10.1DD494 pKa = 3.54NKK496 pKa = 9.52ITEE499 pKa = 4.6LDD501 pKa = 3.76DD502 pKa = 5.78LIGEE506 pKa = 4.41HH507 pKa = 6.22VKK509 pKa = 10.77NDD511 pKa = 3.09QTAFFTYY518 pKa = 10.22DD519 pKa = 3.11EE520 pKa = 4.63YY521 pKa = 10.77VTAVEE526 pKa = 4.1EE527 pKa = 4.01MRR529 pKa = 11.84VFGEE533 pKa = 4.18LRR535 pKa = 11.84AEE537 pKa = 4.5SIQGQLDD544 pKa = 3.52GTVPSTSEE552 pKa = 4.0DD553 pKa = 3.18QTANPDD559 pKa = 3.65ALIDD563 pKa = 3.87ASALNMSALGGMGGGMGGGMPGGGGRR589 pKa = 11.84MQGGNWPTQVTTPQAADD606 pKa = 4.01DD607 pKa = 4.52LSVPPTGTGAAPAA620 pKa = 4.28

Molecular weight: 67.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484F3W2|A0A484F3W2_9EURY UPF0219 protein C7391_1026 OS=Methanimicrococcus blatticola OX=91560 GN=C7391_1026 PE=3 SV=1
MM1 pKa = 7.51SKK3 pKa = 10.36NLKK6 pKa = 8.69GQKK9 pKa = 9.41KK10 pKa = 9.15RR11 pKa = 11.84LAKK14 pKa = 10.55AHH16 pKa = 5.14VQNARR21 pKa = 11.84VPVWVIIKK29 pKa = 7.41TKK31 pKa = 10.61RR32 pKa = 11.84NVTSHH37 pKa = 6.76PKK39 pKa = 8.23RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.63WRR44 pKa = 11.84RR45 pKa = 11.84SSMRR49 pKa = 11.84LKK51 pKa = 10.83

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1537

0

1537

476405

25

1890

310.0

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.749 ± 0.07

1.186 ± 0.034

6.068 ± 0.053

7.481 ± 0.075

4.286 ± 0.05

7.217 ± 0.074

1.54 ± 0.025

7.896 ± 0.059

6.994 ± 0.057

8.412 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.035

4.587 ± 0.064

3.879 ± 0.04

2.547 ± 0.031

3.767 ± 0.053

6.432 ± 0.065

5.83 ± 0.069

7.074 ± 0.06

0.789 ± 0.024

3.522 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski