Erwinia sp. Leaf53

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia; unclassified Erwinia

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4MT00|A0A0Q4MT00_9GAMM Uncharacterized protein OS=Erwinia sp. Leaf53 OX=1736225 GN=ASF13_06150 PE=4 SV=1
MM1 pKa = 6.57QTKK4 pKa = 8.56QQIFDD9 pKa = 4.11QIAQLATACHH19 pKa = 5.42EE20 pKa = 4.38HH21 pKa = 6.74ACSIDD26 pKa = 3.43VGPEE30 pKa = 3.54RR31 pKa = 11.84TQMFSVYY38 pKa = 10.32GVLHH42 pKa = 5.39NLGRR46 pKa = 11.84NGYY49 pKa = 9.01AEE51 pKa = 3.88QVEE54 pKa = 4.85FAMNPLLSGGDD65 pKa = 4.73DD66 pKa = 4.05DD67 pKa = 6.81DD68 pKa = 6.47DD69 pKa = 3.9WDD71 pKa = 4.52EE72 pKa = 6.1DD73 pKa = 4.35DD74 pKa = 5.59DD75 pKa = 4.45

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4MV87|A0A0Q4MV87_9GAMM Sensor protein QseC OS=Erwinia sp. Leaf53 OX=1736225 GN=ASF13_14595 PE=4 SV=1
MM1 pKa = 6.53QHH3 pKa = 7.0KK4 pKa = 10.58LPGRR8 pKa = 11.84QAGRR12 pKa = 11.84QAGRR16 pKa = 11.84QAGRR20 pKa = 11.84QAGRR24 pKa = 11.84QEE26 pKa = 4.18AGRR29 pKa = 11.84QAGRR33 pKa = 11.84QAGRR37 pKa = 11.84QAGRR41 pKa = 11.84QAGRR45 pKa = 11.84QAGRR49 pKa = 11.84QAGRR53 pKa = 11.84QDD55 pKa = 3.24RR56 pKa = 11.84QDD58 pKa = 3.4RR59 pKa = 11.84QGKK62 pKa = 8.78ARR64 pKa = 11.84QIRR67 pKa = 11.84RR68 pKa = 3.48

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4431

0

4431

1423473

36

2997

321.3

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.788 ± 0.042

0.985 ± 0.013

5.127 ± 0.027

5.258 ± 0.035

3.703 ± 0.026

7.748 ± 0.035

2.172 ± 0.02

5.191 ± 0.028

3.548 ± 0.032

11.435 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.017

3.37 ± 0.022

4.638 ± 0.024

4.916 ± 0.035

6.111 ± 0.035

6.101 ± 0.031

5.2 ± 0.032

7.048 ± 0.029

1.535 ± 0.015

2.617 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski