Amino acid dipepetide frequency for Erwinia sp. Leaf53

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.3AlaAla: 13.3 ± 0.16	1.112AlaCys: 1.112 ± 0.031	5.739AlaAsp: 5.739 ± 0.075	6.501AlaGlu: 6.501 ± 0.097	3.713AlaPhe: 3.713 ± 0.063	9.399AlaGly: 9.399 ± 0.101	1.786AlaHis: 1.786 ± 0.036	5.793AlaIle: 5.793 ± 0.077	3.292AlaLys: 3.292 ± 0.065	13.539AlaLeu: 13.539 ± 0.132	2.868AlaMet: 2.868 ± 0.048	2.736AlaAsn: 2.736 ± 0.046	4.012AlaPro: 4.012 ± 0.068	4.894AlaGln: 4.894 ± 0.068	6.412AlaArg: 6.412 ± 0.075	6.397AlaSer: 6.397 ± 0.078	5.166AlaThr: 5.166 ± 0.066	7.499AlaVal: 7.499 ± 0.077	1.738AlaTrp: 1.738 ± 0.036	1.982AlaTyr: 1.982 ± 0.042	0.003AlaXaa: 0.003 ± 0.002
Cys	0.923CysAla: 0.923 ± 0.025	0.18CysCys: 0.18 ± 0.012	0.558CysAsp: 0.558 ± 0.021	0.501CysGlu: 0.501 ± 0.019	0.387CysPhe: 0.387 ± 0.018	1.043CysGly: 1.043 ± 0.03	0.277CysHis: 0.277 ± 0.015	0.458CysIle: 0.458 ± 0.019	0.242CysLys: 0.242 ± 0.015	1.001CysLeu: 1.001 ± 0.03	0.211CysMet: 0.211 ± 0.013	0.285CysAsn: 0.285 ± 0.014	0.435CysPro: 0.435 ± 0.015	0.443CysGln: 0.443 ± 0.017	0.698CysArg: 0.698 ± 0.023	0.637CysSer: 0.637 ± 0.02	0.412CysThr: 0.412 ± 0.019	0.664CysVal: 0.664 ± 0.018	0.188CysTrp: 0.188 ± 0.013	0.308CysTyr: 0.308 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.798AspAla: 5.798 ± 0.067	0.464AspCys: 0.464 ± 0.021	2.915AspAsp: 2.915 ± 0.049	3.206AspGlu: 3.206 ± 0.049	2.124AspPhe: 2.124 ± 0.042	4.131AspGly: 4.131 ± 0.062	0.96AspHis: 0.96 ± 0.025	2.953AspIle: 2.953 ± 0.049	2.024AspLys: 2.024 ± 0.038	4.771AspLeu: 4.771 ± 0.052	1.131AspMet: 1.131 ± 0.03	2.006AspAsn: 2.006 ± 0.037	2.372AspPro: 2.372 ± 0.039	1.639AspGln: 1.639 ± 0.038	3.423AspArg: 3.423 ± 0.051	2.757AspSer: 2.757 ± 0.049	2.325AspThr: 2.325 ± 0.04	3.633AspVal: 3.633 ± 0.055	0.821AspTrp: 0.821 ± 0.025	1.817AspTyr: 1.817 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	5.418GluAla: 5.418 ± 0.086	0.405GluCys: 0.405 ± 0.017	2.104GluAsp: 2.104 ± 0.043	2.899GluGlu: 2.899 ± 0.049	1.721GluPhe: 1.721 ± 0.036	3.383GluGly: 3.383 ± 0.046	1.227GluHis: 1.227 ± 0.029	2.998GluIle: 2.998 ± 0.047	2.663GluLys: 2.663 ± 0.045	5.761GluLeu: 5.761 ± 0.074	1.576GluMet: 1.576 ± 0.033	1.978GluAsn: 1.978 ± 0.039	2.076GluPro: 2.076 ± 0.04	3.614GluGln: 3.614 ± 0.055	3.674GluArg: 3.674 ± 0.066	2.774GluSer: 2.774 ± 0.042	2.631GluThr: 2.631 ± 0.046	3.687GluVal: 3.687 ± 0.062	0.743GluTrp: 0.743 ± 0.024	1.251GluTyr: 1.251 ± 0.031	0.001GluXaa: 0.001 ± 0.001
Phe	3.815PheAla: 3.815 ± 0.052	0.483PheCys: 0.483 ± 0.018	2.339PheAsp: 2.339 ± 0.043	1.594PheGlu: 1.594 ± 0.034	1.556PhePhe: 1.556 ± 0.046	3.162PheGly: 3.162 ± 0.06	0.776PheHis: 0.776 ± 0.023	2.307PheIle: 2.307 ± 0.042	1.133PheLys: 1.133 ± 0.031	3.128PheLeu: 3.128 ± 0.058	0.915PheMet: 0.915 ± 0.025	1.566PheAsn: 1.566 ± 0.032	1.488PhePro: 1.488 ± 0.035	1.124PheGln: 1.124 ± 0.027	1.892PheArg: 1.892 ± 0.037	3.349PheSer: 3.349 ± 0.06	2.375PheThr: 2.375 ± 0.05	2.312PheVal: 2.312 ± 0.041	0.607PheTrp: 0.607 ± 0.022	1.114PheTyr: 1.114 ± 0.03	0.001PheXaa: 0.001 ± 0.001
Gly	6.805GlyAla: 6.805 ± 0.069	0.923GlyCys: 0.923 ± 0.029	3.886GlyAsp: 3.886 ± 0.057	4.67GlyGlu: 4.67 ± 0.057	3.272GlyPhe: 3.272 ± 0.057	6.013GlyGly: 6.013 ± 0.078	1.697GlyHis: 1.697 ± 0.041	4.722GlyIle: 4.722 ± 0.065	3.52GlyLys: 3.52 ± 0.057	8.138GlyLeu: 8.138 ± 0.094	2.313GlyMet: 2.313 ± 0.042	2.648GlyAsn: 2.648 ± 0.047	2.239GlyPro: 2.239 ± 0.043	3.42GlyGln: 3.42 ± 0.051	4.382GlyArg: 4.382 ± 0.051	4.915GlySer: 4.915 ± 0.076	3.817GlyThr: 3.817 ± 0.07	6.006GlyVal: 6.006 ± 0.065	1.5GlyTrp: 1.5 ± 0.034	2.598GlyTyr: 2.598 ± 0.046	0.001GlyXaa: 0.001 ± 0.001
His	2.027HisAla: 2.027 ± 0.041	0.294HisCys: 0.294 ± 0.016	1.135HisAsp: 1.135 ± 0.027	0.963HisGlu: 0.963 ± 0.023	0.972HisPhe: 0.972 ± 0.031	1.811HisGly: 1.811 ± 0.036	0.727HisHis: 0.727 ± 0.024	1.108HisIle: 1.108 ± 0.029	0.606HisLys: 0.606 ± 0.022	2.209HisLeu: 2.209 ± 0.047	0.434HisMet: 0.434 ± 0.021	0.745HisAsn: 0.745 ± 0.023	1.374HisPro: 1.374 ± 0.034	1.286HisGln: 1.286 ± 0.033	1.346HisArg: 1.346 ± 0.035	1.224HisSer: 1.224 ± 0.031	1.033HisThr: 1.033 ± 0.025	1.14HisVal: 1.14 ± 0.032	0.415HisTrp: 0.415 ± 0.017	0.873HisTyr: 0.873 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.439IleAla: 6.439 ± 0.072	0.58IleCys: 0.58 ± 0.02	3.31IleAsp: 3.31 ± 0.054	2.9IleGlu: 2.9 ± 0.053	1.746IlePhe: 1.746 ± 0.041	4.515IleGly: 4.515 ± 0.062	1.06IleHis: 1.06 ± 0.031	2.626IleIle: 2.626 ± 0.047	1.917IleLys: 1.917 ± 0.04	4.428IleLeu: 4.428 ± 0.07	1.058IleMet: 1.058 ± 0.033	2.269IleAsn: 2.269 ± 0.041	2.401IlePro: 2.401 ± 0.041	1.52IleGln: 1.52 ± 0.035	2.866IleArg: 2.866 ± 0.046	3.527IleSer: 3.527 ± 0.056	3.172IleThr: 3.172 ± 0.057	3.599IleVal: 3.599 ± 0.049	0.649IleTrp: 0.649 ± 0.027	1.332IleTyr: 1.332 ± 0.031	0.001IleXaa: 0.001 ± 0.001
Lys	3.725LysAla: 3.725 ± 0.062	0.193LysCys: 0.193 ± 0.013	1.66LysAsp: 1.66 ± 0.037	1.897LysGlu: 1.897 ± 0.04	0.953LysPhe: 0.953 ± 0.025	2.537LysGly: 2.537 ± 0.047	0.678LysHis: 0.678 ± 0.021	1.987LysIle: 1.987 ± 0.042	1.826LysLys: 1.826 ± 0.044	3.605LysLeu: 3.605 ± 0.063	1.019LysMet: 1.019 ± 0.03	1.423LysAsn: 1.423 ± 0.033	1.882LysPro: 1.882 ± 0.036	1.742LysGln: 1.742 ± 0.038	2.243LysArg: 2.243 ± 0.043	2.103LysSer: 2.103 ± 0.044	2.178LysThr: 2.178 ± 0.046	2.537LysVal: 2.537 ± 0.049	0.372LysTrp: 0.372 ± 0.019	0.916LysTyr: 0.916 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	13.338LeuAla: 13.338 ± 0.137	1.285LeuCys: 1.285 ± 0.032	5.44LeuAsp: 5.44 ± 0.061	5.202LeuGlu: 5.202 ± 0.07	4.244LeuPhe: 4.244 ± 0.071	7.463LeuGly: 7.463 ± 0.075	2.365LeuHis: 2.365 ± 0.037	5.66LeuIle: 5.66 ± 0.075	4.077LeuLys: 4.077 ± 0.056	13.936LeuLeu: 13.936 ± 0.215	2.921LeuMet: 2.921 ± 0.056	4.209LeuAsn: 4.209 ± 0.05	6.257LeuPro: 6.257 ± 0.075	5.154LeuGln: 5.154 ± 0.078	6.712LeuArg: 6.712 ± 0.091	7.887LeuSer: 7.887 ± 0.082	6.79LeuThr: 6.79 ± 0.076	7.379LeuVal: 7.379 ± 0.099	1.515LeuTrp: 1.515 ± 0.039	2.511LeuTyr: 2.511 ± 0.043	0.001LeuXaa: 0.001 ± 0.001
Met	2.854MetAla: 2.854 ± 0.043	0.161MetCys: 0.161 ± 0.01	1.038MetAsp: 1.038 ± 0.022	1.008MetGlu: 1.008 ± 0.027	0.763MetPhe: 0.763 ± 0.025	1.622MetGly: 1.622 ± 0.036	0.488MetHis: 0.488 ± 0.017	1.328MetIle: 1.328 ± 0.033	1.22MetLys: 1.22 ± 0.029	3.031MetLeu: 3.031 ± 0.052	0.759MetMet: 0.759 ± 0.025	0.979MetAsn: 0.979 ± 0.025	1.246MetPro: 1.246 ± 0.03	1.217MetGln: 1.217 ± 0.033	1.402MetArg: 1.402 ± 0.031	1.829MetSer: 1.829 ± 0.037	1.676MetThr: 1.676 ± 0.036	1.79MetVal: 1.79 ± 0.033	0.228MetTrp: 0.228 ± 0.013	0.41MetTyr: 0.41 ± 0.018	0.001MetXaa: 0.001 ± 0.001
Asn	3.515AsnAla: 3.515 ± 0.06	0.281AsnCys: 0.281 ± 0.016	1.876AsnAsp: 1.876 ± 0.037	1.531AsnGlu: 1.531 ± 0.035	1.204AsnPhe: 1.204 ± 0.032	3.062AsnGly: 3.062 ± 0.054	0.739AsnHis: 0.739 ± 0.023	1.855AsnIle: 1.855 ± 0.041	1.192AsnLys: 1.192 ± 0.035	3.293AsnLeu: 3.293 ± 0.045	0.706AsnMet: 0.706 ± 0.021	1.357AsnAsn: 1.357 ± 0.038	2.04AsnPro: 2.04 ± 0.043	1.524AsnGln: 1.524 ± 0.04	2.007AsnArg: 2.007 ± 0.039	1.902AsnSer: 1.902 ± 0.04	1.756AsnThr: 1.756 ± 0.037	2.312AsnVal: 2.312 ± 0.042	0.533AsnTrp: 0.533 ± 0.022	1.018AsnTyr: 1.018 ± 0.034	0.001AsnXaa: 0.001 ± 0.001
Pro	5.666ProAla: 5.666 ± 0.081	0.339ProCys: 0.339 ± 0.016	2.806ProAsp: 2.806 ± 0.046	3.056ProGlu: 3.056 ± 0.047	1.738ProPhe: 1.738 ± 0.039	3.9ProGly: 3.9 ± 0.048	1.057ProHis: 1.057 ± 0.028	1.628ProIle: 1.628 ± 0.038	1.316ProLys: 1.316 ± 0.036	5.816ProLeu: 5.816 ± 0.085	1.021ProMet: 1.021 ± 0.027	1.094ProAsn: 1.094 ± 0.031	1.923ProPro: 1.923 ± 0.045	2.75ProGln: 2.75 ± 0.049	2.207ProArg: 2.207 ± 0.042	2.133ProSer: 2.133 ± 0.041	1.994ProThr: 1.994 ± 0.042	4.007ProVal: 4.007 ± 0.058	0.797ProTrp: 0.797 ± 0.025	1.133ProTyr: 1.133 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	5.448GlnAla: 5.448 ± 0.074	0.329GlnCys: 0.329 ± 0.016	1.89GlnAsp: 1.89 ± 0.043	1.954GlnGlu: 1.954 ± 0.037	1.505GlnPhe: 1.505 ± 0.034	3.457GlnGly: 3.457 ± 0.048	1.365GlnHis: 1.365 ± 0.032	2.263GlnIle: 2.263 ± 0.043	1.563GlnLys: 1.563 ± 0.035	5.912GlnLeu: 5.912 ± 0.075	1.219GlnMet: 1.219 ± 0.033	1.324GlnAsn: 1.324 ± 0.032	2.804GlnPro: 2.804 ± 0.053	4.568GlnGln: 4.568 ± 0.101	3.842GlnArg: 3.842 ± 0.061	2.4GlnSer: 2.4 ± 0.045	2.152GlnThr: 2.152 ± 0.042	3.352GlnVal: 3.352 ± 0.054	0.729GlnTrp: 0.729 ± 0.027	1.086GlnTyr: 1.086 ± 0.028	0.001GlnXaa: 0.001 ± 0.001
Arg	5.326ArgAla: 5.326 ± 0.065	0.632ArgCys: 0.632 ± 0.025	3.17ArgAsp: 3.17 ± 0.044	3.794ArgGlu: 3.794 ± 0.054	2.71ArgPhe: 2.71 ± 0.051	3.813ArgGly: 3.813 ± 0.058	1.694ArgHis: 1.694 ± 0.04	3.276ArgIle: 3.276 ± 0.046	1.997ArgLys: 1.997 ± 0.042	7.548ArgLeu: 7.548 ± 0.1	1.56ArgMet: 1.56 ± 0.033	1.984ArgAsn: 1.984 ± 0.049	2.609ArgPro: 2.609 ± 0.049	3.724ArgGln: 3.724 ± 0.063	3.975ArgArg: 3.975 ± 0.072	3.163ArgSer: 3.163 ± 0.055	2.507ArgThr: 2.507 ± 0.045	4.229ArgVal: 4.229 ± 0.061	1.125ArgTrp: 1.125 ± 0.031	2.276ArgTyr: 2.276 ± 0.043	0.001ArgXaa: 0.001 ± 0.001
Ser	6.562SerAla: 6.562 ± 0.082	0.559SerCys: 0.559 ± 0.019	3.206SerAsp: 3.206 ± 0.048	3.22SerGlu: 3.22 ± 0.051	2.223SerPhe: 2.223 ± 0.044	6.021SerGly: 6.021 ± 0.083	1.445SerHis: 1.445 ± 0.032	2.612SerIle: 2.612 ± 0.048	1.784SerLys: 1.784 ± 0.043	7.17SerLeu: 7.17 ± 0.085	1.364SerMet: 1.364 ± 0.032	1.831SerAsn: 1.831 ± 0.042	2.781SerPro: 2.781 ± 0.048	2.745SerGln: 2.745 ± 0.055	3.737SerArg: 3.737 ± 0.056	3.671SerSer: 3.671 ± 0.06	2.911SerThr: 2.911 ± 0.047	4.457SerVal: 4.457 ± 0.064	1.075SerTrp: 1.075 ± 0.031	1.636SerTyr: 1.636 ± 0.033	0.001SerXaa: 0.001 ± 0.001
Thr	5.471ThrAla: 5.471 ± 0.084	0.42ThrCys: 0.42 ± 0.016	2.57ThrAsp: 2.57 ± 0.046	2.363ThrGlu: 2.363 ± 0.041	1.965ThrPhe: 1.965 ± 0.045	4.379ThrGly: 4.379 ± 0.057	1.064ThrHis: 1.064 ± 0.027	2.53ThrIle: 2.53 ± 0.045	1.197ThrLys: 1.197 ± 0.033	7.944ThrLeu: 7.944 ± 0.106	0.894ThrMet: 0.894 ± 0.027	1.305ThrAsn: 1.305 ± 0.034	3.274ThrPro: 3.274 ± 0.048	1.987ThrGln: 1.987 ± 0.041	3.345ThrArg: 3.345 ± 0.047	2.852ThrSer: 2.852 ± 0.053	2.83ThrThr: 2.83 ± 0.056	3.835ThrVal: 3.835 ± 0.069	0.679ThrTrp: 0.679 ± 0.022	1.093ThrTyr: 1.093 ± 0.029	0.001ThrXaa: 0.001 ± 0.001
Val	7.946ValAla: 7.946 ± 0.08	0.704ValCys: 0.704 ± 0.023	3.56ValAsp: 3.56 ± 0.055	3.749ValGlu: 3.749 ± 0.058	2.521ValPhe: 2.521 ± 0.044	4.7ValGly: 4.7 ± 0.067	1.187ValHis: 1.187 ± 0.029	4.018ValIle: 4.018 ± 0.056	2.717ValLys: 2.717 ± 0.042	7.561ValLeu: 7.561 ± 0.088	2.15ValMet: 2.15 ± 0.039	2.608ValAsn: 2.608 ± 0.047	3.058ValPro: 3.058 ± 0.051	2.692ValGln: 2.692 ± 0.051	3.828ValArg: 3.828 ± 0.055	5.056ValSer: 5.056 ± 0.069	4.417ValThr: 4.417 ± 0.074	5.506ValVal: 5.506 ± 0.073	0.96ValTrp: 0.96 ± 0.025	1.548ValTyr: 1.548 ± 0.037	0.001ValXaa: 0.001 ± 0.001
Trp	1.032TrpAla: 1.032 ± 0.029	0.175TrpCys: 0.175 ± 0.012	0.622TrpAsp: 0.622 ± 0.022	0.506TrpGlu: 0.506 ± 0.021	0.676TrpPhe: 0.676 ± 0.022	0.948TrpGly: 0.948 ± 0.028	0.448TrpHis: 0.448 ± 0.02	0.655TrpIle: 0.655 ± 0.024	0.436TrpLys: 0.436 ± 0.017	2.577TrpLeu: 2.577 ± 0.052	0.41TrpMet: 0.41 ± 0.018	0.472TrpAsn: 0.472 ± 0.019	0.747TrpPro: 0.747 ± 0.025	1.432TrpGln: 1.432 ± 0.04	1.141TrpArg: 1.141 ± 0.028	0.859TrpSer: 0.859 ± 0.026	0.561TrpThr: 0.561 ± 0.021	0.96TrpVal: 0.96 ± 0.027	0.244TrpTrp: 0.244 ± 0.013	0.45TrpTyr: 0.45 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.469TyrAla: 2.469 ± 0.039	0.333TyrCys: 0.333 ± 0.016	1.445TyrAsp: 1.445 ± 0.038	1.069TyrGlu: 1.069 ± 0.028	1.041TyrPhe: 1.041 ± 0.031	2.124TyrGly: 2.124 ± 0.04	0.679TyrHis: 0.679 ± 0.025	1.137TyrIle: 1.137 ± 0.029	0.755TyrLys: 0.755 ± 0.024	2.976TyrLeu: 2.976 ± 0.05	0.52TyrMet: 0.52 ± 0.017	0.888TyrAsn: 0.888 ± 0.03	1.366TyrPro: 1.366 ± 0.035	1.69TyrGln: 1.69 ± 0.037	1.98TyrArg: 1.98 ± 0.037	1.576TyrSer: 1.576 ± 0.034	1.295TyrThr: 1.295 ± 0.035	1.579TyrVal: 1.579 ± 0.037	0.432TyrTrp: 0.432 ± 0.018	0.818TyrTyr: 0.818 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.002	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4431 proteins (1423473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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