Aureibaculum marinum
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3096 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4NWT2|A0A3N4NWT2_9FLAO Uncharacterized protein OS=Aureibaculum marinum OX=2487930 GN=EGM88_09820 PE=4 SV=1
MM1 pKa = 7.62 IKK3 pKa = 10.14 QFIKK7 pKa = 10.8 LSTLCFVILLGFTSCDD23 pKa = 3.52 SDD25 pKa = 4.09 DD26 pKa = 4.37 QDD28 pKa = 3.67 PVMPKK33 pKa = 10.22 GDD35 pKa = 3.72 YY36 pKa = 9.26 EE37 pKa = 3.93 NGYY40 pKa = 9.98 FVVNEE45 pKa = 4.41 GNFGTPNGSVTFIDD59 pKa = 5.44 DD60 pKa = 4.72 NLLQEE65 pKa = 4.37 NNEE68 pKa = 3.88 IFQSVNSTTLGDD80 pKa = 3.58 VVQYY84 pKa = 9.46 ITFEE88 pKa = 4.33 DD89 pKa = 4.41 DD90 pKa = 3.26 YY91 pKa = 11.95 GFIVVNNSNKK101 pKa = 9.2 IEE103 pKa = 4.15 VVNRR107 pKa = 11.84 YY108 pKa = 6.26 TFEE111 pKa = 4.02 SVATITEE118 pKa = 4.04 NLQLPRR124 pKa = 11.84 YY125 pKa = 9.44 AVVEE129 pKa = 3.95 NGKK132 pKa = 10.7 LYY134 pKa = 9.18 VTNSGTNSVEE144 pKa = 3.9 VFDD147 pKa = 5.88 ANTFAYY153 pKa = 9.6 INSIDD158 pKa = 3.31 IDD160 pKa = 3.81 KK161 pKa = 9.81 TVEE164 pKa = 4.2 EE165 pKa = 4.31 IVEE168 pKa = 4.36 EE169 pKa = 4.0 NDD171 pKa = 3.31 LLYY174 pKa = 11.58 VMNASFGFGNEE185 pKa = 3.17 ITVINTQSDD194 pKa = 4.39 TVVKK198 pKa = 9.62 TITVGDD204 pKa = 3.95 GLNSIEE210 pKa = 4.24 VEE212 pKa = 4.12 DD213 pKa = 5.56 DD214 pKa = 2.78 ILYY217 pKa = 10.3 ALHH220 pKa = 6.6 SAGITKK226 pKa = 10.17 VNTATNEE233 pKa = 4.21 VIGEE237 pKa = 4.23 VPFEE241 pKa = 5.51 DD242 pKa = 4.45 GLLMASKK249 pKa = 11.11 LEE251 pKa = 3.83 VDD253 pKa = 3.68 DD254 pKa = 4.61 NYY256 pKa = 10.62 IYY258 pKa = 10.61 FLSGSKK264 pKa = 9.67 IFKK267 pKa = 9.25 YY268 pKa = 10.81 NKK270 pKa = 10.04 DD271 pKa = 3.41 VTSLANTEE279 pKa = 4.39 LVDD282 pKa = 3.92 TQVEE286 pKa = 4.15 DD287 pKa = 3.8 ASWYY291 pKa = 9.39 IGYY294 pKa = 9.7 GFNVIDD300 pKa = 3.89 NKK302 pKa = 10.85 LFYY305 pKa = 10.32 TDD307 pKa = 3.07 VKK309 pKa = 10.85 GFSEE313 pKa = 4.02 NSEE316 pKa = 4.12 VKK318 pKa = 10.75 VYY320 pKa = 10.98 DD321 pKa = 4.07 LNGDD325 pKa = 4.05 LLTTFNAGIGANGVYY340 pKa = 10.58 EE341 pKa = 4.1 NDD343 pKa = 3.3
Molecular weight: 38.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.579
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.91
Patrickios 0.973
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A3N4NTB4|A0A3N4NTB4_9FLAO Thioredoxin OS=Aureibaculum marinum OX=2487930 GN=EGM88_03280 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 10.15 RR11 pKa = 11.84 KK12 pKa = 9.6 RR13 pKa = 11.84 KK14 pKa = 8.29 NKK16 pKa = 9.34 HH17 pKa = 4.05 GFRR20 pKa = 11.84 EE21 pKa = 4.13 RR22 pKa = 11.84 MSSVNGRR29 pKa = 11.84 KK30 pKa = 9.11 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.76 GRR40 pKa = 11.84 KK41 pKa = 8.18 RR42 pKa = 11.84 LSVSSDD48 pKa = 3.33 PSPKK52 pKa = 10.05 KK53 pKa = 10.54
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3096
0
3096
1089046
24
2458
351.8
39.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.867 ± 0.04
0.661 ± 0.012
5.578 ± 0.05
6.445 ± 0.039
5.122 ± 0.038
6.275 ± 0.053
1.7 ± 0.019
8.435 ± 0.043
8.57 ± 0.064
9.23 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.019
6.746 ± 0.039
3.292 ± 0.023
3.176 ± 0.025
3.164 ± 0.024
6.506 ± 0.03
5.816 ± 0.041
6.062 ± 0.041
1.054 ± 0.017
4.26 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here