Chrysochromulina parva virus
Average proteome isoelectric point is 7.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6GRC3|A0A4Y6GRC3_9PHYC Transcriptional regulatory protein OS=Chrysochromulina parva virus OX=2565304 PE=4 SV=1
MM1 pKa = 7.45 SFLFKK6 pKa = 10.89 FIDD9 pKa = 3.48 SGFLLTFGLILLISGGIMLYY29 pKa = 10.02 CYY31 pKa = 10.23 RR32 pKa = 11.84 RR33 pKa = 11.84 LNLLEE38 pKa = 4.29 KK39 pKa = 10.71 SVIEE43 pKa = 4.44 HH44 pKa = 6.49 GKK46 pKa = 9.46 ILQNFIMNYY55 pKa = 8.65 NIQMQHH61 pKa = 6.11 FSLLNKK67 pKa = 10.48 SMTNSSSNTNATNVEE82 pKa = 4.05 NSRR85 pKa = 11.84 AEE87 pKa = 3.88 YY88 pKa = 11.19 VEE90 pKa = 3.52 FDD92 pKa = 3.34 KK93 pKa = 11.45 VKK95 pKa = 10.58 KK96 pKa = 10.31 INLGDD101 pKa = 4.27 KK102 pKa = 10.46 ISVSDD107 pKa = 5.38 DD108 pKa = 3.12 EE109 pKa = 6.62 DD110 pKa = 3.79 EE111 pKa = 6.31 ADD113 pKa = 6.24 DD114 pKa = 5.73 EE115 pKa = 6.65 DD116 pKa = 6.93 DD117 pKa = 6.47 DD118 pKa = 7.52 DD119 pKa = 7.51 DD120 pKa = 7.29 DD121 pKa = 7.39 DD122 pKa = 6.44 EE123 pKa = 8.1 DD124 pKa = 6.79 DD125 pKa = 6.45 DD126 pKa = 7.45 DD127 pKa = 7.49 DD128 pKa = 6.96 DD129 pKa = 7.33 DD130 pKa = 4.85 EE131 pKa = 7.44 DD132 pKa = 4.39 EE133 pKa = 6.61 DD134 pKa = 4.98 EE135 pKa = 6.38 DD136 pKa = 5.7 EE137 pKa = 5.38 DD138 pKa = 6.48 DD139 pKa = 4.22 EE140 pKa = 7.24 DD141 pKa = 5.22 EE142 pKa = 6.73 DD143 pKa = 5.67 EE144 pKa = 6.56 DD145 pKa = 6.54 DD146 pKa = 6.53 DD147 pKa = 6.55 DD148 pKa = 7.28 DD149 pKa = 5.38 EE150 pKa = 7.51 DD151 pKa = 5.02 EE152 pKa = 6.75 DD153 pKa = 4.02 EE154 pKa = 5.22 DD155 pKa = 4.73 KK156 pKa = 11.82 NNTEE160 pKa = 4.23 SLKK163 pKa = 10.57 ISNNKK168 pKa = 10.04 LEE170 pKa = 4.51 DD171 pKa = 4.0 LEE173 pKa = 5.01 DD174 pKa = 5.08 LEE176 pKa = 5.57 DD177 pKa = 5.76 LEE179 pKa = 5.77 DD180 pKa = 5.65 LEE182 pKa = 5.0 DD183 pKa = 3.85 LEE185 pKa = 5.76 KK186 pKa = 10.61 IEE188 pKa = 6.3 LSDD191 pKa = 3.53 QTISSIDD198 pKa = 3.6 DD199 pKa = 3.23 EE200 pKa = 4.89 TFLKK204 pKa = 10.59 NLPINLDD211 pKa = 3.63 SFTLDD216 pKa = 3.3 NTNNEE221 pKa = 4.0 PKK223 pKa = 10.17 IINLEE228 pKa = 4.27 NIQDD232 pKa = 4.08 SNTKK236 pKa = 8.67 TGEE239 pKa = 3.87 RR240 pKa = 11.84 KK241 pKa = 9.86 NYY243 pKa = 10.82 SKK245 pKa = 10.71 MKK247 pKa = 10.4 VDD249 pKa = 3.88 DD250 pKa = 4.5 LKK252 pKa = 10.67 TLVVTKK258 pKa = 10.69 NLTDD262 pKa = 3.46 NEE264 pKa = 4.52 SAQKK268 pKa = 9.41 MKK270 pKa = 10.83 KK271 pKa = 9.61 SDD273 pKa = 3.91 LVKK276 pKa = 10.71 LLQKK280 pKa = 10.32 EE281 pKa = 4.16
Molecular weight: 32.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.287
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 3.783
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A4Y6GT19|A0A4Y6GT19_9PHYC Uncharacterized protein OS=Chrysochromulina parva virus OX=2565304 PE=4 SV=1
MM1 pKa = 7.33 SRR3 pKa = 11.84 RR4 pKa = 11.84 STRR7 pKa = 11.84 VVSVSSDD14 pKa = 2.86 SDD16 pKa = 3.28 SRR18 pKa = 11.84 TRR20 pKa = 11.84 SKK22 pKa = 11.16 SKK24 pKa = 10.67 NSTLFRR30 pKa = 11.84 AYY32 pKa = 9.67 KK33 pKa = 10.46 SVFGPSKK40 pKa = 10.86 SKK42 pKa = 10.27 LASIARR48 pKa = 11.84 FRR50 pKa = 11.84 SVIEE54 pKa = 4.43 GCDD57 pKa = 3.21 EE58 pKa = 4.0 FLNNITDD65 pKa = 3.66 KK66 pKa = 11.12 NIPDD70 pKa = 4.77 LYY72 pKa = 10.83 GQIPKK77 pKa = 9.92 ILNIRR82 pKa = 11.84 NEE84 pKa = 4.23 NYY86 pKa = 10.18 DD87 pKa = 3.71 KK88 pKa = 11.48 LSDD91 pKa = 3.69 ATRR94 pKa = 11.84 LTAEE98 pKa = 5.44 DD99 pKa = 3.25 IAANQAIHH107 pKa = 6.83 AKK109 pKa = 9.34 IKK111 pKa = 10.43 NYY113 pKa = 9.46 HH114 pKa = 5.79 ANHH117 pKa = 6.89 RR118 pKa = 11.84 SRR120 pKa = 11.84 YY121 pKa = 7.24 TSYY124 pKa = 11.16 NYY126 pKa = 10.84 NLAGTRR132 pKa = 11.84 KK133 pKa = 8.6 RR134 pKa = 11.84 RR135 pKa = 11.84 KK136 pKa = 8.38 HH137 pKa = 6.05 RR138 pKa = 11.84 KK139 pKa = 7.85 HH140 pKa = 6.43 KK141 pKa = 10.41 KK142 pKa = 8.47 HH143 pKa = 6.15
Molecular weight: 16.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.853
IPC_protein 10.452
Toseland 10.716
ProMoST 10.335
Dawson 10.818
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.213
Grimsley 10.862
Solomon 10.891
Lehninger 10.862
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.73
Patrickios 10.921
IPC_peptide 10.891
IPC2_peptide 9.224
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
14096
100
2116
313.2
36.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.483 ± 0.364
1.674 ± 0.174
5.314 ± 0.372
6.881 ± 0.539
4.682 ± 0.273
3.497 ± 0.336
1.766 ± 0.202
9.499 ± 0.41
10.577 ± 0.534
10.187 ± 0.83
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.717 ± 0.153
9.776 ± 0.474
2.774 ± 0.36
2.831 ± 0.256
2.887 ± 0.324
7.513 ± 0.72
5.214 ± 0.369
4.072 ± 0.255
0.532 ± 0.093
5.122 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here