Eubacterium ruminantium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4Q7U3|A0A1T4Q7U3_9FIRM GTP cyclohydrolase 1 OS=Eubacterium ruminantium OX=42322 GN=folE PE=3 SV=1
MM1 pKa = 7.32VIGYY5 pKa = 8.15TDD7 pKa = 3.46EE8 pKa = 4.44NEE10 pKa = 4.26CNFVIKK16 pKa = 10.62FDD18 pKa = 4.97DD19 pKa = 3.54EE20 pKa = 4.46TKK22 pKa = 10.69ADD24 pKa = 3.9EE25 pKa = 4.32VKK27 pKa = 10.57KK28 pKa = 10.62AIQIGIGAWYY38 pKa = 8.6EE39 pKa = 3.82AAHH42 pKa = 7.54DD43 pKa = 4.92DD44 pKa = 4.21IQPNEE49 pKa = 4.09YY50 pKa = 8.87WDD52 pKa = 3.94KK53 pKa = 11.3EE54 pKa = 4.41DD55 pKa = 4.47IEE57 pKa = 4.88GFYY60 pKa = 11.06DD61 pKa = 3.6CGYY64 pKa = 11.0AEE66 pKa = 4.46PTMEE70 pKa = 5.69LLDD73 pKa = 3.37MWGIKK78 pKa = 10.13HH79 pKa = 5.76EE80 pKa = 4.4VVDD83 pKa = 5.72IEE85 pKa = 4.77NDD87 pKa = 3.44DD88 pKa = 4.06NGNVICDD95 pKa = 3.69EE96 pKa = 4.28LVV98 pKa = 2.91

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4N3L3|A0A1T4N3L3_9FIRM Beta sliding clamp OS=Eubacterium ruminantium OX=42322 GN=SAMN02745110_01448 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.33VHH16 pKa = 6.24GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.98VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84KK40 pKa = 9.22SISAA44 pKa = 3.61

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2481

0

2481

845742

39

3747

340.9

38.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.452 ± 0.048

1.269 ± 0.017

6.774 ± 0.042

7.722 ± 0.053

4.26 ± 0.033

6.766 ± 0.048

1.513 ± 0.02

8.347 ± 0.047

8.127 ± 0.047

7.921 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.027

5.304 ± 0.043

2.905 ± 0.029

2.215 ± 0.027

3.996 ± 0.034

6.309 ± 0.041

5.131 ± 0.047

6.754 ± 0.033

0.782 ± 0.015

4.545 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski