Amino acid dipepetide frequency for Eubacterium ruminantium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.328AlaAla: 5.328 ± 0.116	0.787AlaCys: 0.787 ± 0.031	4.316AlaAsp: 4.316 ± 0.079	5.294AlaGlu: 5.294 ± 0.11	2.874AlaPhe: 2.874 ± 0.064	4.948AlaGly: 4.948 ± 0.082	0.822AlaHis: 0.822 ± 0.031	4.863AlaIle: 4.863 ± 0.084	4.866AlaLys: 4.866 ± 0.097	5.414AlaLeu: 5.414 ± 0.09	1.793AlaMet: 1.793 ± 0.056	2.607AlaAsn: 2.607 ± 0.066	1.622AlaPro: 1.622 ± 0.052	1.387AlaGln: 1.387 ± 0.051	2.475AlaArg: 2.475 ± 0.059	3.887AlaSer: 3.887 ± 0.078	3.022AlaThr: 3.022 ± 0.078	5.227AlaVal: 5.227 ± 0.091	0.427AlaTrp: 0.427 ± 0.021	2.565AlaTyr: 2.565 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.73CysAla: 0.73 ± 0.033	0.231CysCys: 0.231 ± 0.015	0.843CysAsp: 0.843 ± 0.031	0.869CysGlu: 0.869 ± 0.04	0.61CysPhe: 0.61 ± 0.027	1.185CysGly: 1.185 ± 0.042	0.246CysHis: 0.246 ± 0.018	1.126CysIle: 1.126 ± 0.035	0.879CysLys: 0.879 ± 0.04	0.923CysLeu: 0.923 ± 0.036	0.359CysMet: 0.359 ± 0.022	0.605CysAsn: 0.605 ± 0.031	0.511CysPro: 0.511 ± 0.028	0.24CysGln: 0.24 ± 0.016	0.538CysArg: 0.538 ± 0.026	0.78CysSer: 0.78 ± 0.032	0.562CysThr: 0.562 ± 0.027	0.79CysVal: 0.79 ± 0.031	0.102CysTrp: 0.102 ± 0.011	0.565CysTyr: 0.565 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.053AspAla: 4.053 ± 0.077	0.667AspCys: 0.667 ± 0.031	4.717AspAsp: 4.717 ± 0.112	6.121AspGlu: 6.121 ± 0.104	3.058AspPhe: 3.058 ± 0.061	4.506AspGly: 4.506 ± 0.098	0.957AspHis: 0.957 ± 0.038	6.443AspIle: 6.443 ± 0.095	6.115AspLys: 6.115 ± 0.094	4.381AspLeu: 4.381 ± 0.077	2.041AspMet: 2.041 ± 0.057	3.567AspAsn: 3.567 ± 0.073	1.797AspPro: 1.797 ± 0.051	1.25AspGln: 1.25 ± 0.042	2.526AspArg: 2.526 ± 0.067	4.047AspSer: 4.047 ± 0.094	3.189AspThr: 3.189 ± 0.063	4.278AspVal: 4.278 ± 0.076	0.536AspTrp: 0.536 ± 0.028	3.496AspTyr: 3.496 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	5.231GluAla: 5.231 ± 0.102	0.804GluCys: 0.804 ± 0.036	4.888GluAsp: 4.888 ± 0.1	7.507GluGlu: 7.507 ± 0.143	2.937GluPhe: 2.937 ± 0.065	4.412GluGly: 4.412 ± 0.082	1.077GluHis: 1.077 ± 0.037	6.235GluIle: 6.235 ± 0.094	7.451GluLys: 7.451 ± 0.126	6.229GluLeu: 6.229 ± 0.097	2.451GluMet: 2.451 ± 0.06	4.665GluAsn: 4.665 ± 0.08	1.603GluPro: 1.603 ± 0.044	1.9GluGln: 1.9 ± 0.048	3.236GluArg: 3.236 ± 0.065	3.827GluSer: 3.827 ± 0.074	3.619GluThr: 3.619 ± 0.071	4.797GluVal: 4.797 ± 0.082	0.624GluTrp: 0.624 ± 0.028	3.73GluTyr: 3.73 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	2.679PheAla: 2.679 ± 0.059	0.635PheCys: 0.635 ± 0.031	3.188PheAsp: 3.188 ± 0.069	2.811PheGlu: 2.811 ± 0.058	1.865PhePhe: 1.865 ± 0.059	3.085PheGly: 3.085 ± 0.067	0.669PheHis: 0.669 ± 0.027	3.785PheIle: 3.785 ± 0.092	2.861PheLys: 2.861 ± 0.062	3.393PheLeu: 3.393 ± 0.067	1.311PheMet: 1.311 ± 0.047	2.041PheAsn: 2.041 ± 0.053	1.31PhePro: 1.31 ± 0.043	0.79PheGln: 0.79 ± 0.026	1.573PheArg: 1.573 ± 0.046	3.043PheSer: 3.043 ± 0.06	2.372PheThr: 2.372 ± 0.057	2.931PheVal: 2.931 ± 0.061	0.365PheTrp: 0.365 ± 0.024	1.888PheTyr: 1.888 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	4.167GlyAla: 4.167 ± 0.094	1.006GlyCys: 1.006 ± 0.037	3.975GlyAsp: 3.975 ± 0.078	4.497GlyGlu: 4.497 ± 0.083	3.217GlyPhe: 3.217 ± 0.068	4.52GlyGly: 4.52 ± 0.119	1.186GlyHis: 1.186 ± 0.035	5.964GlyIle: 5.964 ± 0.083	5.762GlyLys: 5.762 ± 0.095	5.302GlyLeu: 5.302 ± 0.085	2.121GlyMet: 2.121 ± 0.057	3.655GlyAsn: 3.655 ± 0.097	1.182GlyPro: 1.182 ± 0.042	1.561GlyGln: 1.561 ± 0.057	2.919GlyArg: 2.919 ± 0.07	4.002GlySer: 4.002 ± 0.089	3.78GlyThr: 3.78 ± 0.085	4.65GlyVal: 4.65 ± 0.089	0.679GlyTrp: 0.679 ± 0.037	3.509GlyTyr: 3.509 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	0.787HisAla: 0.787 ± 0.037	0.214HisCys: 0.214 ± 0.019	1.019HisAsp: 1.019 ± 0.043	1.007HisGlu: 1.007 ± 0.035	0.81HisPhe: 0.81 ± 0.028	1.076HisGly: 1.076 ± 0.037	0.346HisHis: 0.346 ± 0.039	1.286HisIle: 1.286 ± 0.046	1.078HisLys: 1.078 ± 0.03	1.159HisLeu: 1.159 ± 0.037	0.428HisMet: 0.428 ± 0.022	0.774HisAsn: 0.774 ± 0.034	0.661HisPro: 0.661 ± 0.029	0.346HisGln: 0.346 ± 0.021	0.625HisArg: 0.625 ± 0.03	0.992HisSer: 0.992 ± 0.037	0.783HisThr: 0.783 ± 0.031	0.933HisVal: 0.933 ± 0.034	0.111HisTrp: 0.111 ± 0.011	0.688HisTyr: 0.688 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.265IleAla: 5.265 ± 0.088	1.336IleCys: 1.336 ± 0.041	5.668IleAsp: 5.668 ± 0.078	5.809IleGlu: 5.809 ± 0.097	3.49IlePhe: 3.49 ± 0.09	5.143IleGly: 5.143 ± 0.086	1.223IleHis: 1.223 ± 0.045	7.213IleIle: 7.213 ± 0.127	6.404IleLys: 6.404 ± 0.099	6.775IleLeu: 6.775 ± 0.117	2.217IleMet: 2.217 ± 0.053	4.539IleAsn: 4.539 ± 0.079	3.066IlePro: 3.066 ± 0.062	1.642IleGln: 1.642 ± 0.054	3.514IleArg: 3.514 ± 0.074	6.146IleSer: 6.146 ± 0.1	4.344IleThr: 4.344 ± 0.097	5.35IleVal: 5.35 ± 0.087	0.579IleTrp: 0.579 ± 0.028	3.74IleTyr: 3.74 ± 0.074	0.0IleXaa: 0.0 ± 0.0
Lys	5.415LysAla: 5.415 ± 0.103	1.011LysCys: 1.011 ± 0.036	5.636LysAsp: 5.636 ± 0.073	7.183LysGlu: 7.183 ± 0.111	2.608LysPhe: 2.608 ± 0.056	4.655LysGly: 4.655 ± 0.08	1.106LysHis: 1.106 ± 0.031	6.078LysIle: 6.078 ± 0.085	7.991LysLys: 7.991 ± 0.125	6.328LysLeu: 6.328 ± 0.094	2.442LysMet: 2.442 ± 0.053	5.203LysAsn: 5.203 ± 0.092	2.095LysPro: 2.095 ± 0.052	1.797LysGln: 1.797 ± 0.046	3.168LysArg: 3.168 ± 0.071	4.459LysSer: 4.459 ± 0.077	4.192LysThr: 4.192 ± 0.073	5.337LysVal: 5.337 ± 0.086	0.66LysTrp: 0.66 ± 0.03	3.903LysTyr: 3.903 ± 0.074	0.0LysXaa: 0.0 ± 0.0
Leu	5.128LeuAla: 5.128 ± 0.086	1.049LeuCys: 1.049 ± 0.038	4.953LeuAsp: 4.953 ± 0.088	5.09LeuGlu: 5.09 ± 0.084	3.481LeuPhe: 3.481 ± 0.085	5.317LeuGly: 5.317 ± 0.088	1.249LeuHis: 1.249 ± 0.039	6.404LeuIle: 6.404 ± 0.106	6.387LeuLys: 6.387 ± 0.083	6.485LeuLeu: 6.485 ± 0.105	2.485LeuMet: 2.485 ± 0.057	3.871LeuAsn: 3.871 ± 0.079	2.757LeuPro: 2.757 ± 0.054	1.82LeuGln: 1.82 ± 0.048	3.496LeuArg: 3.496 ± 0.08	6.174LeuSer: 6.174 ± 0.089	4.241LeuThr: 4.241 ± 0.066	4.892LeuVal: 4.892 ± 0.09	0.569LeuTrp: 0.569 ± 0.026	3.364LeuTyr: 3.364 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	2.053MetAla: 2.053 ± 0.054	0.342MetCys: 0.342 ± 0.02	1.858MetAsp: 1.858 ± 0.053	1.875MetGlu: 1.875 ± 0.05	1.033MetPhe: 1.033 ± 0.041	1.852MetGly: 1.852 ± 0.042	0.432MetHis: 0.432 ± 0.026	2.53MetIle: 2.53 ± 0.064	2.574MetLys: 2.574 ± 0.064	2.484MetLeu: 2.484 ± 0.064	0.866MetMet: 0.866 ± 0.037	1.862MetAsn: 1.862 ± 0.048	1.007MetPro: 1.007 ± 0.035	0.731MetGln: 0.731 ± 0.032	1.153MetArg: 1.153 ± 0.037	1.902MetSer: 1.902 ± 0.044	1.583MetThr: 1.583 ± 0.043	1.745MetVal: 1.745 ± 0.056	0.197MetTrp: 0.197 ± 0.016	1.022MetTyr: 1.022 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	3.358AsnAla: 3.358 ± 0.058	0.586AsnCys: 0.586 ± 0.026	3.641AsnAsp: 3.641 ± 0.074	3.947AsnGlu: 3.947 ± 0.071	1.817AsnPhe: 1.817 ± 0.052	4.064AsnGly: 4.064 ± 0.107	0.767AsnHis: 0.767 ± 0.031	4.871AsnIle: 4.871 ± 0.094	4.222AsnLys: 4.222 ± 0.083	4.075AsnLeu: 4.075 ± 0.081	1.531AsnMet: 1.531 ± 0.051	3.01AsnAsn: 3.01 ± 0.082	2.005AsnPro: 2.005 ± 0.054	1.185AsnGln: 1.185 ± 0.046	1.978AsnArg: 1.978 ± 0.047	3.184AsnSer: 3.184 ± 0.071	2.634AsnThr: 2.634 ± 0.071	3.505AsnVal: 3.505 ± 0.072	0.375AsnTrp: 0.375 ± 0.021	2.286AsnTyr: 2.286 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	1.88ProAla: 1.88 ± 0.056	0.37ProCys: 0.37 ± 0.024	2.43ProAsp: 2.43 ± 0.062	3.077ProGlu: 3.077 ± 0.057	1.344ProPhe: 1.344 ± 0.037	2.048ProGly: 2.048 ± 0.053	0.452ProHis: 0.452 ± 0.023	2.036ProIle: 2.036 ± 0.051	1.95ProLys: 1.95 ± 0.049	2.1ProLeu: 2.1 ± 0.053	0.74ProMet: 0.74 ± 0.03	1.259ProAsn: 1.259 ± 0.046	0.534ProPro: 0.534 ± 0.027	0.7ProGln: 0.7 ± 0.033	0.88ProArg: 0.88 ± 0.032	1.662ProSer: 1.662 ± 0.044	1.482ProThr: 1.482 ± 0.05	2.528ProVal: 2.528 ± 0.063	0.252ProTrp: 0.252 ± 0.019	1.324ProTyr: 1.324 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	1.425GlnAla: 1.425 ± 0.051	0.259GlnCys: 0.259 ± 0.016	1.214GlnAsp: 1.214 ± 0.031	1.586GlnGlu: 1.586 ± 0.046	0.942GlnPhe: 0.942 ± 0.035	1.369GlnGly: 1.369 ± 0.049	0.356GlnHis: 0.356 ± 0.021	1.84GlnIle: 1.84 ± 0.046	1.891GlnLys: 1.891 ± 0.056	1.919GlnLeu: 1.919 ± 0.052	0.76GlnMet: 0.76 ± 0.031	1.348GlnAsn: 1.348 ± 0.051	0.666GlnPro: 0.666 ± 0.038	0.737GlnGln: 0.737 ± 0.049	0.964GlnArg: 0.964 ± 0.037	1.294GlnSer: 1.294 ± 0.04	1.044GlnThr: 1.044 ± 0.037	1.288GlnVal: 1.288 ± 0.04	0.194GlnTrp: 0.194 ± 0.016	1.054GlnTyr: 1.054 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.232ArgAla: 2.232 ± 0.057	0.486ArgCys: 0.486 ± 0.022	2.528ArgAsp: 2.528 ± 0.068	3.279ArgGlu: 3.279 ± 0.076	1.912ArgPhe: 1.912 ± 0.052	2.209ArgGly: 2.209 ± 0.051	0.685ArgHis: 0.685 ± 0.029	3.456ArgIle: 3.456 ± 0.073	3.493ArgLys: 3.493 ± 0.063	3.352ArgLeu: 3.352 ± 0.068	1.25ArgMet: 1.25 ± 0.039	2.187ArgAsn: 2.187 ± 0.057	1.084ArgPro: 1.084 ± 0.046	1.046ArgGln: 1.046 ± 0.038	2.034ArgArg: 2.034 ± 0.057	2.096ArgSer: 2.096 ± 0.056	1.949ArgThr: 1.949 ± 0.047	2.533ArgVal: 2.533 ± 0.06	0.325ArgTrp: 0.325 ± 0.019	1.823ArgTyr: 1.823 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.849SerAla: 3.849 ± 0.074	0.713SerCys: 0.713 ± 0.03	4.718SerAsp: 4.718 ± 0.094	4.866SerGlu: 4.866 ± 0.096	3.112SerPhe: 3.112 ± 0.074	5.411SerGly: 5.411 ± 0.112	0.981SerHis: 0.981 ± 0.039	4.958SerIle: 4.958 ± 0.084	4.53SerLys: 4.53 ± 0.07	5.095SerLeu: 5.095 ± 0.085	1.675SerMet: 1.675 ± 0.049	2.945SerAsn: 2.945 ± 0.065	1.646SerPro: 1.646 ± 0.039	1.392SerGln: 1.392 ± 0.054	2.37SerArg: 2.37 ± 0.057	4.158SerSer: 4.158 ± 0.104	2.949SerThr: 2.949 ± 0.066	4.423SerVal: 4.423 ± 0.079	0.453SerTrp: 0.453 ± 0.026	2.844SerTyr: 2.844 ± 0.066	0.0SerXaa: 0.0 ± 0.0
Thr	3.606ThrAla: 3.606 ± 0.091	0.516ThrCys: 0.516 ± 0.023	3.507ThrAsp: 3.507 ± 0.079	4.037ThrGlu: 4.037 ± 0.091	2.216ThrPhe: 2.216 ± 0.055	4.32ThrGly: 4.32 ± 0.077	0.76ThrHis: 0.76 ± 0.032	3.991ThrIle: 3.991 ± 0.072	3.312ThrLys: 3.312 ± 0.072	4.151ThrLeu: 4.151 ± 0.071	1.192ThrMet: 1.192 ± 0.044	2.289ThrAsn: 2.289 ± 0.061	1.798ThrPro: 1.798 ± 0.046	1.01ThrGln: 1.01 ± 0.037	1.729ThrArg: 1.729 ± 0.046	3.129ThrSer: 3.129 ± 0.075	2.842ThrThr: 2.842 ± 0.082	4.32ThrVal: 4.32 ± 0.078	0.401ThrTrp: 0.401 ± 0.021	2.18ThrTyr: 2.18 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	4.377ValAla: 4.377 ± 0.074	0.939ValCys: 0.939 ± 0.033	4.611ValAsp: 4.611 ± 0.083	4.674ValGlu: 4.674 ± 0.085	2.871ValPhe: 2.871 ± 0.066	4.129ValGly: 4.129 ± 0.087	0.896ValHis: 0.896 ± 0.032	5.947ValIle: 5.947 ± 0.099	5.309ValLys: 5.309 ± 0.084	5.476ValLeu: 5.476 ± 0.079	1.93ValMet: 1.93 ± 0.054	3.532ValAsn: 3.532 ± 0.068	2.151ValPro: 2.151 ± 0.049	1.333ValGln: 1.333 ± 0.041	2.665ValArg: 2.665 ± 0.063	4.792ValSer: 4.792 ± 0.08	3.887ValThr: 3.887 ± 0.094	4.682ValVal: 4.682 ± 0.086	0.505ValTrp: 0.505 ± 0.027	2.832ValTyr: 2.832 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.453TrpAla: 0.453 ± 0.021	0.142TrpCys: 0.142 ± 0.012	0.521TrpAsp: 0.521 ± 0.029	0.48TrpGlu: 0.48 ± 0.024	0.384TrpPhe: 0.384 ± 0.026	0.505TrpGly: 0.505 ± 0.026	0.157TrpHis: 0.157 ± 0.014	0.647TrpIle: 0.647 ± 0.026	0.571TrpLys: 0.571 ± 0.031	0.673TrpLeu: 0.673 ± 0.031	0.225TrpMet: 0.225 ± 0.017	0.57TrpAsn: 0.57 ± 0.029	0.149TrpPro: 0.149 ± 0.016	0.235TrpGln: 0.235 ± 0.019	0.297TrpArg: 0.297 ± 0.019	0.428TrpSer: 0.428 ± 0.025	0.383TrpThr: 0.383 ± 0.025	0.437TrpVal: 0.437 ± 0.026	0.15TrpTrp: 0.15 ± 0.021	0.409TrpTyr: 0.409 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.507TyrAla: 2.507 ± 0.053	0.601TyrCys: 0.601 ± 0.028	3.513TyrAsp: 3.513 ± 0.097	3.217TyrGlu: 3.217 ± 0.065	2.012TyrPhe: 2.012 ± 0.048	2.902TyrGly: 2.902 ± 0.064	0.76TyrHis: 0.76 ± 0.034	3.793TyrIle: 3.793 ± 0.069	3.62TyrLys: 3.62 ± 0.068	3.499TyrLeu: 3.499 ± 0.068	1.284TyrMet: 1.284 ± 0.045	2.511TyrAsn: 2.511 ± 0.057	1.401TyrPro: 1.401 ± 0.046	1.046TyrGln: 1.046 ± 0.033	1.821TyrArg: 1.821 ± 0.055	3.081TyrSer: 3.081 ± 0.069	2.45TyrThr: 2.45 ± 0.071	2.89TyrVal: 2.89 ± 0.066	0.313TyrTrp: 0.313 ± 0.019	2.225TyrTyr: 2.225 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2481 proteins (845742 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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