Okibacterium fritillariae
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2806 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T5KWD8|A0A1T5KWD8_9MICO Antibiotic biosynthesis monooxygenase OS=Okibacterium fritillariae OX=123320 GN=SAMN06309945_2552 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 EE3 pKa = 3.34 RR4 pKa = 11.84 GARR7 pKa = 11.84 AGARR11 pKa = 11.84 AGSGIGATRR20 pKa = 11.84 RR21 pKa = 11.84 ADD23 pKa = 3.21 ARR25 pKa = 11.84 AISALAVLAAGALALTACSGAGSGDD50 pKa = 3.2 GSGGDD55 pKa = 3.67 TEE57 pKa = 5.35 APASATPAFAIDD69 pKa = 4.07 QDD71 pKa = 4.25 FPDD74 pKa = 4.86 PDD76 pKa = 3.63 VLRR79 pKa = 11.84 VGDD82 pKa = 3.92 TYY84 pKa = 11.5 YY85 pKa = 11.15 AYY87 pKa = 9.46 ATNSPAANLQYY98 pKa = 10.31 ATSTDD103 pKa = 3.6 LEE105 pKa = 4.51 TWQVAGDD112 pKa = 4.0 DD113 pKa = 4.47 AFPDD117 pKa = 3.89 LPAWADD123 pKa = 3.72 EE124 pKa = 4.09 GRR126 pKa = 11.84 TWAPDD131 pKa = 3.34 VTEE134 pKa = 4.29 LADD137 pKa = 4.1 GRR139 pKa = 11.84 FALYY143 pKa = 8.05 FTAQDD148 pKa = 3.57 AEE150 pKa = 4.58 SGQQCIGAATADD162 pKa = 3.8 APTGPFVGASDD173 pKa = 4.06 TPLLCPLDD181 pKa = 3.77 EE182 pKa = 5.38 GGAIDD187 pKa = 5.05 ASSFTDD193 pKa = 3.25 TDD195 pKa = 3.61 GARR198 pKa = 11.84 YY199 pKa = 8.93 LVWKK203 pKa = 10.63 NDD205 pKa = 3.68 GNCCGLDD212 pKa = 2.93 TWLQLSPLGADD223 pKa = 3.52 GVTLAGEE230 pKa = 4.33 PTRR233 pKa = 11.84 LLQQTEE239 pKa = 3.8 AWEE242 pKa = 4.8 GSLIEE247 pKa = 4.29 APVIVKK253 pKa = 10.29 HH254 pKa = 6.73 DD255 pKa = 3.73 DD256 pKa = 3.73 SYY258 pKa = 12.13 VLFYY262 pKa = 10.95 SANDD266 pKa = 3.73 YY267 pKa = 11.26 GGEE270 pKa = 4.11 AYY272 pKa = 8.77 ATGYY276 pKa = 8.43 ATATALAGPYY286 pKa = 8.76 TKK288 pKa = 10.77 ADD290 pKa = 3.6 GPLLTTEE297 pKa = 4.28 ITDD300 pKa = 3.61 DD301 pKa = 4.05 AYY303 pKa = 11.29 VGPGGQDD310 pKa = 3.05 VVAGPDD316 pKa = 3.23 GKK318 pKa = 10.66 DD319 pKa = 2.68 RR320 pKa = 11.84 IFFHH324 pKa = 6.58 SWDD327 pKa = 3.39 PAIVYY332 pKa = 10.19 RR333 pKa = 11.84 GMNVLPLSWNGSTPVVDD350 pKa = 5.3 LPP352 pKa = 4.44
Molecular weight: 36.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.605
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.961
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A1T5IIS8|A0A1T5IIS8_9MICO Excinuclease ABC A subunit OS=Okibacterium fritillariae OX=123320 GN=SAMN06309945_0500 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 10.04 VRR4 pKa = 11.84 SSIKK8 pKa = 10.0 SLKK11 pKa = 8.77 NQPGSQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 VFVINKK30 pKa = 8.85 LNPRR34 pKa = 11.84 WKK36 pKa = 10.37 GRR38 pKa = 11.84 QGG40 pKa = 3.06
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.544
Grimsley 12.837
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.281
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.072
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2806
0
2806
947544
29
2226
337.7
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.086 ± 0.066
0.432 ± 0.01
6.395 ± 0.061
5.429 ± 0.047
3.204 ± 0.034
8.844 ± 0.044
1.867 ± 0.023
4.697 ± 0.038
2.3 ± 0.034
9.809 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.65 ± 0.015
2.329 ± 0.027
5.458 ± 0.037
2.973 ± 0.029
6.687 ± 0.054
6.254 ± 0.035
6.465 ± 0.045
8.713 ± 0.041
1.391 ± 0.02
2.017 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here