Geodermatophilus dictyosporus
Taxonomy:
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4366 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5LXP0|A0A1I5LXP0_9ACTN Carbon-monoxide dehydrogenase large subunit OS=Geodermatophilus dictyosporus OX=1523247 GN=SAMN05660464_1960 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 VRR4 pKa = 11.84 PPAVAALLALALAATGCSTVVAGVASPPPGGLRR37 pKa = 11.84 TDD39 pKa = 3.29 ATDD42 pKa = 3.49 ATDD45 pKa = 4.01 ADD47 pKa = 4.46 LTVHH51 pKa = 6.6 LAEE54 pKa = 5.92 AGDD57 pKa = 3.75 EE58 pKa = 4.17 TDD60 pKa = 4.32 RR61 pKa = 11.84 IARR64 pKa = 11.84 NALADD69 pKa = 3.56 VLTYY73 pKa = 9.93 WDD75 pKa = 3.39 RR76 pKa = 11.84 TYY78 pKa = 11.3 PEE80 pKa = 4.12 VFGGGFGPVDD90 pKa = 3.3 GGFWSIDD97 pKa = 3.4 PDD99 pKa = 3.7 EE100 pKa = 4.99 TDD102 pKa = 4.52 PADD105 pKa = 5.18 LPDD108 pKa = 4.85 GPCFPDD114 pKa = 4.55 DD115 pKa = 4.91 VEE117 pKa = 5.55 DD118 pKa = 4.23 LADD121 pKa = 3.52 NAYY124 pKa = 10.12 YY125 pKa = 10.74 CPDD128 pKa = 3.84 EE129 pKa = 5.02 DD130 pKa = 4.41 LVVYY134 pKa = 10.46 DD135 pKa = 4.31 RR136 pKa = 11.84 AWMAEE141 pKa = 3.84 LAGDD145 pKa = 3.67 YY146 pKa = 11.06 GPFVVAEE153 pKa = 4.34 IMAHH157 pKa = 6.32 EE158 pKa = 4.6 IAHH161 pKa = 6.66 AVQAHH166 pKa = 6.73 AGLDD170 pKa = 3.66 DD171 pKa = 4.22 PSIVAEE177 pKa = 4.24 TQAEE181 pKa = 4.46 CFAGAWTRR189 pKa = 11.84 WVVRR193 pKa = 11.84 GNAAHH198 pKa = 6.55 FSVRR202 pKa = 11.84 PKK204 pKa = 10.82 EE205 pKa = 3.93 LDD207 pKa = 3.63 PYY209 pKa = 11.04 LLGYY213 pKa = 10.47 LYY215 pKa = 10.83 FGDD218 pKa = 4.37 EE219 pKa = 4.51 VGSSPDD225 pKa = 3.38 AEE227 pKa = 4.42 DD228 pKa = 3.14 AHH230 pKa = 7.9 GSVFDD235 pKa = 3.75 QLSAFQEE242 pKa = 5.47 GYY244 pKa = 11.07 ADD246 pKa = 4.26 GPVACAAFDD255 pKa = 3.54 SSRR258 pKa = 11.84 LFTEE262 pKa = 4.41 EE263 pKa = 4.67 EE264 pKa = 3.87 FDD266 pKa = 3.74 GDD268 pKa = 3.64 EE269 pKa = 4.25 AATGGDD275 pKa = 3.57 LSYY278 pKa = 10.6 EE279 pKa = 4.01 AVVAGTGDD287 pKa = 4.23 LLTAFWDD294 pKa = 3.61 QALTRR299 pKa = 11.84 GFPGTDD305 pKa = 3.21 PLGGLLDD312 pKa = 4.46 APDD315 pKa = 4.04 LRR317 pKa = 11.84 AADD320 pKa = 3.86 GTGTVCGGGGEE331 pKa = 5.62 LDD333 pKa = 3.74 LQYY336 pKa = 11.22 CPVDD340 pKa = 3.48 DD341 pKa = 3.87 SVRR344 pKa = 11.84 YY345 pKa = 10.19 DD346 pKa = 3.39 GADD349 pKa = 3.43 LLEE352 pKa = 4.17 PAYY355 pKa = 10.74 AEE357 pKa = 4.19 VGDD360 pKa = 4.14 FAVPTLLGLPYY371 pKa = 11.09 AMAVRR376 pKa = 11.84 EE377 pKa = 3.98 QRR379 pKa = 11.84 GLSVDD384 pKa = 3.53 DD385 pKa = 4.12 AAAVTGSVCATGWLVRR401 pKa = 11.84 EE402 pKa = 4.11 VHH404 pKa = 6.33 RR405 pKa = 11.84 GSVDD409 pKa = 3.42 GLDD412 pKa = 3.9 LALSPGDD419 pKa = 3.39 VDD421 pKa = 3.6 EE422 pKa = 4.97 AAVVLLRR429 pKa = 11.84 YY430 pKa = 8.44 ATEE433 pKa = 4.04 EE434 pKa = 4.09 TVVPVPGLSGFEE446 pKa = 4.02 LVDD449 pKa = 3.36 DD450 pKa = 4.74 FRR452 pKa = 11.84 RR453 pKa = 11.84 GFVDD457 pKa = 4.65 GGAACGFF464 pKa = 4.12
Molecular weight: 48.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.706
IPC_protein 3.757
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.427
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.126
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.872
Patrickios 1.163
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A1I5S9Y9|A0A1I5S9Y9_9ACTN DNA gyrase subunit B OS=Geodermatophilus dictyosporus OX=1523247 GN=gyrB PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 8.58 THH17 pKa = 5.46 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAGRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.08 GRR40 pKa = 11.84 EE41 pKa = 3.61 KK42 pKa = 10.93 LSAA45 pKa = 3.78
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.369
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4366
0
4366
1400948
32
2011
320.9
33.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.832 ± 0.071
0.668 ± 0.01
6.255 ± 0.027
5.479 ± 0.035
2.439 ± 0.021
9.917 ± 0.032
1.989 ± 0.017
2.231 ± 0.028
1.138 ± 0.021
10.811 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.43 ± 0.014
1.264 ± 0.015
6.494 ± 0.034
2.558 ± 0.018
8.515 ± 0.043
4.55 ± 0.025
5.984 ± 0.031
10.283 ± 0.034
1.48 ± 0.018
1.683 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here