Acaryochloris sp. RCC1774
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5409 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W1JSN4|A0A2W1JSN4_9CYAN 3-hydroxyacyl-CoA dehydrogenase OS=Acaryochloris sp. RCC1774 OX=1764569 GN=fadN_1 PE=4 SV=1
MM1 pKa = 7.83 AEE3 pKa = 5.1 DD4 pKa = 3.93 IDD6 pKa = 5.25 HH7 pKa = 6.31 VAQLLEE13 pKa = 4.24 VNACQGCDD21 pKa = 3.36 LSGADD26 pKa = 4.46 LSGLVLINANLQGANLQGANLQNTYY51 pKa = 11.39 LMLSDD56 pKa = 5.01 LADD59 pKa = 4.11 ANLEE63 pKa = 4.2 DD64 pKa = 4.34 TNLTAAYY71 pKa = 9.93 LYY73 pKa = 11.07 GADD76 pKa = 3.96 LQGAALKK83 pKa = 10.34 GANLTQATLRR93 pKa = 11.84 DD94 pKa = 3.87 TQLQGATLCEE104 pKa = 4.09 ADD106 pKa = 4.38 FSGADD111 pKa = 3.39 FRR113 pKa = 11.84 DD114 pKa = 3.6 AEE116 pKa = 4.48 LEE118 pKa = 3.92 VDD120 pKa = 3.56 PDD122 pKa = 3.82 EE123 pKa = 5.29 ACLEE127 pKa = 4.02 RR128 pKa = 11.84 LRR130 pKa = 11.84 AEE132 pKa = 4.4 NAVEE136 pKa = 3.81
Molecular weight: 14.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.617
IPC_protein 3.592
Toseland 3.389
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.846
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|A0A2W1JUF5|A0A2W1JUF5_9CYAN Uncharacterized protein OS=Acaryochloris sp. RCC1774 OX=1764569 GN=C1752_03773 PE=4 SV=1
MM1 pKa = 7.65 GRR3 pKa = 11.84 LLKK6 pKa = 10.79 DD7 pKa = 3.2 KK8 pKa = 8.49 THH10 pKa = 6.71 PTDD13 pKa = 3.12 PMAYY17 pKa = 9.53 QDD19 pKa = 4.08 PQLKK23 pKa = 10.7 AEE25 pKa = 4.48 IGRR28 pKa = 11.84 LHH30 pKa = 6.26 QLTVYY35 pKa = 10.24 GRR37 pKa = 11.84 WLFVFCLWITVGLLSFMGLRR57 pKa = 11.84 SSIDD61 pKa = 2.75 LWLEE65 pKa = 3.8 YY66 pKa = 8.79 FTWAAFRR73 pKa = 11.84 AAFRR77 pKa = 11.84 YY78 pKa = 10.07 NGLATAGLGLCLGTTLAVLLWQSRR102 pKa = 11.84 NILFGLPKK110 pKa = 9.86 QEE112 pKa = 3.85 RR113 pKa = 11.84 TRR115 pKa = 11.84 LRR117 pKa = 11.84 QRR119 pKa = 11.84 VMKK122 pKa = 10.47 IRR124 pKa = 11.84 QQGHH128 pKa = 5.28 NHH130 pKa = 6.6 PLWKK134 pKa = 10.13 LVCQSKK140 pKa = 10.39 SRR142 pKa = 11.84 PQQ144 pKa = 3.02
Molecular weight: 16.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.794
IPC_protein 10.613
Toseland 10.73
ProMoST 10.438
Dawson 10.833
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 11.067
Grimsley 10.891
Solomon 10.95
Lehninger 10.921
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.774
Patrickios 10.804
IPC_peptide 10.965
IPC2_peptide 9.692
IPC2.peptide.svr19 8.439
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5409
0
5409
1670264
29
2972
308.8
34.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.039 ± 0.035
1.032 ± 0.012
5.249 ± 0.034
6.027 ± 0.034
3.783 ± 0.024
6.956 ± 0.042
2.056 ± 0.021
5.755 ± 0.024
3.869 ± 0.032
11.299 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.981 ± 0.018
3.396 ± 0.026
4.987 ± 0.031
5.968 ± 0.04
5.435 ± 0.03
6.622 ± 0.033
5.672 ± 0.023
6.744 ± 0.026
1.438 ± 0.015
2.692 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here