Erysipelotrichaceae bacterium 5_2_54FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; unclassified Erysipelotrichaceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2871 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5F9C0|U5F9C0_9FIRM ABC transporter domain-containing protein OS=Erysipelotrichaceae bacterium 5_2_54FAA OX=552396 GN=HMPREF0863_01010 PE=4 SV=1
MM1 pKa = 6.74VQVKK5 pKa = 9.33VFDD8 pKa = 4.4EE9 pKa = 4.1EE10 pKa = 5.42HH11 pKa = 6.89EE12 pKa = 5.02DD13 pKa = 4.83DD14 pKa = 5.47LSDD17 pKa = 4.08MINAFLEE24 pKa = 4.54DD25 pKa = 3.97NPDD28 pKa = 3.27IDD30 pKa = 4.07VVDD33 pKa = 3.87IKK35 pKa = 11.09FSTSCSIQEE44 pKa = 4.15EE45 pKa = 4.16EE46 pKa = 4.82QIYY49 pKa = 10.36CFSALLIYY57 pKa = 9.29RR58 pKa = 11.84TT59 pKa = 3.89

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4T008|U4T008_9FIRM Uncharacterized protein OS=Erysipelotrichaceae bacterium 5_2_54FAA OX=552396 GN=HMPREF0863_04170 PE=4 SV=1
MM1 pKa = 6.79QAYY4 pKa = 9.47IARR7 pKa = 11.84TPNVSKK13 pKa = 11.1KK14 pKa = 9.17EE15 pKa = 3.77LEE17 pKa = 4.13RR18 pKa = 11.84RR19 pKa = 11.84QKK21 pKa = 10.16EE22 pKa = 4.06CRR24 pKa = 11.84GDD26 pKa = 3.16QRR28 pKa = 11.84LRR30 pKa = 11.84IEE32 pKa = 4.01ARR34 pKa = 11.84FGRR37 pKa = 11.84NVRR40 pKa = 11.84SLRR43 pKa = 11.84KK44 pKa = 9.18QRR46 pKa = 11.84HH47 pKa = 4.2LTQEE51 pKa = 3.76EE52 pKa = 4.41LAALCHH58 pKa = 5.11VHH60 pKa = 5.54QHH62 pKa = 5.86YY63 pKa = 10.7ISDD66 pKa = 3.56IEE68 pKa = 4.16RR69 pKa = 11.84GVRR72 pKa = 11.84NVSLRR77 pKa = 11.84VVEE80 pKa = 5.07TIARR84 pKa = 11.84ALGVHH89 pKa = 6.27EE90 pKa = 5.47KK91 pKa = 11.07DD92 pKa = 4.09LLL94 pKa = 4.31

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2871

0

2871

916794

29

5801

319.3

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.222 ± 0.052

1.489 ± 0.024

5.757 ± 0.039

7.027 ± 0.054

4.174 ± 0.032

6.056 ± 0.05

2.372 ± 0.03

7.768 ± 0.047

7.597 ± 0.053

9.367 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.226 ± 0.039

4.403 ± 0.044

3.016 ± 0.023

4.003 ± 0.036

3.857 ± 0.042

5.568 ± 0.036

5.47 ± 0.06

6.37 ± 0.034

0.844 ± 0.014

4.414 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski