Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Dickeya; Dickeya dadantii

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0SLT1|E0SLT1_DICD3 ABC transporter periplasmic binding protein OS=Dickeya dadantii (strain 3937) OX=198628 GN=Dda3937_02271 PE=4 SV=1
MM1 pKa = 7.4SNVPAEE7 pKa = 4.23LKK9 pKa = 9.24YY10 pKa = 9.3TSSHH14 pKa = 4.73EE15 pKa = 3.91WVMAEE20 pKa = 3.9GNGVYY25 pKa = 10.24SVGITEE31 pKa = 4.64HH32 pKa = 6.12AQEE35 pKa = 4.67LLGDD39 pKa = 3.92MVFIDD44 pKa = 4.33LPEE47 pKa = 4.28VGSTVNAGDD56 pKa = 3.79DD57 pKa = 3.75CAVAEE62 pKa = 4.4SVKK65 pKa = 10.54AASDD69 pKa = 3.51IYY71 pKa = 11.29APISGEE77 pKa = 3.88VVEE80 pKa = 4.83VNEE83 pKa = 4.25EE84 pKa = 4.15LEE86 pKa = 4.38GAPEE90 pKa = 4.35LVNSAPYY97 pKa = 10.53AEE99 pKa = 4.47GWLFRR104 pKa = 11.84IKK106 pKa = 10.78ASDD109 pKa = 3.57EE110 pKa = 4.4DD111 pKa = 4.19EE112 pKa = 4.95LNEE115 pKa = 4.22LLDD118 pKa = 3.94AAGYY122 pKa = 8.88QALLEE127 pKa = 4.65EE128 pKa = 4.65EE129 pKa = 5.17DD130 pKa = 3.82SDD132 pKa = 4.78EE133 pKa = 4.53EE134 pKa = 4.21EE135 pKa = 4.1

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P45416|KDGK_DICD3 2-dehydro-3-deoxygluconokinase OS=Dickeya dadantii (strain 3937) OX=198628 GN=kdgK PE=2 SV=3
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4532

0

4532

1412289

32

4215

311.6

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.029 ± 0.051

1.088 ± 0.014

5.277 ± 0.03

5.197 ± 0.043

3.732 ± 0.025

7.38 ± 0.046

2.346 ± 0.017

5.45 ± 0.034

3.647 ± 0.039

11.143 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.019

3.578 ± 0.03

4.681 ± 0.027

4.845 ± 0.035

6.24 ± 0.042

6.145 ± 0.033

5.366 ± 0.045

7.01 ± 0.028

1.423 ± 0.016

2.814 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski