Legionella adelaidensis
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2194 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0R498|A0A0W0R498_9GAMM Uncharacterized protein OS=Legionella adelaidensis OX=45056 GN=Lade_0521 PE=4 SV=1
MM1 pKa = 7.6 IRR3 pKa = 11.84 ILTLLFITTLSFTANADD20 pKa = 3.78 DD21 pKa = 4.98 ALSSTDD27 pKa = 3.54 TQDD30 pKa = 3.53 QQNDD34 pKa = 3.26 QQTCYY39 pKa = 7.61 QQRR42 pKa = 11.84 ISDD45 pKa = 4.35 CMNKK49 pKa = 9.79 CASSDD54 pKa = 3.5 ASDD57 pKa = 3.95 DD58 pKa = 4.08 CEE60 pKa = 4.45 SLCEE64 pKa = 3.94 EE65 pKa = 4.54 NSRR68 pKa = 11.84 NEE70 pKa = 4.12 CKK72 pKa = 10.44 DD73 pKa = 3.01 AGEE76 pKa = 4.03
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.528
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A0W0R554|A0A0W0R554_9GAMM Transmembrane permease OS=Legionella adelaidensis OX=45056 GN=ydiK PE=3 SV=1
MM1 pKa = 6.58 NTRR4 pKa = 11.84 MQVAMQQEE12 pKa = 5.24 VYY14 pKa = 10.91 AQIKK18 pKa = 9.87 DD19 pKa = 3.21 GRR21 pKa = 11.84 LRR23 pKa = 11.84 PRR25 pKa = 11.84 KK26 pKa = 9.32 CWLCKK31 pKa = 10.13 SGEE34 pKa = 4.22 EE35 pKa = 3.88 IAAILPEE42 pKa = 3.86 RR43 pKa = 11.84 AAIKK47 pKa = 10.08 RR48 pKa = 11.84 SYY50 pKa = 8.74 PAIMRR55 pKa = 11.84 AALNVLANARR65 pKa = 11.84 DD66 pKa = 3.89 RR67 pKa = 11.84 RR68 pKa = 11.84 FSPYY72 pKa = 10.64 LVGG75 pKa = 4.83
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.648
IPC_protein 10.438
Toseland 10.482
ProMoST 10.204
Dawson 10.628
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.833
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.599
IPC_peptide 10.716
IPC2_peptide 9.502
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2194
0
2194
718026
51
3010
327.3
36.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.755 ± 0.057
1.13 ± 0.021
4.522 ± 0.035
6.41 ± 0.055
4.658 ± 0.039
6.113 ± 0.06
2.354 ± 0.026
7.535 ± 0.04
6.437 ± 0.043
10.937 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.024
4.789 ± 0.034
4.272 ± 0.034
4.379 ± 0.038
4.319 ± 0.035
6.343 ± 0.04
5.254 ± 0.033
6.074 ± 0.044
1.117 ± 0.019
3.334 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here