Cryobacterium tepidiphilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8KU41|A0A3M8KU41_9MICO DUF58 domain-containing protein OS=Cryobacterium tepidiphilum OX=2486026 GN=EEJ31_12770 PE=4 SV=1
MM1 pKa = 7.43NNHH4 pKa = 6.76GGNGFVTIYY13 pKa = 10.13YY14 pKa = 9.18PSRR17 pKa = 11.84LTSISPATGLTGDD30 pKa = 4.48ALTIDD35 pKa = 4.02GSGLTGATVTIAGVAATVTSSSDD58 pKa = 3.16TQLEE62 pKa = 4.39VTVPSQSPLPAGAQRR77 pKa = 11.84VDD79 pKa = 3.21VTTAGGVTLPAVGAFTYY96 pKa = 10.57EE97 pKa = 3.94PAPTVAGVNPASIPQGSAPTVTITGTHH124 pKa = 4.97FTGATDD130 pKa = 3.76VYY132 pKa = 10.72FGATPATAFTVTSDD146 pKa = 3.31TTITATVPSNLPSGIVDD163 pKa = 3.67TTVVTPNATSVTGAADD179 pKa = 3.63EE180 pKa = 4.53LTVVPYY186 pKa = 8.54PTMTLSPGALPSAQVGVAFSSQLSAGGGTSPYY218 pKa = 9.71TYY220 pKa = 10.34AITSGSLPDD229 pKa = 5.91GITLDD234 pKa = 3.82AASGALTGTPTADD247 pKa = 2.94GSFTFTIEE255 pKa = 4.17ATDD258 pKa = 3.44QYY260 pKa = 11.36GNTISANYY268 pKa = 9.66SVDD271 pKa = 2.93VMAAVPTITDD281 pKa = 4.18LSRR284 pKa = 11.84VSGPTNGGTDD294 pKa = 3.59LTITGTDD301 pKa = 3.39LTGATAVMIGSTPVKK316 pKa = 9.75SFTVVSPTTITAITDD331 pKa = 3.22ASSAGDD337 pKa = 3.41FTVTVTTPGGTSATSPDD354 pKa = 3.21VHH356 pKa = 7.29FSYY359 pKa = 10.89VAAPTVTAVSPTSGAGIGGASVTITGTGFTGALAVDD395 pKa = 4.89FGGTPARR402 pKa = 11.84SYY404 pKa = 9.9TVNSDD409 pKa = 3.29TSITAVAPTSSTGTVHH425 pKa = 5.56VTVTTAGGVSTTSAADD441 pKa = 3.13QFTFEE446 pKa = 4.56APATAVPPTVPTHH459 pKa = 6.74DD460 pKa = 3.65QLAHH464 pKa = 6.06TGADD468 pKa = 2.49IGTPLSLAGGLLLAGLITAALALVARR494 pKa = 11.84RR495 pKa = 11.84RR496 pKa = 11.84RR497 pKa = 11.84DD498 pKa = 3.1ARR500 pKa = 3.64

Molecular weight: 48.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8L9F9|A0A3M8L9F9_9MICO Winged helix family transcriptional regulator OS=Cryobacterium tepidiphilum OX=2486026 GN=EEJ31_09140 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2649

0

2649

858339

32

1953

324.0

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.297 ± 0.066

0.525 ± 0.01

6.1 ± 0.038

5.416 ± 0.049

3.139 ± 0.027

8.965 ± 0.047

2.039 ± 0.022

4.545 ± 0.033

2.283 ± 0.036

10.26 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.02

2.191 ± 0.025

5.365 ± 0.031

2.918 ± 0.022

6.971 ± 0.053

5.778 ± 0.04

6.042 ± 0.046

8.911 ± 0.044

1.383 ± 0.02

2.028 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski