Amino acid dipepetide frequency for Cryobacterium tepidiphilum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.871AlaAla: 19.871 ± 0.23
0.698AlaCys: 0.698 ± 0.033
8.183AlaAsp: 8.183 ± 0.115
7.669AlaGlu: 7.669 ± 0.114
3.807AlaPhe: 3.807 ± 0.073
12.62AlaGly: 12.62 ± 0.139
2.452AlaHis: 2.452 ± 0.059
5.653AlaIle: 5.653 ± 0.08
3.115AlaLys: 3.115 ± 0.078
14.09AlaLeu: 14.09 ± 0.185
2.776AlaMet: 2.776 ± 0.046
2.532AlaAsn: 2.532 ± 0.05
6.342AlaPro: 6.342 ± 0.119
3.687AlaGln: 3.687 ± 0.073
8.767AlaArg: 8.767 ± 0.125
7.749AlaSer: 7.749 ± 0.107
7.485AlaThr: 7.485 ± 0.118
11.366AlaVal: 11.366 ± 0.139
1.828AlaTrp: 1.828 ± 0.052
2.279AlaTyr: 2.279 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.634CysAla: 0.634 ± 0.03
0.062CysCys: 0.062 ± 0.01
0.326CysAsp: 0.326 ± 0.018
0.267CysGlu: 0.267 ± 0.02
0.183CysPhe: 0.183 ± 0.016
0.597CysGly: 0.597 ± 0.027
0.129CysHis: 0.129 ± 0.013
0.217CysIle: 0.217 ± 0.017
0.065CysLys: 0.065 ± 0.009
0.468CysLeu: 0.468 ± 0.023
0.072CysMet: 0.072 ± 0.009
0.106CysAsn: 0.106 ± 0.011
0.306CysPro: 0.306 ± 0.02
0.125CysGln: 0.125 ± 0.012
0.337CysArg: 0.337 ± 0.021
0.365CysSer: 0.365 ± 0.02
0.361CysThr: 0.361 ± 0.021
0.439CysVal: 0.439 ± 0.025
0.084CysTrp: 0.084 ± 0.009
0.105CysTyr: 0.105 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.769AspAla: 8.769 ± 0.107
0.259AspCys: 0.259 ± 0.018
4.125AspAsp: 4.125 ± 0.087
4.141AspGlu: 4.141 ± 0.09
1.892AspPhe: 1.892 ± 0.049
5.618AspGly: 5.618 ± 0.082
1.226AspHis: 1.226 ± 0.041
2.569AspIle: 2.569 ± 0.055
1.176AspLys: 1.176 ± 0.045
6.206AspLeu: 6.206 ± 0.08
0.83AspMet: 0.83 ± 0.034
1.099AspAsn: 1.099 ± 0.039
3.807AspPro: 3.807 ± 0.074
1.702AspGln: 1.702 ± 0.046
4.118AspArg: 4.118 ± 0.076
2.734AspSer: 2.734 ± 0.056
3.038AspThr: 3.038 ± 0.061
5.449AspVal: 5.449 ± 0.093
0.878AspTrp: 0.878 ± 0.032
1.368AspTyr: 1.368 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.803GluAla: 6.803 ± 0.103
0.235GluCys: 0.235 ± 0.018
2.459GluAsp: 2.459 ± 0.055
2.868GluGlu: 2.868 ± 0.077
1.72GluPhe: 1.72 ± 0.05
3.579GluGly: 3.579 ± 0.072
1.443GluHis: 1.443 ± 0.041
2.659GluIle: 2.659 ± 0.058
1.509GluLys: 1.509 ± 0.051
6.335GluLeu: 6.335 ± 0.113
0.936GluMet: 0.936 ± 0.035
1.397GluAsn: 1.397 ± 0.041
2.993GluPro: 2.993 ± 0.057
2.235GluGln: 2.235 ± 0.054
4.922GluArg: 4.922 ± 0.091
2.908GluSer: 2.908 ± 0.061
3.04GluThr: 3.04 ± 0.068
4.251GluVal: 4.251 ± 0.074
0.765GluTrp: 0.765 ± 0.032
1.107GluTyr: 1.107 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
4.008PheAla: 4.008 ± 0.074
0.183PheCys: 0.183 ± 0.015
2.37PheAsp: 2.37 ± 0.056
1.735PheGlu: 1.735 ± 0.052
1.068PhePhe: 1.068 ± 0.039
3.403PheGly: 3.403 ± 0.06
0.599PheHis: 0.599 ± 0.026
1.327PheIle: 1.327 ± 0.042
0.576PheLys: 0.576 ± 0.028
2.963PheLeu: 2.963 ± 0.068
0.51PheMet: 0.51 ± 0.025
0.789PheAsn: 0.789 ± 0.033
1.424PhePro: 1.424 ± 0.046
0.82PheGln: 0.82 ± 0.032
1.735PheArg: 1.735 ± 0.049
1.836PheSer: 1.836 ± 0.045
2.167PheThr: 2.167 ± 0.062
2.754PheVal: 2.754 ± 0.061
0.431PheTrp: 0.431 ± 0.022
0.694PheTyr: 0.694 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
10.489GlyAla: 10.489 ± 0.139
0.662GlyCys: 0.662 ± 0.03
5.055GlyAsp: 5.055 ± 0.085
4.78GlyGlu: 4.78 ± 0.073
3.294GlyPhe: 3.294 ± 0.068
7.509GlyGly: 7.509 ± 0.109
1.961GlyHis: 1.961 ± 0.045
4.877GlyIle: 4.877 ± 0.07
2.364GlyLys: 2.364 ± 0.058
9.123GlyLeu: 9.123 ± 0.12
1.919GlyMet: 1.919 ± 0.051
2.013GlyAsn: 2.013 ± 0.056
3.697GlyPro: 3.697 ± 0.07
2.779GlyGln: 2.779 ± 0.061
6.233GlyArg: 6.233 ± 0.083
5.755GlySer: 5.755 ± 0.086
5.762GlyThr: 5.762 ± 0.103
7.621GlyVal: 7.621 ± 0.107
1.526GlyTrp: 1.526 ± 0.047
2.233GlyTyr: 2.233 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
2.378HisAla: 2.378 ± 0.056
0.121HisCys: 0.121 ± 0.011
1.397HisAsp: 1.397 ± 0.039
1.138HisGlu: 1.138 ± 0.033
0.546HisPhe: 0.546 ± 0.028
2.046HisGly: 2.046 ± 0.051
0.552HisHis: 0.552 ± 0.03
0.797HisIle: 0.797 ± 0.03
0.329HisLys: 0.329 ± 0.021
2.089HisLeu: 2.089 ± 0.047
0.319HisMet: 0.319 ± 0.021
0.43HisAsn: 0.43 ± 0.021
1.553HisPro: 1.553 ± 0.043
0.55HisGln: 0.55 ± 0.023
1.525HisArg: 1.525 ± 0.049
1.102HisSer: 1.102 ± 0.039
1.049HisThr: 1.049 ± 0.035
1.702HisVal: 1.702 ± 0.045
0.287HisTrp: 0.287 ± 0.019
0.48HisTyr: 0.48 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.473IleAla: 6.473 ± 0.091
0.266IleCys: 0.266 ± 0.017
3.487IleAsp: 3.487 ± 0.071
2.788IleGlu: 2.788 ± 0.065
1.244IlePhe: 1.244 ± 0.048
4.534IleGly: 4.534 ± 0.081
0.755IleHis: 0.755 ± 0.029
1.95IleIle: 1.95 ± 0.06
0.94IleLys: 0.94 ± 0.037
3.874IleLeu: 3.874 ± 0.075
0.671IleMet: 0.671 ± 0.031
1.057IleAsn: 1.057 ± 0.037
2.482IlePro: 2.482 ± 0.058
0.973IleGln: 0.973 ± 0.038
2.921IleArg: 2.921 ± 0.055
2.406IleSer: 2.406 ± 0.051
2.917IleThr: 2.917 ± 0.061
4.442IleVal: 4.442 ± 0.08
0.493IleTrp: 0.493 ± 0.024
0.775IleTyr: 0.775 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
2.963LysAla: 2.963 ± 0.075
0.068LysCys: 0.068 ± 0.009
1.278LysAsp: 1.278 ± 0.043
1.085LysGlu: 1.085 ± 0.046
0.522LysPhe: 0.522 ± 0.026
1.729LysGly: 1.729 ± 0.05
0.489LysHis: 0.489 ± 0.027
0.99LysIle: 0.99 ± 0.04
0.941LysLys: 0.941 ± 0.047
2.237LysLeu: 2.237 ± 0.056
0.43LysMet: 0.43 ± 0.023
0.659LysAsn: 0.659 ± 0.029
1.431LysPro: 1.431 ± 0.043
0.864LysGln: 0.864 ± 0.033
1.749LysArg: 1.749 ± 0.048
1.337LysSer: 1.337 ± 0.044
1.433LysThr: 1.433 ± 0.044
1.875LysVal: 1.875 ± 0.059
0.242LysTrp: 0.242 ± 0.016
0.508LysTyr: 0.508 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
15.056LeuAla: 15.056 ± 0.16
0.527LeuCys: 0.527 ± 0.025
6.316LeuAsp: 6.316 ± 0.091
5.105LeuGlu: 5.105 ± 0.081
2.985LeuPhe: 2.985 ± 0.069
9.546LeuGly: 9.546 ± 0.131
1.94LeuHis: 1.94 ± 0.049
4.513LeuIle: 4.513 ± 0.09
2.055LeuLys: 2.055 ± 0.055
10.583LeuLeu: 10.583 ± 0.173
1.71LeuMet: 1.71 ± 0.048
1.999LeuAsn: 1.999 ± 0.055
5.741LeuPro: 5.741 ± 0.09
2.67LeuGln: 2.67 ± 0.054
7.039LeuArg: 7.039 ± 0.103
5.976LeuSer: 5.976 ± 0.094
6.305LeuThr: 6.305 ± 0.088
9.592LeuVal: 9.592 ± 0.145
1.216LeuTrp: 1.216 ± 0.041
1.728LeuTyr: 1.728 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
2.247MetAla: 2.247 ± 0.055
0.104MetCys: 0.104 ± 0.013
0.903MetAsp: 0.903 ± 0.036
0.719MetGlu: 0.719 ± 0.028
0.539MetPhe: 0.539 ± 0.025
1.342MetGly: 1.342 ± 0.04
0.351MetHis: 0.351 ± 0.02
0.896MetIle: 0.896 ± 0.031
0.527MetLys: 0.527 ± 0.03
1.944MetLeu: 1.944 ± 0.051
0.343MetMet: 0.343 ± 0.021
0.49MetAsn: 0.49 ± 0.022
1.114MetPro: 1.114 ± 0.04
0.573MetGln: 0.573 ± 0.027
1.357MetArg: 1.357 ± 0.043
1.454MetSer: 1.454 ± 0.035
1.621MetThr: 1.621 ± 0.048
1.448MetVal: 1.448 ± 0.045
0.182MetTrp: 0.182 ± 0.015
0.271MetTyr: 0.271 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.657AsnAla: 2.657 ± 0.067
0.115AsnCys: 0.115 ± 0.011
1.332AsnAsp: 1.332 ± 0.045
1.109AsnGlu: 1.109 ± 0.034
0.691AsnPhe: 0.691 ± 0.027
2.143AsnGly: 2.143 ± 0.054
0.4AsnHis: 0.4 ± 0.024
0.981AsnIle: 0.981 ± 0.038
0.508AsnLys: 0.508 ± 0.028
2.118AsnLeu: 2.118 ± 0.052
0.348AsnMet: 0.348 ± 0.021
0.543AsnAsn: 0.543 ± 0.027
1.621AsnPro: 1.621 ± 0.042
0.718AsnGln: 0.718 ± 0.032
1.519AsnArg: 1.519 ± 0.044
1.122AsnSer: 1.122 ± 0.041
1.276AsnThr: 1.276 ± 0.041
1.833AsnVal: 1.833 ± 0.053
0.312AsnTrp: 0.312 ± 0.018
0.563AsnTyr: 0.563 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
7.683ProAla: 7.683 ± 0.12
0.202ProCys: 0.202 ± 0.015
3.744ProAsp: 3.744 ± 0.078
3.644ProGlu: 3.644 ± 0.078
1.679ProPhe: 1.679 ± 0.044
5.382ProGly: 5.382 ± 0.09
1.13ProHis: 1.13 ± 0.03
2.023ProIle: 2.023 ± 0.049
1.149ProLys: 1.149 ± 0.044
4.882ProLeu: 4.882 ± 0.078
0.89ProMet: 0.89 ± 0.033
1.13ProAsn: 1.13 ± 0.035
2.233ProPro: 2.233 ± 0.067
1.435ProGln: 1.435 ± 0.043
3.108ProArg: 3.108 ± 0.068
3.128ProSer: 3.128 ± 0.064
3.223ProThr: 3.223 ± 0.064
5.124ProVal: 5.124 ± 0.074
0.782ProTrp: 0.782 ± 0.031
1.082ProTyr: 1.082 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.948GlnAla: 3.948 ± 0.074
0.105GlnCys: 0.105 ± 0.011
1.289GlnAsp: 1.289 ± 0.051
1.407GlnGlu: 1.407 ± 0.046
0.868GlnPhe: 0.868 ± 0.033
2.188GlnGly: 2.188 ± 0.049
0.605GlnHis: 0.605 ± 0.028
1.319GlnIle: 1.319 ± 0.041
0.845GlnLys: 0.845 ± 0.036
3.36GlnLeu: 3.36 ± 0.068
0.52GlnMet: 0.52 ± 0.03
0.72GlnAsn: 0.72 ± 0.031
1.663GlnPro: 1.663 ± 0.046
1.179GlnGln: 1.179 ± 0.041
2.356GlnArg: 2.356 ± 0.056
1.538GlnSer: 1.538 ± 0.042
1.447GlnThr: 1.447 ± 0.042
2.803GlnVal: 2.803 ± 0.052
0.416GlnTrp: 0.416 ± 0.023
0.602GlnTyr: 0.602 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
8.197ArgAla: 8.197 ± 0.119
0.315ArgCys: 0.315 ± 0.019
3.934ArgAsp: 3.934 ± 0.073
4.061ArgGlu: 4.061 ± 0.079
2.482ArgPhe: 2.482 ± 0.053
5.222ArgGly: 5.222 ± 0.086
1.625ArgHis: 1.625 ± 0.045
3.339ArgIle: 3.339 ± 0.067
1.553ArgLys: 1.553 ± 0.05
7.368ArgLeu: 7.368 ± 0.096
1.793ArgMet: 1.793 ± 0.05
1.552ArgAsn: 1.552 ± 0.042
3.465ArgPro: 3.465 ± 0.077
2.264ArgGln: 2.264 ± 0.057
6.047ArgArg: 6.047 ± 0.105
4.127ArgSer: 4.127 ± 0.076
3.952ArgThr: 3.952 ± 0.079
5.745ArgVal: 5.745 ± 0.099
1.045ArgTrp: 1.045 ± 0.037
1.63ArgTyr: 1.63 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
7.365SerAla: 7.365 ± 0.111
0.298SerCys: 0.298 ± 0.02
3.165SerAsp: 3.165 ± 0.065
2.709SerGlu: 2.709 ± 0.057
1.95SerPhe: 1.95 ± 0.053
6.125SerGly: 6.125 ± 0.094
1.128SerHis: 1.128 ± 0.037
2.664SerIle: 2.664 ± 0.055
1.235SerLys: 1.235 ± 0.04
5.522SerLeu: 5.522 ± 0.094
1.24SerMet: 1.24 ± 0.041
1.216SerAsn: 1.216 ± 0.04
3.198SerPro: 3.198 ± 0.058
1.553SerGln: 1.553 ± 0.042
3.812SerArg: 3.812 ± 0.052
3.577SerSer: 3.577 ± 0.082
3.626SerThr: 3.626 ± 0.071
5.275SerVal: 5.275 ± 0.085
0.862SerTrp: 0.862 ± 0.033
1.259SerTyr: 1.259 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
7.844ThrAla: 7.844 ± 0.13
0.298ThrCys: 0.298 ± 0.022
3.89ThrAsp: 3.89 ± 0.087
2.859ThrGlu: 2.859 ± 0.059
1.857ThrPhe: 1.857 ± 0.048
6.034ThrGly: 6.034 ± 0.079
1.183ThrHis: 1.183 ± 0.038
2.762ThrIle: 2.762 ± 0.056
1.339ThrLys: 1.339 ± 0.046
6.04ThrLeu: 6.04 ± 0.083
0.995ThrMet: 0.995 ± 0.037
1.266ThrAsn: 1.266 ± 0.04
3.898ThrPro: 3.898 ± 0.078
1.42ThrGln: 1.42 ± 0.041
3.726ThrArg: 3.726 ± 0.066
3.444ThrSer: 3.444 ± 0.073
3.75ThrThr: 3.75 ± 0.075
5.85ThrVal: 5.85 ± 0.099
0.803ThrTrp: 0.803 ± 0.035
1.164ThrTyr: 1.164 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
11.682ValAla: 11.682 ± 0.138
0.5ValCys: 0.5 ± 0.025
5.669ValAsp: 5.669 ± 0.099
4.418ValGlu: 4.418 ± 0.074
2.863ValPhe: 2.863 ± 0.057
7.158ValGly: 7.158 ± 0.111
1.793ValHis: 1.793 ± 0.05
4.614ValIle: 4.614 ± 0.083
1.859ValLys: 1.859 ± 0.049
9.397ValLeu: 9.397 ± 0.144
1.569ValMet: 1.569 ± 0.048
2.009ValAsn: 2.009 ± 0.053
4.85ValPro: 4.85 ± 0.072
2.39ValGln: 2.39 ± 0.052
5.662ValArg: 5.662 ± 0.089
5.172ValSer: 5.172 ± 0.096
6.009ValThr: 6.009 ± 0.102
8.846ValVal: 8.846 ± 0.124
1.033ValTrp: 1.033 ± 0.037
1.614ValTyr: 1.614 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.53TrpAla: 1.53 ± 0.042
0.103TrpCys: 0.103 ± 0.009
0.722TrpAsp: 0.722 ± 0.031
0.607TrpGlu: 0.607 ± 0.023
0.504TrpPhe: 0.504 ± 0.026
0.939TrpGly: 0.939 ± 0.033
0.326TrpHis: 0.326 ± 0.019
0.597TrpIle: 0.597 ± 0.022
0.341TrpLys: 0.341 ± 0.02
1.736TrpLeu: 1.736 ± 0.047
0.288TrpMet: 0.288 ± 0.017
0.421TrpAsn: 0.421 ± 0.026
0.726TrpPro: 0.726 ± 0.028
0.553TrpGln: 0.553 ± 0.027
1.115TrpArg: 1.115 ± 0.036
0.833TrpSer: 0.833 ± 0.037
0.788TrpThr: 0.788 ± 0.033
1.072TrpVal: 1.072 ± 0.036
0.306TrpTrp: 0.306 ± 0.019
0.327TrpTyr: 0.327 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.374TyrAla: 2.374 ± 0.054
0.129TyrCys: 0.129 ± 0.012
1.358TyrAsp: 1.358 ± 0.045
1.054TyrGlu: 1.054 ± 0.037
0.697TyrPhe: 0.697 ± 0.032
1.939TyrGly: 1.939 ± 0.05
0.303TyrHis: 0.303 ± 0.017
0.701TyrIle: 0.701 ± 0.03
0.405TyrLys: 0.405 ± 0.023
2.267TyrLeu: 2.267 ± 0.053
0.266TyrMet: 0.266 ± 0.016
0.48TyrAsn: 0.48 ± 0.023
1.11TyrPro: 1.11 ± 0.035
0.686TyrGln: 0.686 ± 0.032
1.666TyrArg: 1.666 ± 0.045
1.219TyrSer: 1.219 ± 0.04
1.173TyrThr: 1.173 ± 0.041
1.619TyrVal: 1.619 ± 0.047
0.341TyrTrp: 0.341 ± 0.022
0.495TyrTyr: 0.495 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2649 proteins (858339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski