Mycobacterium virus Courthouse

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8I5I9|G8I5I9_9CAUD Uncharacterized protein OS=Mycobacterium virus Courthouse OX=1089119 GN=132 PE=4 SV=1
MM1 pKa = 7.85SYY3 pKa = 10.0PHH5 pKa = 6.73HH6 pKa = 7.23AEE8 pKa = 4.18DD9 pKa = 5.04GEE11 pKa = 4.3LGAIYY16 pKa = 9.08VTLTPNPVAKK26 pKa = 9.43TVSLDD31 pKa = 3.51DD32 pKa = 3.66GAINVDD38 pKa = 3.4YY39 pKa = 10.97DD40 pKa = 3.86ADD42 pKa = 3.95GNAVGVEE49 pKa = 3.85ILLL52 pKa = 4.13

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8I5G0|G8I5G0_9CAUD Uncharacterized protein OS=Mycobacterium virus Courthouse OX=1089119 GN=103 PE=4 SV=1
MM1 pKa = 7.12RR2 pKa = 11.84TCTIPGCEE10 pKa = 4.32KK11 pKa = 9.83KK12 pKa = 9.56HH13 pKa = 5.72HH14 pKa = 6.39ARR16 pKa = 11.84GFCVVHH22 pKa = 4.36YY23 pKa = 8.87QRR25 pKa = 11.84WRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SDD31 pKa = 3.36EE32 pKa = 3.4PRR34 pKa = 11.84GNYY37 pKa = 8.7RR38 pKa = 11.84RR39 pKa = 11.84NHH41 pKa = 6.12RR42 pKa = 11.84PDD44 pKa = 5.51CKK46 pKa = 10.92LEE48 pKa = 3.7WCNGRR53 pKa = 11.84FYY55 pKa = 11.42AKK57 pKa = 10.02DD58 pKa = 3.06LCRR61 pKa = 11.84FHH63 pKa = 6.88HH64 pKa = 6.03QRR66 pKa = 11.84LLRR69 pKa = 11.84GKK71 pKa = 10.72AMDD74 pKa = 4.31DD75 pKa = 3.16PRR77 pKa = 11.84GNVGIFDD84 pKa = 4.07WAACGTPAQYY94 pKa = 10.22RR95 pKa = 11.84KK96 pKa = 9.65HH97 pKa = 5.68FRR99 pKa = 11.84HH100 pKa = 7.05GIPLCDD106 pKa = 3.63ACRR109 pKa = 11.84KK110 pKa = 10.13AEE112 pKa = 3.99SRR114 pKa = 11.84RR115 pKa = 11.84QKK117 pKa = 10.83DD118 pKa = 3.09RR119 pKa = 11.84RR120 pKa = 11.84EE121 pKa = 3.73KK122 pKa = 10.82AKK124 pKa = 10.59RR125 pKa = 3.35

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

34605

22

1617

143.6

15.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.764 ± 0.259

1.578 ± 0.138

6.534 ± 0.16

6.93 ± 0.255

3.161 ± 0.093

8.822 ± 0.4

2.332 ± 0.111

4.878 ± 0.133

3.997 ± 0.166

7.857 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.107

3.26 ± 0.106

5.306 ± 0.143

3.026 ± 0.153

6.802 ± 0.243

5.453 ± 0.173

5.554 ± 0.19

6.826 ± 0.207

2.274 ± 0.118

3.17 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski