Amino acid dipepetide frequency for Mycobacterium virus Courthouse

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.126AlaAla: 11.126 ± 0.829
1.676AlaCys: 1.676 ± 0.285
6.791AlaAsp: 6.791 ± 0.456
7.803AlaGlu: 7.803 ± 0.614
3.179AlaPhe: 3.179 ± 0.336
7.311AlaGly: 7.311 ± 0.708
1.994AlaHis: 1.994 ± 0.231
5.375AlaIle: 5.375 ± 0.394
4.104AlaLys: 4.104 ± 0.358
8.583AlaLeu: 8.583 ± 0.549
3.092AlaMet: 3.092 ± 0.361
3.294AlaAsn: 3.294 ± 0.355
4.132AlaPro: 4.132 ± 0.376
2.688AlaGln: 2.688 ± 0.291
6.762AlaArg: 6.762 ± 0.471
4.306AlaSer: 4.306 ± 0.327
4.884AlaThr: 4.884 ± 0.397
5.809AlaVal: 5.809 ± 0.436
1.821AlaTrp: 1.821 ± 0.239
2.919AlaTyr: 2.919 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
1.3CysAla: 1.3 ± 0.202
0.116CysCys: 0.116 ± 0.059
1.445CysAsp: 1.445 ± 0.241
1.098CysGlu: 1.098 ± 0.236
0.433CysPhe: 0.433 ± 0.128
2.196CysGly: 2.196 ± 0.312
0.578CysHis: 0.578 ± 0.116
0.578CysIle: 0.578 ± 0.12
0.694CysLys: 0.694 ± 0.146
1.011CysLeu: 1.011 ± 0.212
0.087CysMet: 0.087 ± 0.056
0.694CysAsn: 0.694 ± 0.14
0.838CysPro: 0.838 ± 0.16
0.347CysGln: 0.347 ± 0.092
0.983CysArg: 0.983 ± 0.196
0.925CysSer: 0.925 ± 0.159
0.78CysThr: 0.78 ± 0.17
0.925CysVal: 0.925 ± 0.182
0.289CysTrp: 0.289 ± 0.084
0.462CysTyr: 0.462 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
5.606AspAla: 5.606 ± 0.453
1.098AspCys: 1.098 ± 0.193
4.393AspAsp: 4.393 ± 0.396
4.624AspGlu: 4.624 ± 0.419
2.081AspPhe: 2.081 ± 0.237
6.271AspGly: 6.271 ± 0.451
1.936AspHis: 1.936 ± 0.266
2.919AspIle: 2.919 ± 0.302
3.092AspLys: 3.092 ± 0.272
5.173AspLeu: 5.173 ± 0.408
1.474AspMet: 1.474 ± 0.195
2.167AspAsn: 2.167 ± 0.219
3.266AspPro: 3.266 ± 0.276
2.283AspGln: 2.283 ± 0.224
3.93AspArg: 3.93 ± 0.329
2.919AspSer: 2.919 ± 0.282
3.497AspThr: 3.497 ± 0.324
4.075AspVal: 4.075 ± 0.391
2.254AspTrp: 2.254 ± 0.265
2.283AspTyr: 2.283 ± 0.241
0.0AspXaa: 0.0 ± 0.0
Glu
6.676GluAla: 6.676 ± 0.486
1.474GluCys: 1.474 ± 0.251
4.248GluAsp: 4.248 ± 0.362
5.462GluGlu: 5.462 ± 0.514
2.89GluPhe: 2.89 ± 0.3
4.566GluGly: 4.566 ± 0.43
1.358GluHis: 1.358 ± 0.226
4.017GluIle: 4.017 ± 0.364
2.745GluLys: 2.745 ± 0.301
6.647GluLeu: 6.647 ± 0.507
2.543GluMet: 2.543 ± 0.27
1.618GluAsn: 1.618 ± 0.242
3.121GluPro: 3.121 ± 0.294
2.399GluGln: 2.399 ± 0.236
4.335GluArg: 4.335 ± 0.446
3.352GluSer: 3.352 ± 0.322
3.034GluThr: 3.034 ± 0.23
4.768GluVal: 4.768 ± 0.431
1.3GluTrp: 1.3 ± 0.218
2.745GluTyr: 2.745 ± 0.326
0.0GluXaa: 0.0 ± 0.0
Phe
2.485PheAla: 2.485 ± 0.264
0.491PheCys: 0.491 ± 0.121
2.543PheAsp: 2.543 ± 0.286
2.196PheGlu: 2.196 ± 0.238
1.127PhePhe: 1.127 ± 0.193
3.323PheGly: 3.323 ± 0.349
0.722PheHis: 0.722 ± 0.166
1.243PheIle: 1.243 ± 0.165
0.925PheLys: 0.925 ± 0.146
2.11PheLeu: 2.11 ± 0.248
0.636PheMet: 0.636 ± 0.125
1.358PheAsn: 1.358 ± 0.213
1.878PhePro: 1.878 ± 0.262
0.78PheGln: 0.78 ± 0.176
1.561PheArg: 1.561 ± 0.241
2.399PheSer: 2.399 ± 0.216
1.936PheThr: 1.936 ± 0.205
2.399PheVal: 2.399 ± 0.304
0.607PheTrp: 0.607 ± 0.147
0.896PheTyr: 0.896 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
7.485GlyAla: 7.485 ± 0.706
1.532GlyCys: 1.532 ± 0.226
5.404GlyAsp: 5.404 ± 0.46
6.184GlyGlu: 6.184 ± 0.426
2.977GlyPhe: 2.977 ± 0.301
9.334GlyGly: 9.334 ± 1.488
2.225GlyHis: 2.225 ± 0.249
3.757GlyIle: 3.757 ± 0.386
3.699GlyLys: 3.699 ± 0.365
7.08GlyLeu: 7.08 ± 0.586
2.138GlyMet: 2.138 ± 0.258
3.063GlyAsn: 3.063 ± 0.32
3.728GlyPro: 3.728 ± 0.376
2.543GlyGln: 2.543 ± 0.289
5.982GlyArg: 5.982 ± 0.408
4.999GlySer: 4.999 ± 0.566
4.942GlyThr: 4.942 ± 0.488
5.577GlyVal: 5.577 ± 0.471
2.052GlyTrp: 2.052 ± 0.252
3.526GlyTyr: 3.526 ± 0.275
0.0GlyXaa: 0.0 ± 0.0
His
2.138HisAla: 2.138 ± 0.232
0.491HisCys: 0.491 ± 0.126
1.561HisAsp: 1.561 ± 0.246
1.936HisGlu: 1.936 ± 0.23
0.578HisPhe: 0.578 ± 0.132
1.965HisGly: 1.965 ± 0.21
0.578HisHis: 0.578 ± 0.159
0.954HisIle: 0.954 ± 0.152
0.636HisLys: 0.636 ± 0.135
2.341HisLeu: 2.341 ± 0.275
0.405HisMet: 0.405 ± 0.125
0.491HisAsn: 0.491 ± 0.106
1.358HisPro: 1.358 ± 0.176
0.867HisGln: 0.867 ± 0.15
2.11HisArg: 2.11 ± 0.268
1.04HisSer: 1.04 ± 0.159
0.809HisThr: 0.809 ± 0.161
1.589HisVal: 1.589 ± 0.206
0.665HisTrp: 0.665 ± 0.133
0.78HisTyr: 0.78 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.751IleAla: 5.751 ± 0.405
0.462IleCys: 0.462 ± 0.129
3.497IleAsp: 3.497 ± 0.371
3.93IleGlu: 3.93 ± 0.317
1.214IlePhe: 1.214 ± 0.217
3.843IleGly: 3.843 ± 0.39
1.214IleHis: 1.214 ± 0.203
2.11IleIle: 2.11 ± 0.226
1.734IleLys: 1.734 ± 0.198
3.352IleLeu: 3.352 ± 0.359
0.954IleMet: 0.954 ± 0.186
1.792IleAsn: 1.792 ± 0.222
3.15IlePro: 3.15 ± 0.256
1.618IleGln: 1.618 ± 0.213
2.63IleArg: 2.63 ± 0.28
2.543IleSer: 2.543 ± 0.241
3.15IleThr: 3.15 ± 0.323
3.41IleVal: 3.41 ± 0.405
0.751IleTrp: 0.751 ± 0.154
1.676IleTyr: 1.676 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
4.161LysAla: 4.161 ± 0.346
0.578LysCys: 0.578 ± 0.12
1.705LysAsp: 1.705 ± 0.247
1.849LysGlu: 1.849 ± 0.28
1.329LysPhe: 1.329 ± 0.201
2.919LysGly: 2.919 ± 0.301
1.127LysHis: 1.127 ± 0.184
1.849LysIle: 1.849 ± 0.21
2.254LysLys: 2.254 ± 0.363
3.352LysLeu: 3.352 ± 0.364
1.734LysMet: 1.734 ± 0.223
0.694LysAsn: 0.694 ± 0.142
2.427LysPro: 2.427 ± 0.283
1.214LysGln: 1.214 ± 0.186
3.034LysArg: 3.034 ± 0.389
2.254LysSer: 2.254 ± 0.4
1.821LysThr: 1.821 ± 0.19
3.237LysVal: 3.237 ± 0.341
1.156LysTrp: 1.156 ± 0.185
1.272LysTyr: 1.272 ± 0.186
0.0LysXaa: 0.0 ± 0.0
Leu
8.294LeuAla: 8.294 ± 0.558
0.896LeuCys: 0.896 ± 0.194
5.693LeuAsp: 5.693 ± 0.409
4.826LeuGlu: 4.826 ± 0.432
1.849LeuPhe: 1.849 ± 0.228
6.907LeuGly: 6.907 ± 0.532
1.907LeuHis: 1.907 ± 0.229
3.468LeuIle: 3.468 ± 0.374
3.005LeuLys: 3.005 ± 0.33
5.606LeuLeu: 5.606 ± 0.439
2.052LeuMet: 2.052 ± 0.261
2.659LeuAsn: 2.659 ± 0.268
4.45LeuPro: 4.45 ± 0.343
2.341LeuGln: 2.341 ± 0.285
6.387LeuArg: 6.387 ± 0.488
4.826LeuSer: 4.826 ± 0.395
5.086LeuThr: 5.086 ± 0.367
4.971LeuVal: 4.971 ± 0.365
1.474LeuTrp: 1.474 ± 0.193
1.878LeuTyr: 1.878 ± 0.223
0.0LeuXaa: 0.0 ± 0.0
Met
1.994MetAla: 1.994 ± 0.264
0.173MetCys: 0.173 ± 0.068
1.214MetAsp: 1.214 ± 0.187
1.214MetGlu: 1.214 ± 0.213
0.636MetPhe: 0.636 ± 0.123
2.052MetGly: 2.052 ± 0.274
0.405MetHis: 0.405 ± 0.113
1.069MetIle: 1.069 ± 0.179
1.098MetLys: 1.098 ± 0.19
1.849MetLeu: 1.849 ± 0.217
0.607MetMet: 0.607 ± 0.125
1.474MetAsn: 1.474 ± 0.2
1.243MetPro: 1.243 ± 0.179
0.751MetGln: 0.751 ± 0.157
1.676MetArg: 1.676 ± 0.23
2.688MetSer: 2.688 ± 0.314
2.456MetThr: 2.456 ± 0.294
1.04MetVal: 1.04 ± 0.195
0.491MetTrp: 0.491 ± 0.125
0.636MetTyr: 0.636 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.266AsnAla: 3.266 ± 0.36
0.52AsnCys: 0.52 ± 0.12
2.11AsnAsp: 2.11 ± 0.245
1.705AsnGlu: 1.705 ± 0.247
0.925AsnPhe: 0.925 ± 0.163
3.757AsnGly: 3.757 ± 0.372
0.954AsnHis: 0.954 ± 0.158
1.329AsnIle: 1.329 ± 0.221
1.185AsnLys: 1.185 ± 0.204
2.659AsnLeu: 2.659 ± 0.292
0.636AsnMet: 0.636 ± 0.133
0.694AsnAsn: 0.694 ± 0.127
2.572AsnPro: 2.572 ± 0.278
1.011AsnGln: 1.011 ± 0.173
2.485AsnArg: 2.485 ± 0.246
1.532AsnSer: 1.532 ± 0.236
1.561AsnThr: 1.561 ± 0.2
2.138AsnVal: 2.138 ± 0.22
0.665AsnTrp: 0.665 ± 0.13
0.896AsnTyr: 0.896 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
5.115ProAla: 5.115 ± 0.412
0.867ProCys: 0.867 ± 0.178
3.208ProAsp: 3.208 ± 0.301
4.508ProGlu: 4.508 ± 0.406
1.821ProPhe: 1.821 ± 0.216
5.375ProGly: 5.375 ± 0.515
0.896ProHis: 0.896 ± 0.145
2.543ProIle: 2.543 ± 0.27
2.023ProLys: 2.023 ± 0.257
4.19ProLeu: 4.19 ± 0.345
1.127ProMet: 1.127 ± 0.172
1.965ProAsn: 1.965 ± 0.235
2.948ProPro: 2.948 ± 0.359
1.243ProGln: 1.243 ± 0.24
2.803ProArg: 2.803 ± 0.32
2.514ProSer: 2.514 ± 0.366
3.381ProThr: 3.381 ± 0.324
3.901ProVal: 3.901 ± 0.338
1.185ProTrp: 1.185 ± 0.21
1.445ProTyr: 1.445 ± 0.225
0.0ProXaa: 0.0 ± 0.0
Gln
3.092GlnAla: 3.092 ± 0.288
0.376GlnCys: 0.376 ± 0.118
1.561GlnAsp: 1.561 ± 0.223
1.878GlnGlu: 1.878 ± 0.276
1.04GlnPhe: 1.04 ± 0.206
1.907GlnGly: 1.907 ± 0.272
0.809GlnHis: 0.809 ± 0.132
1.387GlnIle: 1.387 ± 0.206
1.676GlnLys: 1.676 ± 0.245
2.572GlnLeu: 2.572 ± 0.311
1.069GlnMet: 1.069 ± 0.195
1.04GlnAsn: 1.04 ± 0.173
1.676GlnPro: 1.676 ± 0.198
1.156GlnGln: 1.156 ± 0.194
2.081GlnArg: 2.081 ± 0.226
1.994GlnSer: 1.994 ± 0.248
1.589GlnThr: 1.589 ± 0.209
1.907GlnVal: 1.907 ± 0.263
0.578GlnTrp: 0.578 ± 0.146
0.867GlnTyr: 0.867 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
6.3ArgAla: 6.3 ± 0.513
1.185ArgCys: 1.185 ± 0.18
4.219ArgAsp: 4.219 ± 0.351
4.855ArgGlu: 4.855 ± 0.493
2.341ArgPhe: 2.341 ± 0.277
5.52ArgGly: 5.52 ± 0.377
1.503ArgHis: 1.503 ± 0.199
3.872ArgIle: 3.872 ± 0.316
3.063ArgLys: 3.063 ± 0.311
5.115ArgLeu: 5.115 ± 0.352
2.023ArgMet: 2.023 ± 0.264
1.994ArgAsn: 1.994 ± 0.232
2.977ArgPro: 2.977 ± 0.307
2.254ArgGln: 2.254 ± 0.275
5.664ArgArg: 5.664 ± 0.551
2.601ArgSer: 2.601 ± 0.238
3.323ArgThr: 3.323 ± 0.423
4.537ArgVal: 4.537 ± 0.419
2.312ArgTrp: 2.312 ± 0.265
2.37ArgTyr: 2.37 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
5.491SerAla: 5.491 ± 0.469
0.954SerCys: 0.954 ± 0.146
3.381SerAsp: 3.381 ± 0.262
3.41SerGlu: 3.41 ± 0.294
1.358SerPhe: 1.358 ± 0.156
6.011SerGly: 6.011 ± 0.587
1.185SerHis: 1.185 ± 0.193
2.803SerIle: 2.803 ± 0.267
1.994SerLys: 1.994 ± 0.232
3.988SerLeu: 3.988 ± 0.339
1.127SerMet: 1.127 ± 0.174
1.763SerAsn: 1.763 ± 0.212
3.179SerPro: 3.179 ± 0.283
1.676SerGln: 1.676 ± 0.255
3.381SerArg: 3.381 ± 0.299
3.583SerSer: 3.583 ± 0.355
2.803SerThr: 2.803 ± 0.288
3.67SerVal: 3.67 ± 0.371
1.503SerTrp: 1.503 ± 0.187
1.272SerTyr: 1.272 ± 0.172
0.0SerXaa: 0.0 ± 0.0
Thr
5.202ThrAla: 5.202 ± 0.412
0.954ThrCys: 0.954 ± 0.146
3.468ThrAsp: 3.468 ± 0.36
3.381ThrGlu: 3.381 ± 0.38
2.254ThrPhe: 2.254 ± 0.229
5.173ThrGly: 5.173 ± 0.5
0.954ThrHis: 0.954 ± 0.182
3.208ThrIle: 3.208 ± 0.377
1.907ThrLys: 1.907 ± 0.253
4.421ThrLeu: 4.421 ± 0.369
0.838ThrMet: 0.838 ± 0.18
1.763ThrAsn: 1.763 ± 0.268
4.161ThrPro: 4.161 ± 0.427
1.387ThrGln: 1.387 ± 0.203
3.005ThrArg: 3.005 ± 0.268
2.456ThrSer: 2.456 ± 0.326
3.005ThrThr: 3.005 ± 0.371
4.682ThrVal: 4.682 ± 0.374
1.3ThrTrp: 1.3 ± 0.191
2.023ThrTyr: 2.023 ± 0.228
0.0ThrXaa: 0.0 ± 0.0
Val
6.733ValAla: 6.733 ± 0.487
0.954ValCys: 0.954 ± 0.168
5.057ValAsp: 5.057 ± 0.376
5.26ValGlu: 5.26 ± 0.471
1.994ValPhe: 1.994 ± 0.203
4.999ValGly: 4.999 ± 0.452
1.503ValHis: 1.503 ± 0.204
4.161ValIle: 4.161 ± 0.373
2.485ValLys: 2.485 ± 0.232
4.075ValLeu: 4.075 ± 0.409
1.416ValMet: 1.416 ± 0.179
2.601ValAsn: 2.601 ± 0.271
3.468ValPro: 3.468 ± 0.318
1.821ValGln: 1.821 ± 0.239
4.595ValArg: 4.595 ± 0.428
4.132ValSer: 4.132 ± 0.376
4.45ValThr: 4.45 ± 0.451
5.462ValVal: 5.462 ± 0.508
0.983ValTrp: 0.983 ± 0.162
2.11ValTyr: 2.11 ± 0.249
0.0ValXaa: 0.0 ± 0.0
Trp
2.023TrpAla: 2.023 ± 0.254
0.52TrpCys: 0.52 ± 0.126
1.387TrpAsp: 1.387 ± 0.21
1.069TrpGlu: 1.069 ± 0.164
0.722TrpPhe: 0.722 ± 0.139
1.503TrpGly: 1.503 ± 0.204
0.78TrpHis: 0.78 ± 0.151
0.954TrpIle: 0.954 ± 0.166
0.433TrpLys: 0.433 ± 0.104
1.792TrpLeu: 1.792 ± 0.243
0.491TrpMet: 0.491 ± 0.118
0.665TrpAsn: 0.665 ± 0.134
1.011TrpPro: 1.011 ± 0.179
0.838TrpGln: 0.838 ± 0.169
2.052TrpArg: 2.052 ± 0.259
1.878TrpSer: 1.878 ± 0.214
1.214TrpThr: 1.214 ± 0.212
1.936TrpVal: 1.936 ± 0.236
0.722TrpTrp: 0.722 ± 0.152
0.751TrpTyr: 0.751 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.41TyrAla: 3.41 ± 0.303
0.462TyrCys: 0.462 ± 0.132
2.456TyrAsp: 2.456 ± 0.297
2.11TyrGlu: 2.11 ± 0.222
0.867TyrPhe: 0.867 ± 0.152
3.294TyrGly: 3.294 ± 0.362
0.694TyrHis: 0.694 ± 0.141
1.185TyrIle: 1.185 ± 0.195
1.214TyrLys: 1.214 ± 0.188
2.659TyrLeu: 2.659 ± 0.358
0.289TyrMet: 0.289 ± 0.113
0.809TyrAsn: 0.809 ± 0.153
1.474TyrPro: 1.474 ± 0.206
1.04TyrGln: 1.04 ± 0.184
2.572TyrArg: 2.572 ± 0.303
1.589TyrSer: 1.589 ± 0.184
1.821TyrThr: 1.821 ± 0.233
2.225TyrVal: 2.225 ± 0.261
0.636TyrTrp: 0.636 ± 0.117
0.896TyrTyr: 0.896 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 241 proteins (34605 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski