Acinetobacter phage vB_AbaP_Acibel007
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075DXY1|A0A075DXY1_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaP_Acibel007 OX=1481187 GN=vB_AbaP_Acibel007_52 PE=4 SV=1
MM1 pKa = 7.83 FDD3 pKa = 3.83 RR4 pKa = 11.84 FHH6 pKa = 7.22 EE7 pKa = 4.76 DD8 pKa = 2.99 GEE10 pKa = 4.75 IIHH13 pKa = 6.4 EE14 pKa = 4.01 PVYY17 pKa = 10.91 LCNDD21 pKa = 3.63 CGEE24 pKa = 4.44 EE25 pKa = 4.33 SPHH28 pKa = 7.57 SEE30 pKa = 4.49 LFHH33 pKa = 6.4 ICCSEE38 pKa = 3.86 CDD40 pKa = 3.8 ALCPYY45 pKa = 10.38 CEE47 pKa = 4.34 SDD49 pKa = 5.32 DD50 pKa = 5.24 LEE52 pKa = 4.34 EE53 pKa = 4.15 LL54 pKa = 3.83
Molecular weight: 6.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.949
IPC2_protein 3.948
IPC_protein 3.846
Toseland 3.681
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.694
Grimsley 3.592
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 0.006
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A075DXY3|A0A075DXY3_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaP_Acibel007 OX=1481187 GN=vB_AbaP_Acibel007_15 PE=4 SV=1
MM1 pKa = 7.44 SNSIRR6 pKa = 11.84 IGRR9 pKa = 11.84 LTLNMGNVTVGVTRR23 pKa = 11.84 HH24 pKa = 4.98 QLSLFMQYY32 pKa = 10.16 SAYY35 pKa = 9.82 QSKK38 pKa = 9.75 FF39 pKa = 3.13
Molecular weight: 4.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 10.452
IPC_protein 11.242
Toseland 11.023
ProMoST 11.169
Dawson 11.14
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.111
Grimsley 11.213
Solomon 11.403
Lehninger 11.33
Nozaki 11.008
DTASelect 10.994
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.082
Patrickios 11.023
IPC_peptide 11.403
IPC2_peptide 10.248
IPC2.peptide.svr19 7.738
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
13310
39
1049
251.1
28.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.58 ± 0.352
1.142 ± 0.159
6.228 ± 0.2
5.8 ± 0.265
3.711 ± 0.153
7.077 ± 0.308
2.119 ± 0.137
5.327 ± 0.221
6.379 ± 0.293
8.64 ± 0.217
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.855 ± 0.176
5.049 ± 0.294
3.441 ± 0.151
4.688 ± 0.299
4.733 ± 0.193
5.725 ± 0.294
6.138 ± 0.277
7.107 ± 0.276
1.157 ± 0.082
4.102 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here