Bandicoot papillomatosis carcinomatosis virus type 2
Average proteome isoelectric point is 5.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3FN93|B3FN93_9PAPI Small t antigen OS=Bandicoot papillomatosis carcinomatosis virus type 2 OX=500654 GN=stag PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 4.52 NSDD5 pKa = 3.6 YY6 pKa = 11.67 LEE8 pKa = 3.98 FLEE11 pKa = 6.58 LIGCDD16 pKa = 3.34 TYY18 pKa = 11.57 DD19 pKa = 3.59 GPEE22 pKa = 4.2 NIKK25 pKa = 10.5 KK26 pKa = 10.21 KK27 pKa = 9.46 YY28 pKa = 9.95 RR29 pKa = 11.84 KK30 pKa = 9.23 AALKK34 pKa = 9.58 NHH36 pKa = 6.73 PDD38 pKa = 3.31 KK39 pKa = 11.47 GGSANKK45 pKa = 9.24 MKK47 pKa = 10.64 RR48 pKa = 11.84 LNEE51 pKa = 3.74 LNQIYY56 pKa = 7.84 TEE58 pKa = 4.62 CGPPQNKK65 pKa = 9.26 RR66 pKa = 11.84 YY67 pKa = 9.58 RR68 pKa = 11.84 PSSHH72 pKa = 7.04 YY73 pKa = 10.47 EE74 pKa = 3.83 EE75 pKa = 5.59 EE76 pKa = 5.04 DD77 pKa = 3.55 LFCYY81 pKa = 10.43 EE82 pKa = 4.92 SGGEE86 pKa = 4.49 DD87 pKa = 3.77 EE88 pKa = 6.13 PDD90 pKa = 3.14 TCSYY94 pKa = 10.69 NRR96 pKa = 11.84 SDD98 pKa = 3.87 SGFSEE103 pKa = 4.59 GNYY106 pKa = 6.55 TTPDD110 pKa = 3.38 PSGFSASNVSFSQSCRR126 pKa = 11.84 NVKK129 pKa = 10.2 LLLDD133 pKa = 3.36 ITEE136 pKa = 4.37 TLGHH140 pKa = 6.31 GRR142 pKa = 11.84 HH143 pKa = 6.12 CAHH146 pKa = 6.61 TLEE149 pKa = 6.3 AIDD152 pKa = 5.28 KK153 pKa = 8.49 NNKK156 pKa = 8.35 MDD158 pKa = 3.49 WEE160 pKa = 4.35 LFDD163 pKa = 4.47 TFCATPGNHH172 pKa = 7.08 HH173 pKa = 6.31 LTIMFIEE180 pKa = 4.34 HH181 pKa = 7.39 CYY183 pKa = 9.98 MKK185 pKa = 10.81 LKK187 pKa = 10.35 DD188 pKa = 3.95 WQWSLMNFVNYY199 pKa = 9.74 GPSYY203 pKa = 10.75 LEE205 pKa = 4.36 EE206 pKa = 4.48 IKK208 pKa = 10.74 EE209 pKa = 3.89 AANAINWSQLDD220 pKa = 3.67 DD221 pKa = 4.18 MIFSS225 pKa = 4.14
Molecular weight: 25.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.716
IPC2_protein 4.876
IPC_protein 4.825
Toseland 4.736
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.673
Rodwell 4.711
Grimsley 4.647
Solomon 4.8
Lehninger 4.762
Nozaki 4.914
DTASelect 5.08
Thurlkill 4.736
EMBOSS 4.711
Sillero 4.991
Patrickios 3.147
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 4.945
Protein with the highest isoelectric point:
>tr|B3FN93|B3FN93_9PAPI Small t antigen OS=Bandicoot papillomatosis carcinomatosis virus type 2 OX=500654 GN=stag PE=4 SV=1
MM1 pKa = 7.93 AIWQPNGKK9 pKa = 9.93 VYY11 pKa = 10.68 LPPSAPVARR20 pKa = 11.84 ILNTEE25 pKa = 4.21 EE26 pKa = 3.93 YY27 pKa = 9.77 IQRR30 pKa = 11.84 TNLFYY35 pKa = 10.66 HH36 pKa = 6.99 ANTEE40 pKa = 3.95 RR41 pKa = 11.84 LLTVGHH47 pKa = 7.43 PYY49 pKa = 10.94 YY50 pKa = 10.38 EE51 pKa = 4.34 IKK53 pKa = 10.89 DD54 pKa = 3.69 NLDD57 pKa = 3.3 PSKK60 pKa = 10.77 IGVPKK65 pKa = 10.6 VSANQYY71 pKa = 8.75 RR72 pKa = 11.84 VFRR75 pKa = 11.84 IKK77 pKa = 10.92 LPDD80 pKa = 4.01 PNRR83 pKa = 11.84 FALGDD88 pKa = 3.44 KK89 pKa = 10.45 SLYY92 pKa = 10.63 DD93 pKa = 3.64 PEE95 pKa = 4.66 KK96 pKa = 10.7 EE97 pKa = 3.83 RR98 pKa = 11.84 LVWGVRR104 pKa = 11.84 GLEE107 pKa = 4.05 IEE109 pKa = 4.3 RR110 pKa = 11.84 GQPLNVGSSGNVLYY124 pKa = 11.19 NKK126 pKa = 10.15 FKK128 pKa = 10.46 DD129 pKa = 3.7 TEE131 pKa = 4.25 NPNNYY136 pKa = 9.68 AKK138 pKa = 10.57 EE139 pKa = 4.47 GEE141 pKa = 4.28 DD142 pKa = 3.25 EE143 pKa = 4.24 RR144 pKa = 11.84 QNVSHH149 pKa = 7.51 DD150 pKa = 3.78 PKK152 pKa = 10.69 QMQMLLVGCKK162 pKa = 9.37 PATGEE167 pKa = 3.82 HH168 pKa = 6.02 WDD170 pKa = 3.59 AAKK173 pKa = 9.44 TCAEE177 pKa = 4.09 RR178 pKa = 11.84 PPNKK182 pKa = 9.82 GDD184 pKa = 3.8 CPPLEE189 pKa = 4.32 LVNSIIEE196 pKa = 4.65 DD197 pKa = 3.37 GDD199 pKa = 4.03 MIDD202 pKa = 4.24 IGYY205 pKa = 10.86 GNLNFKK211 pKa = 7.55 TLCGNKK217 pKa = 9.67 SDD219 pKa = 4.25 VPLDD223 pKa = 3.35 INTTTTKK230 pKa = 10.77 YY231 pKa = 10.55 PDD233 pKa = 3.9 FLKK236 pKa = 8.54 MTSDD240 pKa = 3.44 QYY242 pKa = 11.69 GDD244 pKa = 3.6 SLFFYY249 pKa = 9.09 TKK251 pKa = 10.34 RR252 pKa = 11.84 EE253 pKa = 3.93 STYY256 pKa = 11.15 ARR258 pKa = 11.84 HH259 pKa = 5.5 FWTRR263 pKa = 11.84 GGTPGDD269 pKa = 4.73 PIPEE273 pKa = 4.0 EE274 pKa = 4.64 LFVNPSDD281 pKa = 3.66 NQKK284 pKa = 10.0 ILGSSTYY291 pKa = 8.56 FTVPSGSLVSSEE303 pKa = 3.88 SQLFNRR309 pKa = 11.84 PYY311 pKa = 9.9 WLQKK315 pKa = 10.1 ATGQNNGICWNNDD328 pKa = 2.76 LFITVVDD335 pKa = 3.59 NTRR338 pKa = 11.84 NCNFTISVFNKK349 pKa = 9.54 ADD351 pKa = 3.35 SSQGQTYY358 pKa = 10.26 KK359 pKa = 10.75 SSNYY363 pKa = 10.51 NMFLRR368 pKa = 11.84 HH369 pKa = 5.36 VEE371 pKa = 3.94 EE372 pKa = 4.95 FEE374 pKa = 4.85 LNFIFEE380 pKa = 4.24 ICKK383 pKa = 10.15 VPLKK387 pKa = 10.77 ADD389 pKa = 3.45 VLAHH393 pKa = 6.42 INAMDD398 pKa = 3.85 PRR400 pKa = 11.84 ILEE403 pKa = 4.24 DD404 pKa = 3.02 WSLGFVPSNNFPLEE418 pKa = 4.04 DD419 pKa = 3.31 KK420 pKa = 11.15 YY421 pKa = 11.57 RR422 pKa = 11.84 FINSLATRR430 pKa = 11.84 CPDD433 pKa = 3.48 KK434 pKa = 10.29 EE435 pKa = 4.59 TPKK438 pKa = 10.66 EE439 pKa = 3.94 KK440 pKa = 10.11 EE441 pKa = 3.59 DD442 pKa = 3.89 PYY444 pKa = 10.97 RR445 pKa = 11.84 SNTFWTVEE453 pKa = 3.82 LEE455 pKa = 3.97 EE456 pKa = 4.57 RR457 pKa = 11.84 MSSEE461 pKa = 3.91 LDD463 pKa = 3.2 QYY465 pKa = 11.36 PLGRR469 pKa = 11.84 KK470 pKa = 8.67 FLFQSNISSINTVAKK485 pKa = 9.24 PRR487 pKa = 11.84 KK488 pKa = 9.31 RR489 pKa = 11.84 KK490 pKa = 8.58 ATTPTTKK497 pKa = 9.76 TKK499 pKa = 10.2 RR500 pKa = 11.84 RR501 pKa = 11.84 KK502 pKa = 9.36
Molecular weight: 57.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.395
IPC2_protein 6.517
IPC_protein 6.605
Toseland 6.576
ProMoST 7.132
Dawson 7.102
Bjellqvist 7.044
Wikipedia 7.059
Rodwell 7.102
Grimsley 6.649
Solomon 7.132
Lehninger 7.146
Nozaki 7.351
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.439
Sillero 7.512
Patrickios 4.279
IPC_peptide 7.132
IPC2_peptide 6.883
IPC2.peptide.svr19 6.869
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1196
225
502
398.7
44.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.264 ± 0.137
1.505 ± 0.583
6.689 ± 0.359
6.773 ± 0.428
4.515 ± 0.24
6.773 ± 0.707
1.672 ± 0.478
6.187 ± 1.033
5.769 ± 0.871
7.776 ± 0.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.756 ± 0.347
6.856 ± 0.869
7.191 ± 0.758
2.926 ± 0.188
5.1 ± 0.506
7.609 ± 0.412
6.438 ± 0.391
5.435 ± 1.185
1.003 ± 0.442
3.763 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here