Wuhan aphid virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0QL38|A0A0P0QL38_9VIRU Uncharacterized protein OS=Wuhan aphid virus 1 OX=1746067 GN=VP4 PE=4 SV=1
MM1 pKa = 7.64IAQGLLSLMLTATLATSLRR20 pKa = 11.84DD21 pKa = 3.5YY22 pKa = 10.9MRR24 pKa = 11.84DD25 pKa = 3.16EE26 pKa = 4.21NTLVICSEE34 pKa = 4.28YY35 pKa = 10.69SEE37 pKa = 4.29PWRR40 pKa = 11.84ISMCASSQLKK50 pKa = 10.64DD51 pKa = 3.68EE52 pKa = 5.54LISTHH57 pKa = 6.2TYY59 pKa = 7.74ITYY62 pKa = 9.98LQDD65 pKa = 4.77AMALLPGHH73 pKa = 6.28SKK75 pKa = 8.77YY76 pKa = 9.54TSIGVTTGHH85 pKa = 7.47DD86 pKa = 3.21KK87 pKa = 10.98RR88 pKa = 11.84IVLGQQLHH96 pKa = 6.01AHH98 pKa = 6.23FNWAYY103 pKa = 8.08EE104 pKa = 4.12AKK106 pKa = 9.93YY107 pKa = 10.53DD108 pKa = 3.91PRR110 pKa = 11.84DD111 pKa = 3.55YY112 pKa = 10.89YY113 pKa = 10.68TDD115 pKa = 3.38KK116 pKa = 10.85TFLAMRR122 pKa = 11.84YY123 pKa = 7.24KK124 pKa = 9.75TEE126 pKa = 3.98RR127 pKa = 11.84PILLFTVNYY136 pKa = 9.18EE137 pKa = 4.14PEE139 pKa = 4.0VPEE142 pKa = 4.08WNEE145 pKa = 3.87KK146 pKa = 8.86YY147 pKa = 10.51ISMLQPGGEE156 pKa = 3.94KK157 pKa = 10.26GAPRR161 pKa = 11.84LYY163 pKa = 9.91TGKK166 pKa = 10.59GYY168 pKa = 10.47RR169 pKa = 11.84ALTGKK174 pKa = 7.75QTEE177 pKa = 4.67DD178 pKa = 3.29CTSFYY183 pKa = 11.3DD184 pKa = 3.21WDD186 pKa = 3.59QRR188 pKa = 11.84EE189 pKa = 4.37SYY191 pKa = 8.09EE192 pKa = 4.28TAKK195 pKa = 9.44NTAILMDD202 pKa = 4.51PKK204 pKa = 10.37LWCPIIKK211 pKa = 10.15ASFEE215 pKa = 4.0IVVAEE220 pKa = 4.22IRR222 pKa = 11.84KK223 pKa = 5.91TTINTPHH230 pKa = 6.57GKK232 pKa = 7.7EE233 pKa = 4.09TIYY236 pKa = 9.91EE237 pKa = 4.55TVWEE241 pKa = 4.23NAGGRR246 pKa = 11.84LFGEE250 pKa = 4.85VPKK253 pKa = 10.56KK254 pKa = 9.72WSTVFLL260 pKa = 4.35

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0QL38|A0A0P0QL38_9VIRU Uncharacterized protein OS=Wuhan aphid virus 1 OX=1746067 GN=VP4 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.37ALYY5 pKa = 10.68LNAFCLFLIVCSTNAAQTTAAMNDD29 pKa = 3.93LEE31 pKa = 4.64SAWKK35 pKa = 9.92FDD37 pKa = 3.42LHH39 pKa = 6.12GFSVNPEE46 pKa = 3.7RR47 pKa = 11.84WDD49 pKa = 3.37DD50 pKa = 3.67ALRR53 pKa = 11.84LRR55 pKa = 11.84MYY57 pKa = 10.6QVGTAAAYY65 pKa = 8.74AATLPCSLPHH75 pKa = 6.27TLMFALVMFGMVRR88 pKa = 11.84LSWLLVAASCLALHH102 pKa = 7.01GYY104 pKa = 8.32KK105 pKa = 10.26RR106 pKa = 11.84KK107 pKa = 9.78NRR109 pKa = 11.84VFLGIGLALAYY120 pKa = 10.32ALAFGTKK127 pKa = 10.55LII129 pKa = 4.5

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2993

129

907

498.8

56.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.148 ± 0.857

1.437 ± 0.268

4.911 ± 0.501

4.945 ± 0.44

4.277 ± 0.229

7.016 ± 0.492

2.74 ± 0.354

6.682 ± 0.4

5.179 ± 0.392

10.057 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.408 ± 0.245

3.775 ± 0.367

4.611 ± 0.14

3.508 ± 0.222

5.446 ± 0.299

5.78 ± 0.252

7.584 ± 0.607

5.981 ± 0.294

1.871 ± 0.297

4.577 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski