Trypanosoma cruzi Dm28c

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum; Trypanosoma cruzi

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5B519|V5B519_TRYCR Uncharacterized protein OS=Trypanosoma cruzi Dm28c OX=1416333 GN=TCDM_12245 PE=4 SV=1
MM1 pKa = 6.98MCEE4 pKa = 3.85EE5 pKa = 5.26CFVDD9 pKa = 3.23WWNSKK14 pKa = 8.93IYY16 pKa = 10.06IYY18 pKa = 10.26IFCEE22 pKa = 3.46VHH24 pKa = 5.73YY25 pKa = 10.05LYY27 pKa = 7.78TTSCFYY33 pKa = 10.99FPACNMKK40 pKa = 10.66LKK42 pKa = 10.21FFWIDD47 pKa = 3.23FSLFFWDD54 pKa = 4.62FCRR57 pKa = 11.84SVNFLFFFLVAFIGLMFLMKK77 pKa = 10.56CMLLPLVFFTAWLCDD92 pKa = 3.46LSLFLLSLCVDD103 pKa = 3.75VLLVCAEE110 pKa = 4.1GCTQVTGVMAMMMTGRR126 pKa = 11.84VLLVCALCVLWCGAGGRR143 pKa = 11.84CDD145 pKa = 3.8EE146 pKa = 5.03GEE148 pKa = 4.11DD149 pKa = 3.78TAGSGSGGEE158 pKa = 4.2HH159 pKa = 6.32SLASKK164 pKa = 10.39QLEE167 pKa = 4.15NSSQDD172 pKa = 3.49TQSLKK177 pKa = 11.04GGVPGAKK184 pKa = 10.03GNVPPAPTDD193 pKa = 3.64EE194 pKa = 4.97EE195 pKa = 6.34DD196 pKa = 4.63DD197 pKa = 5.83DD198 pKa = 4.93SDD200 pKa = 4.02NDD202 pKa = 3.5EE203 pKa = 5.02DD204 pKa = 3.95EE205 pKa = 4.41GTEE208 pKa = 4.14VEE210 pKa = 4.53EE211 pKa = 4.32KK212 pKa = 10.48TIGRR216 pKa = 11.84QGGQGGTTVPGPDD229 pKa = 3.2STEE232 pKa = 3.86TNLIGTEE239 pKa = 4.02QQTGQSIISAEE250 pKa = 4.36EE251 pKa = 3.79IPPSGSQEE259 pKa = 3.79PKK261 pKa = 10.77VILTQPEE268 pKa = 4.27VEE270 pKa = 4.71GKK272 pKa = 10.24NDD274 pKa = 3.6TDD276 pKa = 3.4KK277 pKa = 11.44SRR279 pKa = 11.84PAVEE283 pKa = 4.21NALTTVNGEE292 pKa = 3.81NTLPGGVNLPSPPEE306 pKa = 4.46DD307 pKa = 3.4GVDD310 pKa = 3.2SHH312 pKa = 6.12EE313 pKa = 4.39QSGEE317 pKa = 3.95DD318 pKa = 3.3TTSEE322 pKa = 4.09DD323 pKa = 3.84EE324 pKa = 4.59KK325 pKa = 11.16NVPSPEE331 pKa = 3.97TAATPQSHH339 pKa = 6.56RR340 pKa = 11.84GEE342 pKa = 4.4GSEE345 pKa = 4.57GTGEE349 pKa = 4.0DD350 pKa = 3.46TKK352 pKa = 11.26ATTLTANTTDD362 pKa = 3.32TTNTQNSDD370 pKa = 3.0SSTVKK375 pKa = 10.14MNEE378 pKa = 3.76AAPQTTITLTAAQTNHH394 pKa = 5.27TVTTGNSDD402 pKa = 2.8GSTAVSHH409 pKa = 5.47TTSSLLLLLLFACAAAAAVVAAA431 pKa = 4.95

Molecular weight: 45.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5AN66|V5AN66_TRYCR Uncharacterized protein OS=Trypanosoma cruzi Dm28c OX=1416333 GN=TCDM_13482 PE=4 SV=1
MM1 pKa = 7.4HH2 pKa = 7.41KK3 pKa = 10.12KK4 pKa = 9.87KK5 pKa = 10.19KK6 pKa = 9.58RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.41RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 10.33RR15 pKa = 11.84NNKK18 pKa = 9.66RR19 pKa = 11.84ILRR22 pKa = 11.84MKK24 pKa = 10.07TKK26 pKa = 10.53TNTKK30 pKa = 9.39KK31 pKa = 10.69RR32 pKa = 11.84KK33 pKa = 6.05TKK35 pKa = 9.37KK36 pKa = 9.5RR37 pKa = 11.84KK38 pKa = 9.28RR39 pKa = 11.84KK40 pKa = 9.4RR41 pKa = 11.84KK42 pKa = 8.36RR43 pKa = 11.84KK44 pKa = 8.12GMKK47 pKa = 9.65RR48 pKa = 11.84KK49 pKa = 7.56TEE51 pKa = 3.72GMVRR55 pKa = 11.84MKK57 pKa = 10.69RR58 pKa = 11.84GIQRR62 pKa = 11.84KK63 pKa = 9.01KK64 pKa = 10.6KK65 pKa = 10.3LLPTPQRR72 pKa = 3.52

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11346

0

11346

5280116

51

4838

465.4

51.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.477 ± 0.024

2.255 ± 0.014

4.732 ± 0.013

6.823 ± 0.029

3.736 ± 0.015

6.73 ± 0.026

2.677 ± 0.011

3.792 ± 0.014

4.416 ± 0.022

9.562 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.007

3.542 ± 0.011

4.965 ± 0.018

3.745 ± 0.018

7.083 ± 0.023

8.009 ± 0.025

5.872 ± 0.016

7.476 ± 0.028

1.249 ± 0.007

2.326 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski