Bacteroides pyogenes JCM 6292
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3340 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W4P4Z1|W4P4Z1_9BACE Mucin-desulfating sulfatase OS=Bacteroides pyogenes JCM 6292 OX=1235809 GN=JCM6292_1010 PE=3 SV=1
MM1 pKa = 7.31 MDD3 pKa = 3.26 YY4 pKa = 11.02 RR5 pKa = 11.84 LIYY8 pKa = 9.85 CLRR11 pKa = 11.84 NGLPLDD17 pKa = 3.49 MDD19 pKa = 4.14 VYY21 pKa = 11.27 DD22 pKa = 4.0 AAEE25 pKa = 4.05 WSCITEE31 pKa = 4.04 LSEE34 pKa = 4.41 QSVLQGSIPVAIPDD48 pKa = 4.0 FTRR51 pKa = 11.84 GAIWPDD57 pKa = 3.17 NPP59 pKa = 4.21
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.884
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|W4PCU1|W4PCU1_9BACE Uncharacterized protein OS=Bacteroides pyogenes JCM 6292 OX=1235809 GN=JCM6292_3513 PE=4 SV=1
MM1 pKa = 7.55 SCLSCRR7 pKa = 11.84 IKK9 pKa = 10.65 ASHH12 pKa = 6.31 SSYY15 pKa = 10.42 VSSIVFGRR23 pKa = 11.84 RR24 pKa = 11.84 LSLVCFLSHH33 pKa = 5.91 GHH35 pKa = 5.21 FTRR38 pKa = 11.84 SSSRR42 pKa = 11.84 MSRR45 pKa = 11.84 KK46 pKa = 9.31 RR47 pKa = 11.84 PKK49 pKa = 10.68 ASICSCLVCFIIYY62 pKa = 9.25 YY63 pKa = 8.94 FFVIFLFSMQILFLSRR79 pKa = 11.84 AKK81 pKa = 10.17 ILQIWEE87 pKa = 4.44 RR88 pKa = 11.84 KK89 pKa = 9.46 RR90 pKa = 11.84 EE91 pKa = 3.93 MGKK94 pKa = 10.2
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.687
ProMoST 10.423
Dawson 10.789
Bjellqvist 10.511
Wikipedia 10.979
Rodwell 11.067
Grimsley 10.833
Solomon 10.891
Lehninger 10.862
Nozaki 10.716
DTASelect 10.482
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.73
Patrickios 10.818
IPC_peptide 10.891
IPC2_peptide 9.882
IPC2.peptide.svr19 8.427
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3340
0
3340
982162
38
1903
294.1
33.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.307 ± 0.039
1.214 ± 0.017
5.335 ± 0.033
6.761 ± 0.044
4.634 ± 0.027
6.905 ± 0.039
1.908 ± 0.02
6.691 ± 0.04
6.823 ± 0.041
9.066 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.781 ± 0.018
4.867 ± 0.041
3.8 ± 0.03
3.241 ± 0.026
5.175 ± 0.037
6.143 ± 0.035
5.425 ± 0.031
6.425 ± 0.038
1.208 ± 0.017
4.292 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here