Sewage-associated circular DNA virus-19

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI41|A0A0B4UI41_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-19 OX=1592086 PE=4 SV=1
MM1 pKa = 6.8SQARR5 pKa = 11.84FWILTIPHH13 pKa = 7.26ADD15 pKa = 3.51FTPYY19 pKa = 10.68LPPNVNHH26 pKa = 7.34ISGQLEE32 pKa = 4.1RR33 pKa = 11.84GTGLNQYY40 pKa = 7.37LHH42 pKa = 5.49WQIVVSFARR51 pKa = 11.84KK52 pKa = 8.88IRR54 pKa = 11.84LGGVKK59 pKa = 10.2SIFGDD64 pKa = 3.57SCHH67 pKa = 7.09AEE69 pKa = 4.11PTRR72 pKa = 11.84SDD74 pKa = 3.12AARR77 pKa = 11.84KK78 pKa = 8.88YY79 pKa = 7.54VHH81 pKa = 6.93KK82 pKa = 10.57KK83 pKa = 7.57DD84 pKa = 3.5TRR86 pKa = 11.84VDD88 pKa = 3.38GTGFEE93 pKa = 5.19LGTLPIRR100 pKa = 11.84RR101 pKa = 11.84GEE103 pKa = 4.2SCDD106 pKa = 3.2WEE108 pKa = 4.25AVRR111 pKa = 11.84EE112 pKa = 4.0NAKK115 pKa = 10.1RR116 pKa = 11.84GRR118 pKa = 11.84MDD120 pKa = 6.55DD121 pKa = 3.18IPADD125 pKa = 3.69IFCRR129 pKa = 11.84LYY131 pKa = 11.52GNLKK135 pKa = 10.23RR136 pKa = 11.84IAVDD140 pKa = 3.15NMAPIGLEE148 pKa = 3.72RR149 pKa = 11.84TINVYY154 pKa = 9.07WGRR157 pKa = 11.84TGTGKK162 pKa = 10.01SRR164 pKa = 11.84RR165 pKa = 11.84AWAEE169 pKa = 3.3AGMDD173 pKa = 4.61AFPKK177 pKa = 10.51DD178 pKa = 3.77PRR180 pKa = 11.84TKK182 pKa = 9.94FWDD185 pKa = 4.39GYY187 pKa = 8.38RR188 pKa = 11.84SHH190 pKa = 6.73RR191 pKa = 11.84HH192 pKa = 3.57VVIDD196 pKa = 3.79EE197 pKa = 4.0FRR199 pKa = 11.84GGIDD203 pKa = 3.25VAHH206 pKa = 7.59LLRR209 pKa = 11.84WFDD212 pKa = 3.74RR213 pKa = 11.84YY214 pKa = 10.05PVVVEE219 pKa = 4.03VKK221 pKa = 10.34GSSVVLAAEE230 pKa = 4.51TVWITSNLDD239 pKa = 3.01PRR241 pKa = 11.84QWYY244 pKa = 8.69PDD246 pKa = 3.61LDD248 pKa = 4.18EE249 pKa = 4.59EE250 pKa = 4.49TLAALLRR257 pKa = 11.84RR258 pKa = 11.84LNITHH263 pKa = 6.07FTII266 pKa = 4.86

Molecular weight: 30.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI41|A0A0B4UI41_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-19 OX=1592086 PE=4 SV=1
MM1 pKa = 7.16SVVRR5 pKa = 11.84KK6 pKa = 8.61RR7 pKa = 11.84TGGYY11 pKa = 5.71TTYY14 pKa = 11.3APNKK18 pKa = 9.1KK19 pKa = 8.59MRR21 pKa = 11.84SGRR24 pKa = 11.84DD25 pKa = 3.15ILVSAGLAASRR36 pKa = 11.84KK37 pKa = 8.04YY38 pKa = 10.69GPRR41 pKa = 11.84LVSMAAQKK49 pKa = 9.93LASMAYY55 pKa = 9.8AAAKK59 pKa = 9.89DD60 pKa = 3.77DD61 pKa = 5.53PITQFRR67 pKa = 11.84NAKK70 pKa = 9.47KK71 pKa = 8.16RR72 pKa = 11.84TKK74 pKa = 9.92GRR76 pKa = 11.84SYY78 pKa = 11.28SEE80 pKa = 4.1QKK82 pKa = 10.59GVAAGKK88 pKa = 9.75FRR90 pKa = 11.84ATKK93 pKa = 10.36RR94 pKa = 11.84SPKK97 pKa = 10.35NIWLKK102 pKa = 7.72YY103 pKa = 6.6TRR105 pKa = 11.84KK106 pKa = 10.31GCVTTKK112 pKa = 10.37EE113 pKa = 4.29HH114 pKa = 6.32GCVLTGTVNTNAIYY128 pKa = 10.18FGHH131 pKa = 5.78STHH134 pKa = 7.04ANTYY138 pKa = 10.89LMLTVYY144 pKa = 9.48MRR146 pKa = 11.84AIVIHH151 pKa = 6.6IFRR154 pKa = 11.84KK155 pKa = 10.23GGVQIEE161 pKa = 4.65DD162 pKa = 4.12IEE164 pKa = 4.61TEE166 pKa = 4.31SPGSGGSMTIEE177 pKa = 3.73YY178 pKa = 8.73TYY180 pKa = 10.4NTDD183 pKa = 2.76IANQFVTVAFTGGLTLNQIAGSFASQFTNILFNNANDD220 pKa = 3.68EE221 pKa = 4.18QRR223 pKa = 11.84FFPILEE229 pKa = 4.27AKK231 pKa = 10.21IEE233 pKa = 4.06IPNLEE238 pKa = 4.56LCKK241 pKa = 9.93QDD243 pKa = 3.32YY244 pKa = 10.26RR245 pKa = 11.84NSIVHH250 pKa = 5.94VMVKK254 pKa = 10.38SDD256 pKa = 4.09LKK258 pKa = 9.95VQNRR262 pKa = 11.84TLATGTDD269 pKa = 3.45EE270 pKa = 5.82DD271 pKa = 4.59NNSSEE276 pKa = 4.85NIANQPLYY284 pKa = 11.16GKK286 pKa = 10.05FYY288 pKa = 9.59HH289 pKa = 6.78GKK291 pKa = 9.65GNGVFARR298 pKa = 11.84ANAGAGTGKK307 pKa = 10.57SITAGCSSGLIAYY320 pKa = 7.74APPAGALWLRR330 pKa = 11.84EE331 pKa = 4.02PPLPQLFTPMPKK343 pKa = 9.94HH344 pKa = 6.29NKK346 pKa = 9.49ALLNPGEE353 pKa = 4.3VKK355 pKa = 10.18TNSLLATYY363 pKa = 9.94RR364 pKa = 11.84VRR366 pKa = 11.84NLDD369 pKa = 3.43FFKK372 pKa = 11.18SLFFGASTSLAKK384 pKa = 10.21DD385 pKa = 2.93VHH387 pKa = 5.84VQHH390 pKa = 7.08KK391 pKa = 9.34FGKK394 pKa = 8.53YY395 pKa = 9.82CFFALEE401 pKa = 4.05KK402 pKa = 9.77MLTVSAADD410 pKa = 3.56SSPQVGLEE418 pKa = 3.63LNQRR422 pKa = 11.84VGVMFTFPRR431 pKa = 11.84NTDD434 pKa = 3.02TAEE437 pKa = 3.84ITEE440 pKa = 4.48VPSFITPLTTT450 pKa = 3.83

Molecular weight: 49.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

716

266

450

358.0

39.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.799 ± 0.757

1.117 ± 0.006

4.609 ± 1.498

4.33 ± 0.33

4.888 ± 0.445

7.961 ± 0.039

2.514 ± 0.514

5.447 ± 0.558

6.006 ± 1.106

7.542 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.35

5.168 ± 1.055

4.609 ± 0.165

2.793 ± 0.54

6.983 ± 1.872

5.866 ± 0.801

7.821 ± 1.068

6.425 ± 0.425

1.536 ± 1.092

3.492 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski