Diversispora epigaea
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397J556|A0A397J556_9GLOM Uncharacterized protein OS=Diversispora epigaea OX=1348612 GN=Glove_114g88 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 6.24 KK3 pKa = 10.22 IQHH6 pKa = 6.24 IKK8 pKa = 7.65 THH10 pKa = 5.83 SADD13 pKa = 3.67 TISRR17 pKa = 11.84 FTNLQIQTIIDD28 pKa = 3.84 HH29 pKa = 5.68 FTEE32 pKa = 4.35 KK33 pKa = 10.8 PNMEE37 pKa = 4.27 FTDD40 pKa = 4.62 DD41 pKa = 4.22 PSDD44 pKa = 3.71 GLPEE48 pKa = 4.03 IKK50 pKa = 10.72 ANTSYY55 pKa = 10.12 PAPKK59 pKa = 9.87 TYY61 pKa = 10.38 ISCSNSDD68 pKa = 3.91 FSDD71 pKa = 5.29 DD72 pKa = 5.05 DD73 pKa = 6.03 DD74 pKa = 6.32 SDD76 pKa = 4.7 NDD78 pKa = 3.8 DD79 pKa = 5.58 DD80 pKa = 4.66 EE81 pKa = 7.17 ANDD84 pKa = 3.46 MSVTYY89 pKa = 10.49 FDD91 pKa = 5.87 DD92 pKa = 3.71 EE93 pKa = 4.4 AEE95 pKa = 4.22 YY96 pKa = 11.04 YY97 pKa = 11.07 YY98 pKa = 11.4 NLDD101 pKa = 3.62 GGNLL105 pKa = 3.49
Molecular weight: 11.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.567
Grimsley 3.414
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A397HCE2|A0A397HCE2_9GLOM Uncharacterized protein OS=Diversispora epigaea OX=1348612 GN=Glove_353g27 PE=4 SV=1
MM1 pKa = 8.02 PKK3 pKa = 10.05 RR4 pKa = 11.84 KK5 pKa = 9.46 NLEE8 pKa = 4.09 NPTPAPLRR16 pKa = 11.84 RR17 pKa = 11.84 SPRR20 pKa = 11.84 IAARR24 pKa = 11.84 VAAQQLTPTPGRR36 pKa = 11.84 PPKK39 pKa = 10.04 RR40 pKa = 11.84 GRR42 pKa = 11.84 GRR44 pKa = 11.84 PRR46 pKa = 11.84 KK47 pKa = 7.36 NTRR50 pKa = 11.84 IAPGKK55 pKa = 9.96
Molecular weight: 6.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28108
0
28108
10124451
49
10082
360.2
41.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.137 ± 0.011
1.549 ± 0.006
5.479 ± 0.01
7.371 ± 0.017
4.558 ± 0.011
4.438 ± 0.017
2.168 ± 0.006
8.327 ± 0.015
8.25 ± 0.019
8.671 ± 0.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.924 ± 0.006
7.861 ± 0.021
3.871 ± 0.014
3.763 ± 0.011
4.351 ± 0.011
7.941 ± 0.018
5.495 ± 0.015
4.501 ± 0.012
1.306 ± 0.005
3.996 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here