Arthrobacter ulcerisalmonis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3300 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P5XBW6|A0A3P5XBW6_9MICC Uncharacterized protein OS=Arthrobacter ulcerisalmonis OX=2483813 GN=PSET11_02998 PE=4 SV=1
MM1 pKa = 7.26 SRR3 pKa = 11.84 ALIIVDD9 pKa = 3.67 VQNDD13 pKa = 3.62 FCEE16 pKa = 5.09 GGTLPVAGGSDD27 pKa = 3.36 LAGAISEE34 pKa = 4.56 YY35 pKa = 11.09 VDD37 pKa = 3.1 AHH39 pKa = 6.31 QGEE42 pKa = 4.65 FDD44 pKa = 4.08 HH45 pKa = 7.36 VVATQDD51 pKa = 3.0 WHH53 pKa = 6.62 VDD55 pKa = 3.43 PGGHH59 pKa = 6.56 FSAEE63 pKa = 3.98 PDD65 pKa = 4.67 FKK67 pKa = 11.33 DD68 pKa = 3.07 SWPPHH73 pKa = 5.67 CVAGTSGAEE82 pKa = 3.71 LHH84 pKa = 7.04 ADD86 pKa = 3.68 LDD88 pKa = 4.03 TEE90 pKa = 4.33 YY91 pKa = 10.72 IEE93 pKa = 5.29 AYY95 pKa = 9.2 FRR97 pKa = 11.84 KK98 pKa = 9.62 GQFSAAYY105 pKa = 9.7 SGFEE109 pKa = 3.89 GLLAPEE115 pKa = 4.76 DD116 pKa = 3.76 AVPTGEE122 pKa = 4.19 RR123 pKa = 11.84 QPGSMPGTEE132 pKa = 4.13 SQVDD136 pKa = 4.21 LDD138 pKa = 5.14 AVGLDD143 pKa = 3.15 EE144 pKa = 4.74 WLQEE148 pKa = 3.92 NDD150 pKa = 2.82 VDD152 pKa = 4.32 DD153 pKa = 4.21 VVIVGLAADD162 pKa = 3.71 YY163 pKa = 10.5 CVRR166 pKa = 11.84 ATALDD171 pKa = 3.88 AVQAGYY177 pKa = 10.92 GVTVVEE183 pKa = 4.84 ALTAGIGDD191 pKa = 4.22 DD192 pKa = 3.89 LSDD195 pKa = 3.47 VFAEE199 pKa = 4.28 LEE201 pKa = 4.1 LGGVEE206 pKa = 4.61 LAA208 pKa = 4.78
Molecular weight: 21.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.821
Patrickios 0.807
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A3P5WX77|A0A3P5WX77_9MICC Thymidylate kinase OS=Arthrobacter ulcerisalmonis OX=2483813 GN=tmk PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.65 KK12 pKa = 10.17 IPGAQIVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 8.65 LNPRR34 pKa = 11.84 MKK36 pKa = 10.4 ARR38 pKa = 11.84 QGG40 pKa = 3.24
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.574
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.31
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3300
0
3300
1050411
29
2089
318.3
33.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.459 ± 0.063
0.585 ± 0.009
5.615 ± 0.039
5.325 ± 0.038
3.202 ± 0.026
8.889 ± 0.034
2.045 ± 0.022
4.421 ± 0.032
2.759 ± 0.031
10.335 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.958 ± 0.018
2.506 ± 0.025
5.394 ± 0.029
3.313 ± 0.023
6.275 ± 0.041
5.969 ± 0.033
6.089 ± 0.035
8.47 ± 0.042
1.392 ± 0.017
2.0 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here