Nonomuraea sp. WAC 01424

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea; unclassified Nonomuraea

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429AA20|A0A429AA20_9ACTN TetR family transcriptional regulator OS=Nonomuraea sp. WAC 01424 OX=2203200 GN=DMB42_47295 PE=4 SV=1
MM1 pKa = 7.48NSRR4 pKa = 11.84TARR7 pKa = 11.84VLGLGAVILAAAATMTLPAQADD29 pKa = 3.8DD30 pKa = 3.52DD31 pKa = 4.54HH32 pKa = 7.85GYY34 pKa = 10.88LDD36 pKa = 3.79EE37 pKa = 4.92ALEE40 pKa = 4.3YY41 pKa = 11.17VDD43 pKa = 5.59VPYY46 pKa = 9.9CTGYY50 pKa = 11.11LEE52 pKa = 5.79LYY54 pKa = 10.73DD55 pKa = 5.49RR56 pKa = 11.84DD57 pKa = 3.9LCASPSRR64 pKa = 11.84STSSDD69 pKa = 2.91EE70 pKa = 5.27RR71 pKa = 11.84GDD73 pKa = 3.83LSDD76 pKa = 3.9AFEE79 pKa = 4.46GASPYY84 pKa = 11.12ARR86 pKa = 11.84GDD88 pKa = 3.64LSSDD92 pKa = 3.19APGDD96 pKa = 3.4ITLSDD101 pKa = 3.82EE102 pKa = 4.44VVQGNGG108 pKa = 2.94

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429AV91|A0A429AV91_9ACTN MFS transporter OS=Nonomuraea sp. WAC 01424 OX=2203200 GN=DMB42_31470 PE=4 SV=1
MM1 pKa = 7.42LRR3 pKa = 11.84LSNRR7 pKa = 11.84SGRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84TGAAAAPATPAAAQAGRR29 pKa = 11.84AGRR32 pKa = 11.84AGRR35 pKa = 11.84AGGRR39 pKa = 11.84AAGRR43 pKa = 11.84AAASRR48 pKa = 11.84SPAAGRR54 pKa = 11.84AGRR57 pKa = 11.84AGPAGTRR64 pKa = 11.84RR65 pKa = 11.84GARR68 pKa = 3.29

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10150

0

10150

3325445

25

6668

327.6

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.451 ± 0.037

0.779 ± 0.006

5.774 ± 0.017

5.547 ± 0.024

2.834 ± 0.014

9.48 ± 0.022

2.21 ± 0.012

3.334 ± 0.017

1.947 ± 0.017

10.858 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.01

1.719 ± 0.015

6.09 ± 0.021

2.625 ± 0.014

8.259 ± 0.026

4.898 ± 0.017

5.814 ± 0.021

8.785 ± 0.024

1.587 ± 0.009

2.13 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski