Amino acid dipepetide frequency for Nonomuraea sp. WAC 01424

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.404AlaAla: 21.404 ± 0.127	1.132AlaCys: 1.132 ± 0.021	7.952AlaAsp: 7.952 ± 0.06	8.448AlaGlu: 8.448 ± 0.058	3.779AlaPhe: 3.779 ± 0.037	13.91AlaGly: 13.91 ± 0.077	2.637AlaHis: 2.637 ± 0.029	3.952AlaIle: 3.952 ± 0.037	2.583AlaLys: 2.583 ± 0.037	14.524AlaLeu: 14.524 ± 0.102	2.784AlaMet: 2.784 ± 0.029	1.906AlaAsn: 1.906 ± 0.027	6.287AlaPro: 6.287 ± 0.052	3.418AlaGln: 3.418 ± 0.036	10.584AlaArg: 10.584 ± 0.067	5.409AlaSer: 5.409 ± 0.047	6.968AlaThr: 6.968 ± 0.051	12.049AlaVal: 12.049 ± 0.075	1.94AlaTrp: 1.94 ± 0.026	2.84AlaTyr: 2.84 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.047CysAla: 1.047 ± 0.019	0.096CysCys: 0.096 ± 0.006	0.487CysAsp: 0.487 ± 0.014	0.407CysGlu: 0.407 ± 0.012	0.22CysPhe: 0.22 ± 0.009	0.945CysGly: 0.945 ± 0.02	0.229CysHis: 0.229 ± 0.009	0.117CysIle: 0.117 ± 0.006	0.107CysLys: 0.107 ± 0.006	0.788CysLeu: 0.788 ± 0.015	0.13CysMet: 0.13 ± 0.006	0.117CysAsn: 0.117 ± 0.006	0.501CysPro: 0.501 ± 0.014	0.177CysGln: 0.177 ± 0.007	0.597CysArg: 0.597 ± 0.014	0.41CysSer: 0.41 ± 0.012	0.426CysThr: 0.426 ± 0.012	0.689CysVal: 0.689 ± 0.014	0.125CysTrp: 0.125 ± 0.007	0.178CysTyr: 0.178 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	6.734AspAla: 6.734 ± 0.048	0.379AspCys: 0.379 ± 0.01	3.606AspAsp: 3.606 ± 0.039	3.59AspGlu: 3.59 ± 0.04	1.538AspPhe: 1.538 ± 0.024	6.088AspGly: 6.088 ± 0.052	1.385AspHis: 1.385 ± 0.022	1.647AspIle: 1.647 ± 0.024	1.028AspLys: 1.028 ± 0.019	6.969AspLeu: 6.969 ± 0.055	0.805AspMet: 0.805 ± 0.014	0.932AspAsn: 0.932 ± 0.017	4.475AspPro: 4.475 ± 0.039	1.611AspGln: 1.611 ± 0.022	4.905AspArg: 4.905 ± 0.046	2.143AspSer: 2.143 ± 0.026	2.634AspThr: 2.634 ± 0.031	5.073AspVal: 5.073 ± 0.045	0.978AspTrp: 0.978 ± 0.018	1.215AspTyr: 1.215 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.92GluAla: 6.92 ± 0.062	0.354GluCys: 0.354 ± 0.011	2.444GluAsp: 2.444 ± 0.032	3.458GluGlu: 3.458 ± 0.043	1.42GluPhe: 1.42 ± 0.021	4.061GluGly: 4.061 ± 0.041	1.579GluHis: 1.579 ± 0.024	2.359GluIle: 2.359 ± 0.03	1.043GluLys: 1.043 ± 0.024	7.072GluLeu: 7.072 ± 0.059	0.859GluMet: 0.859 ± 0.017	0.865GluAsn: 0.865 ± 0.017	3.455GluPro: 3.455 ± 0.041	2.088GluGln: 2.088 ± 0.027	5.749GluArg: 5.749 ± 0.052	2.604GluSer: 2.604 ± 0.028	2.664GluThr: 2.664 ± 0.029	4.666GluVal: 4.666 ± 0.047	0.815GluTrp: 0.815 ± 0.015	0.997GluTyr: 0.997 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.881PheAla: 3.881 ± 0.038	0.27PheCys: 0.27 ± 0.01	2.034PheAsp: 2.034 ± 0.025	1.461PheGlu: 1.461 ± 0.024	0.903PhePhe: 0.903 ± 0.018	3.072PheGly: 3.072 ± 0.03	0.619PheHis: 0.619 ± 0.014	0.719PheIle: 0.719 ± 0.018	0.505PheLys: 0.505 ± 0.014	2.734PheLeu: 2.734 ± 0.031	0.469PheMet: 0.469 ± 0.012	0.567PheAsn: 0.567 ± 0.013	1.466PhePro: 1.466 ± 0.02	0.694PheGln: 0.694 ± 0.012	1.882PheArg: 1.882 ± 0.024	1.495PheSer: 1.495 ± 0.025	2.053PheThr: 2.053 ± 0.026	2.408PheVal: 2.408 ± 0.028	0.479PheTrp: 0.479 ± 0.012	0.628PheTyr: 0.628 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	10.471GlyAla: 10.471 ± 0.063	0.827GlyCys: 0.827 ± 0.017	5.296GlyAsp: 5.296 ± 0.045	5.26GlyGlu: 5.26 ± 0.044	2.952GlyPhe: 2.952 ± 0.035	9.0GlyGly: 9.0 ± 0.074	2.358GlyHis: 2.358 ± 0.029	3.155GlyIle: 3.155 ± 0.033	2.246GlyLys: 2.246 ± 0.036	10.36GlyLeu: 10.36 ± 0.062	2.113GlyMet: 2.113 ± 0.023	1.699GlyAsn: 1.699 ± 0.025	5.216GlyPro: 5.216 ± 0.042	2.762GlyGln: 2.762 ± 0.036	8.235GlyArg: 8.235 ± 0.065	4.804GlySer: 4.804 ± 0.047	5.704GlyThr: 5.704 ± 0.05	8.235GlyVal: 8.235 ± 0.059	1.776GlyTrp: 1.776 ± 0.024	2.333GlyTyr: 2.333 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	2.659HisAla: 2.659 ± 0.032	0.2HisCys: 0.2 ± 0.008	1.455HisAsp: 1.455 ± 0.021	1.234HisGlu: 1.234 ± 0.021	0.605HisPhe: 0.605 ± 0.013	2.21HisGly: 2.21 ± 0.026	0.635HisHis: 0.635 ± 0.017	0.619HisIle: 0.619 ± 0.014	0.31HisLys: 0.31 ± 0.009	2.481HisLeu: 2.481 ± 0.03	0.337HisMet: 0.337 ± 0.011	0.376HisAsn: 0.376 ± 0.011	1.7HisPro: 1.7 ± 0.022	0.601HisGln: 0.601 ± 0.015	1.913HisArg: 1.913 ± 0.028	0.878HisSer: 0.878 ± 0.019	1.144HisThr: 1.144 ± 0.019	1.905HisVal: 1.905 ± 0.026	0.352HisTrp: 0.352 ± 0.01	0.48HisTyr: 0.48 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.864IleAla: 4.864 ± 0.042	0.292IleCys: 0.292 ± 0.011	2.335IleAsp: 2.335 ± 0.027	2.092IleGlu: 2.092 ± 0.031	0.835IlePhe: 0.835 ± 0.016	3.584IleGly: 3.584 ± 0.039	0.595IleHis: 0.595 ± 0.013	0.989IleIle: 0.989 ± 0.02	0.746IleLys: 0.746 ± 0.015	2.571IleLeu: 2.571 ± 0.028	0.6IleMet: 0.6 ± 0.015	0.681IleAsn: 0.681 ± 0.016	1.773IlePro: 1.773 ± 0.026	0.713IleGln: 0.713 ± 0.018	2.364IleArg: 2.364 ± 0.029	1.875IleSer: 1.875 ± 0.028	2.293IleThr: 2.293 ± 0.027	3.07IleVal: 3.07 ± 0.035	0.459IleTrp: 0.459 ± 0.014	0.611IleTyr: 0.611 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.564LysAla: 2.564 ± 0.033	0.111LysCys: 0.111 ± 0.006	1.124LysAsp: 1.124 ± 0.022	1.109LysGlu: 1.109 ± 0.022	0.417LysPhe: 0.417 ± 0.012	1.796LysGly: 1.796 ± 0.031	0.39LysHis: 0.39 ± 0.012	0.934LysIle: 0.934 ± 0.019	0.607LysLys: 0.607 ± 0.02	1.947LysLeu: 1.947 ± 0.03	0.352LysMet: 0.352 ± 0.012	0.427LysAsn: 0.427 ± 0.014	1.241LysPro: 1.241 ± 0.023	0.584LysGln: 0.584 ± 0.016	1.262LysArg: 1.262 ± 0.023	1.011LysSer: 1.011 ± 0.019	1.089LysThr: 1.089 ± 0.021	1.848LysVal: 1.848 ± 0.031	0.267LysTrp: 0.267 ± 0.01	0.392LysTyr: 0.392 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	16.596LeuAla: 16.596 ± 0.105	0.793LeuCys: 0.793 ± 0.016	6.963LeuAsp: 6.963 ± 0.054	4.798LeuGlu: 4.798 ± 0.042	2.849LeuPhe: 2.849 ± 0.032	9.674LeuGly: 9.674 ± 0.066	2.141LeuHis: 2.141 ± 0.03	3.743LeuIle: 3.743 ± 0.042	1.903LeuLys: 1.903 ± 0.027	11.762LeuLeu: 11.762 ± 0.09	1.784LeuMet: 1.784 ± 0.025	1.798LeuAsn: 1.798 ± 0.024	6.783LeuPro: 6.783 ± 0.055	2.095LeuGln: 2.095 ± 0.025	9.269LeuArg: 9.269 ± 0.065	5.761LeuSer: 5.761 ± 0.04	7.119LeuThr: 7.119 ± 0.049	9.443LeuVal: 9.443 ± 0.069	1.358LeuTrp: 1.358 ± 0.023	1.947LeuTyr: 1.947 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.433MetAla: 2.433 ± 0.026	0.131MetCys: 0.131 ± 0.006	0.922MetAsp: 0.922 ± 0.016	0.799MetGlu: 0.799 ± 0.016	0.524MetPhe: 0.524 ± 0.014	1.39MetGly: 1.39 ± 0.023	0.34MetHis: 0.34 ± 0.011	0.88MetIle: 0.88 ± 0.016	0.427MetLys: 0.427 ± 0.013	2.008MetLeu: 2.008 ± 0.026	0.349MetMet: 0.349 ± 0.01	0.468MetAsn: 0.468 ± 0.013	1.15MetPro: 1.15 ± 0.019	0.404MetGln: 0.404 ± 0.011	1.709MetArg: 1.709 ± 0.024	1.351MetSer: 1.351 ± 0.016	1.551MetThr: 1.551 ± 0.022	1.376MetVal: 1.376 ± 0.022	0.253MetTrp: 0.253 ± 0.008	0.328MetTyr: 0.328 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.093AsnAla: 2.093 ± 0.026	0.16AsnCys: 0.16 ± 0.007	0.903AsnAsp: 0.903 ± 0.019	0.801AsnGlu: 0.801 ± 0.016	0.466AsnPhe: 0.466 ± 0.014	1.841AsnGly: 1.841 ± 0.03	0.382AsnHis: 0.382 ± 0.014	0.578AsnIle: 0.578 ± 0.013	0.35AsnLys: 0.35 ± 0.011	1.795AsnLeu: 1.795 ± 0.024	0.285AsnMet: 0.285 ± 0.01	0.387AsnAsn: 0.387 ± 0.014	1.352AsnPro: 1.352 ± 0.025	0.489AsnGln: 0.489 ± 0.013	1.292AsnArg: 1.292 ± 0.02	0.828AsnSer: 0.828 ± 0.016	1.012AsnThr: 1.012 ± 0.023	1.502AsnVal: 1.502 ± 0.023	0.281AsnTrp: 0.281 ± 0.009	0.393AsnTyr: 0.393 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.629ProAla: 8.629 ± 0.055	0.358ProCys: 0.358 ± 0.01	4.333ProAsp: 4.333 ± 0.04	4.128ProGlu: 4.128 ± 0.036	1.634ProPhe: 1.634 ± 0.025	6.978ProGly: 6.978 ± 0.056	1.229ProHis: 1.229 ± 0.022	1.618ProIle: 1.618 ± 0.024	1.123ProLys: 1.123 ± 0.023	5.473ProLeu: 5.473 ± 0.047	1.165ProMet: 1.165 ± 0.018	0.867ProAsn: 0.867 ± 0.019	3.908ProPro: 3.908 ± 0.052	1.509ProGln: 1.509 ± 0.025	3.956ProArg: 3.956 ± 0.036	3.154ProSer: 3.154 ± 0.033	2.89ProThr: 2.89 ± 0.037	5.443ProVal: 5.443 ± 0.044	0.967ProTrp: 0.967 ± 0.018	1.535ProTyr: 1.535 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	3.93GlnAla: 3.93 ± 0.038	0.15GlnCys: 0.15 ± 0.007	1.307GlnAsp: 1.307 ± 0.025	1.389GlnGlu: 1.389 ± 0.021	0.641GlnPhe: 0.641 ± 0.016	2.205GlnGly: 2.205 ± 0.025	0.567GlnHis: 0.567 ± 0.014	1.125GlnIle: 1.125 ± 0.017	0.498GlnLys: 0.498 ± 0.014	2.718GlnLeu: 2.718 ± 0.032	0.454GlnMet: 0.454 ± 0.013	0.467GlnAsn: 0.467 ± 0.013	1.62GlnPro: 1.62 ± 0.029	0.975GlnGln: 0.975 ± 0.027	2.185GlnArg: 2.185 ± 0.029	1.149GlnSer: 1.149 ± 0.019	1.258GlnThr: 1.258 ± 0.023	2.653GlnVal: 2.653 ± 0.028	0.434GlnTrp: 0.434 ± 0.011	0.522GlnTyr: 0.522 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	10.027ArgAla: 10.027 ± 0.077	0.605ArgCys: 0.605 ± 0.014	4.439ArgAsp: 4.439 ± 0.041	4.746ArgGlu: 4.746 ± 0.043	2.47ArgPhe: 2.47 ± 0.029	5.799ArgGly: 5.799 ± 0.047	2.25ArgHis: 2.25 ± 0.028	3.015ArgIle: 3.015 ± 0.034	1.514ArgLys: 1.514 ± 0.027	10.037ArgLeu: 10.037 ± 0.077	1.895ArgMet: 1.895 ± 0.023	1.34ArgAsn: 1.34 ± 0.024	5.262ArgPro: 5.262 ± 0.051	2.459ArgGln: 2.459 ± 0.03	8.202ArgArg: 8.202 ± 0.068	3.835ArgSer: 3.835 ± 0.035	4.893ArgThr: 4.893 ± 0.042	6.5ArgVal: 6.5 ± 0.047	1.424ArgTrp: 1.424 ± 0.021	1.88ArgTyr: 1.88 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.311SerAla: 6.311 ± 0.047	0.407SerCys: 0.407 ± 0.012	2.419SerAsp: 2.419 ± 0.03	2.245SerGlu: 2.245 ± 0.026	1.57SerPhe: 1.57 ± 0.023	5.821SerGly: 5.821 ± 0.057	0.924SerHis: 0.924 ± 0.016	1.614SerIle: 1.614 ± 0.024	0.961SerLys: 0.961 ± 0.023	4.899SerLeu: 4.899 ± 0.044	1.187SerMet: 1.187 ± 0.019	0.8SerAsn: 0.8 ± 0.016	3.192SerPro: 3.192 ± 0.031	1.173SerGln: 1.173 ± 0.02	3.71SerArg: 3.71 ± 0.037	2.677SerSer: 2.677 ± 0.041	2.749SerThr: 2.749 ± 0.034	4.068SerVal: 4.068 ± 0.041	1.008SerTrp: 1.008 ± 0.019	1.242SerTyr: 1.242 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	7.782ThrAla: 7.782 ± 0.056	0.458ThrCys: 0.458 ± 0.013	2.917ThrAsp: 2.917 ± 0.035	2.658ThrGlu: 2.658 ± 0.028	1.771ThrPhe: 1.771 ± 0.024	6.461ThrGly: 6.461 ± 0.051	1.089ThrHis: 1.089 ± 0.018	1.988ThrIle: 1.988 ± 0.028	1.062ThrLys: 1.062 ± 0.022	6.034ThrLeu: 6.034 ± 0.048	1.122ThrMet: 1.122 ± 0.018	0.921ThrAsn: 0.921 ± 0.02	4.021ThrPro: 4.021 ± 0.04	1.303ThrGln: 1.303 ± 0.026	4.011ThrArg: 4.011 ± 0.032	3.046ThrSer: 3.046 ± 0.036	3.487ThrThr: 3.487 ± 0.045	5.651ThrVal: 5.651 ± 0.047	1.029ThrTrp: 1.029 ± 0.017	1.331ThrTyr: 1.331 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	11.721ValAla: 11.721 ± 0.076	0.743ValCys: 0.743 ± 0.018	4.466ValAsp: 4.466 ± 0.037	4.922ValGlu: 4.922 ± 0.043	2.548ValPhe: 2.548 ± 0.031	6.605ValGly: 6.605 ± 0.052	1.878ValHis: 1.878 ± 0.025	3.213ValIle: 3.213 ± 0.034	1.76ValLys: 1.76 ± 0.031	10.118ValLeu: 10.118 ± 0.071	1.502ValMet: 1.502 ± 0.023	1.752ValAsn: 1.752 ± 0.026	5.503ValPro: 5.503 ± 0.046	1.961ValGln: 1.961 ± 0.028	7.403ValArg: 7.403 ± 0.054	4.604ValSer: 4.604 ± 0.042	5.956ValThr: 5.956 ± 0.052	8.37ValVal: 8.37 ± 0.066	1.194ValTrp: 1.194 ± 0.024	1.626ValTyr: 1.626 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.707TrpAla: 1.707 ± 0.026	0.155TrpCys: 0.155 ± 0.007	0.831TrpAsp: 0.831 ± 0.017	0.72TrpGlu: 0.72 ± 0.014	0.528TrpPhe: 0.528 ± 0.014	1.076TrpGly: 1.076 ± 0.018	0.43TrpHis: 0.43 ± 0.012	0.6TrpIle: 0.6 ± 0.013	0.336TrpLys: 0.336 ± 0.01	1.946TrpLeu: 1.946 ± 0.027	0.325TrpMet: 0.325 ± 0.009	0.413TrpAsn: 0.413 ± 0.011	0.902TrpPro: 0.902 ± 0.016	0.603TrpGln: 0.603 ± 0.014	1.497TrpArg: 1.497 ± 0.023	0.983TrpSer: 0.983 ± 0.021	1.059TrpThr: 1.059 ± 0.02	1.038TrpVal: 1.038 ± 0.02	0.37TrpTrp: 0.37 ± 0.011	0.355TrpTyr: 0.355 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.732TyrAla: 2.732 ± 0.032	0.174TyrCys: 0.174 ± 0.007	1.504TyrAsp: 1.504 ± 0.029	1.209TyrGlu: 1.209 ± 0.018	0.669TyrPhe: 0.669 ± 0.014	2.286TyrGly: 2.286 ± 0.033	0.437TyrHis: 0.437 ± 0.01	0.478TyrIle: 0.478 ± 0.013	0.362TyrLys: 0.362 ± 0.012	2.342TyrLeu: 2.342 ± 0.027	0.276TyrMet: 0.276 ± 0.009	0.408TyrAsn: 0.408 ± 0.012	1.089TyrPro: 1.089 ± 0.017	0.628TyrGln: 0.628 ± 0.015	1.864TyrArg: 1.864 ± 0.024	0.963TyrSer: 0.963 ± 0.019	1.191TyrThr: 1.191 ± 0.023	1.859TyrVal: 1.859 ± 0.027	0.363TyrTrp: 0.363 ± 0.01	0.466TyrTyr: 0.466 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10150 proteins (3325445 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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