Propionibacteriaceae bacterium
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2125 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q5YQ35|A0A4Q5YQ35_9ACTN Helix-turn-helix domain-containing protein OS=Propionibacteriaceae bacterium OX=2021380 GN=EOP01_10855 PE=4 SV=1
MM1 pKa = 7.23 SRR3 pKa = 11.84 IPEE6 pKa = 4.42 PEE8 pKa = 4.02 TTPDD12 pKa = 3.15 GPAYY16 pKa = 9.95 EE17 pKa = 4.68 GRR19 pKa = 11.84 LLDD22 pKa = 3.95 RR23 pKa = 11.84 ADD25 pKa = 4.13 EE26 pKa = 4.22 EE27 pKa = 5.11 VVDD30 pKa = 3.84 QGVSFDD36 pKa = 3.53 VRR38 pKa = 11.84 TLLSRR43 pKa = 11.84 RR44 pKa = 11.84 TVLGLLGIGAGSLALAACTTASGASDD70 pKa = 3.56 GTVSGAATSGSGAATTTAGEE90 pKa = 4.52 IPDD93 pKa = 4.12 EE94 pKa = 4.34 TAGPYY99 pKa = 10.2 PGDD102 pKa = 3.58 GSNGVDD108 pKa = 3.29 VLEE111 pKa = 3.99 QSGIVRR117 pKa = 11.84 SDD119 pKa = 2.68 IRR121 pKa = 11.84 SSIGGGATAEE131 pKa = 4.21 GVPMALQLTVLDD143 pKa = 4.55 MANDD147 pKa = 3.95 DD148 pKa = 4.18 APFEE152 pKa = 4.32 GVAVYY157 pKa = 9.96 VWHH160 pKa = 7.4 CDD162 pKa = 3.16 AAGGYY167 pKa = 10.35 SMYY170 pKa = 10.88 SEE172 pKa = 4.94 GIEE175 pKa = 4.15 DD176 pKa = 3.44 EE177 pKa = 4.52 TYY179 pKa = 10.93 LRR181 pKa = 11.84 GVQVADD187 pKa = 3.27 ATGTVSYY194 pKa = 10.43 TSIFPACYY202 pKa = 8.84 TT203 pKa = 3.7
Molecular weight: 20.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.605
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.935
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A4Q5Z8M0|A0A4Q5Z8M0_9ACTN Pyridoxamine 5'-phosphate oxidase OS=Propionibacteriaceae bacterium OX=2021380 GN=EOP01_02285 PE=4 SV=1
AA1 pKa = 7.88 CPRR4 pKa = 11.84 AAAPSSCAPDD14 pKa = 3.5 RR15 pKa = 11.84 ARR17 pKa = 11.84 TPSHH21 pKa = 7.04 PSSGPPPSRR30 pKa = 11.84 PRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 ARR36 pKa = 11.84 RR37 pKa = 11.84 GSRR40 pKa = 11.84 GHH42 pKa = 6.06 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 GRR47 pKa = 11.84 RR48 pKa = 11.84 SSRR51 pKa = 11.84 GPGRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 GRR59 pKa = 11.84 PPASGATPAPPSSRR73 pKa = 11.84 PSGTPAARR81 pKa = 11.84 RR82 pKa = 11.84 TAGSPSPAAAGRR94 pKa = 11.84 GTGPAPRR101 pKa = 11.84 PAGGAAGHH109 pKa = 6.6 RR110 pKa = 11.84 GRR112 pKa = 11.84 WFRR115 pKa = 11.84 ARR117 pKa = 11.84 RR118 pKa = 11.84 PRR120 pKa = 11.84 GRR122 pKa = 11.84 TRR124 pKa = 11.84 RR125 pKa = 3.63
Molecular weight: 13.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.369
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.091
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.175
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2125
0
2125
451348
23
809
212.4
22.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.493 ± 0.08
0.715 ± 0.019
6.325 ± 0.049
5.348 ± 0.065
2.911 ± 0.03
9.159 ± 0.053
2.17 ± 0.031
3.496 ± 0.048
2.281 ± 0.046
10.079 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.776 ± 0.022
2.012 ± 0.031
5.478 ± 0.044
3.074 ± 0.035
7.559 ± 0.062
5.526 ± 0.047
6.37 ± 0.043
9.539 ± 0.054
1.454 ± 0.023
2.234 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here