Propionibacteriaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; unclassified Propionibacteriaceae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Q5YQ35|A0A4Q5YQ35_9ACTN Helix-turn-helix domain-containing protein OS=Propionibacteriaceae bacterium OX=2021380 GN=EOP01_10855 PE=4 SV=1
MM1 pKa = 7.23SRR3 pKa = 11.84IPEE6 pKa = 4.42PEE8 pKa = 4.02TTPDD12 pKa = 3.15GPAYY16 pKa = 9.95EE17 pKa = 4.68GRR19 pKa = 11.84LLDD22 pKa = 3.95RR23 pKa = 11.84ADD25 pKa = 4.13EE26 pKa = 4.22EE27 pKa = 5.11VVDD30 pKa = 3.84QGVSFDD36 pKa = 3.53VRR38 pKa = 11.84TLLSRR43 pKa = 11.84RR44 pKa = 11.84TVLGLLGIGAGSLALAACTTASGASDD70 pKa = 3.56GTVSGAATSGSGAATTTAGEE90 pKa = 4.52IPDD93 pKa = 4.12EE94 pKa = 4.34TAGPYY99 pKa = 10.2PGDD102 pKa = 3.58GSNGVDD108 pKa = 3.29VLEE111 pKa = 3.99QSGIVRR117 pKa = 11.84SDD119 pKa = 2.68IRR121 pKa = 11.84SSIGGGATAEE131 pKa = 4.21GVPMALQLTVLDD143 pKa = 4.55MANDD147 pKa = 3.95DD148 pKa = 4.18APFEE152 pKa = 4.32GVAVYY157 pKa = 9.96VWHH160 pKa = 7.4CDD162 pKa = 3.16AAGGYY167 pKa = 10.35SMYY170 pKa = 10.88SEE172 pKa = 4.94GIEE175 pKa = 4.15DD176 pKa = 3.44EE177 pKa = 4.52TYY179 pKa = 10.93LRR181 pKa = 11.84GVQVADD187 pKa = 3.27ATGTVSYY194 pKa = 10.43TSIFPACYY202 pKa = 8.84TT203 pKa = 3.7

Molecular weight:
20.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q5Z8M0|A0A4Q5Z8M0_9ACTN Pyridoxamine 5'-phosphate oxidase OS=Propionibacteriaceae bacterium OX=2021380 GN=EOP01_02285 PE=4 SV=1
AA1 pKa = 7.88CPRR4 pKa = 11.84AAAPSSCAPDD14 pKa = 3.5RR15 pKa = 11.84ARR17 pKa = 11.84TPSHH21 pKa = 7.04PSSGPPPSRR30 pKa = 11.84PRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84ARR36 pKa = 11.84RR37 pKa = 11.84GSRR40 pKa = 11.84GHH42 pKa = 6.06RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84GRR47 pKa = 11.84RR48 pKa = 11.84SSRR51 pKa = 11.84GPGRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84GRR59 pKa = 11.84PPASGATPAPPSSRR73 pKa = 11.84PSGTPAARR81 pKa = 11.84RR82 pKa = 11.84TAGSPSPAAAGRR94 pKa = 11.84GTGPAPRR101 pKa = 11.84PAGGAAGHH109 pKa = 6.6RR110 pKa = 11.84GRR112 pKa = 11.84WFRR115 pKa = 11.84ARR117 pKa = 11.84RR118 pKa = 11.84PRR120 pKa = 11.84GRR122 pKa = 11.84TRR124 pKa = 11.84RR125 pKa = 3.63

Molecular weight:
13.06 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2125

0

2125

451348

23

809

212.4

22.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.493 ± 0.08

0.715 ± 0.019

6.325 ± 0.049

5.348 ± 0.065

2.911 ± 0.03

9.159 ± 0.053

2.17 ± 0.031

3.496 ± 0.048

2.281 ± 0.046

10.079 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.022

2.012 ± 0.031

5.478 ± 0.044

3.074 ± 0.035

7.559 ± 0.062

5.526 ± 0.047

6.37 ± 0.043

9.539 ± 0.054

1.454 ± 0.023

2.234 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski