Streptococcus phage Javan526

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BE48|A0A4D6BE48_9CAUD Uncharacterized protein OS=Streptococcus phage Javan526 OX=2548239 GN=Javan526_0009 PE=4 SV=1
MM1 pKa = 7.82LIYY4 pKa = 10.15PATFTQDD11 pKa = 2.07TDD13 pKa = 4.11YY14 pKa = 11.77IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.63AITQGEE30 pKa = 4.54DD31 pKa = 3.17FQEE34 pKa = 4.6AYY36 pKa = 10.36EE37 pKa = 4.17MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.25DD49 pKa = 3.43YY50 pKa = 10.49TDD52 pKa = 3.81YY53 pKa = 11.16PKK55 pKa = 11.16ASAVSDD61 pKa = 3.95LKK63 pKa = 10.93EE64 pKa = 3.92QYY66 pKa = 9.46PDD68 pKa = 3.46SDD70 pKa = 3.42IALIGIDD77 pKa = 3.16MVAYY81 pKa = 7.98MKK83 pKa = 10.29KK84 pKa = 8.58YY85 pKa = 9.49HH86 pKa = 5.88SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.28WLNDD102 pKa = 3.5AAEE105 pKa = 4.41DD106 pKa = 3.75KK107 pKa = 10.95NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BAD0|A0A4D6BAD0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan526 OX=2548239 GN=Javan526_0005 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.4RR9 pKa = 11.84KK10 pKa = 8.96LEE12 pKa = 3.96TAIVLLIKK20 pKa = 10.27EE21 pKa = 4.31VAEE24 pKa = 3.97LRR26 pKa = 11.84SIVSANAKK34 pKa = 8.06ATNNEE39 pKa = 3.65LAAVKK44 pKa = 10.33SAILDD49 pKa = 3.45NQVAIKK55 pKa = 10.63SIGDD59 pKa = 3.5EE60 pKa = 4.1VGHH63 pKa = 6.84IKK65 pKa = 10.4QNYY68 pKa = 6.67KK69 pKa = 10.51RR70 pKa = 11.84KK71 pKa = 8.22WRR73 pKa = 11.84KK74 pKa = 8.88

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12293

41

1307

195.1

22.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.931 ± 0.677

0.618 ± 0.099

6.646 ± 0.286

7.281 ± 0.303

3.864 ± 0.277

6.321 ± 0.267

1.35 ± 0.132

7.159 ± 0.332

9.013 ± 0.347

8.672 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.157

5.849 ± 0.254

2.815 ± 0.204

3.88 ± 0.219

3.978 ± 0.204

6.15 ± 0.299

6.109 ± 0.23

5.865 ± 0.238

1.139 ± 0.135

3.823 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski