Dysgonomonas capnocytophagoides
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3764 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y8KU10|A0A4Y8KU10_9BACT Uncharacterized protein OS=Dysgonomonas capnocytophagoides OX=45254 GN=E2605_18355 PE=4 SV=1
MM1 pKa = 7.55 IKK3 pKa = 10.06 QYY5 pKa = 10.91 NYY7 pKa = 10.0 EE8 pKa = 4.07 YY9 pKa = 10.69 GFAEE13 pKa = 4.17 ATVSFQVDD21 pKa = 3.23 TDD23 pKa = 3.69 VFTAEE28 pKa = 4.66 LANEE32 pKa = 4.07 TLTFFTWDD40 pKa = 3.36 YY41 pKa = 11.69 DD42 pKa = 3.67 KK43 pKa = 11.58 DD44 pKa = 3.83 ADD46 pKa = 4.63 PVDD49 pKa = 4.16 EE50 pKa = 4.3 VMKK53 pKa = 10.21 KK54 pKa = 10.31 YY55 pKa = 10.95 AMEE58 pKa = 5.15 AIKK61 pKa = 10.46 QATFNNYY68 pKa = 9.22 SEE70 pKa = 4.74 YY71 pKa = 11.06 GVISAFEE78 pKa = 4.55 DD79 pKa = 3.58 NEE81 pKa = 4.37 GFGRR85 pKa = 11.84 LDD87 pKa = 3.29 GSIGITLLEE96 pKa = 4.0 VTGYY100 pKa = 11.0 DD101 pKa = 3.37 FDD103 pKa = 4.42 EE104 pKa = 5.7 SEE106 pKa = 4.41 LTLEE110 pKa = 4.6 SEE112 pKa = 4.52
Molecular weight: 12.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.452
ProMoST 3.77
Dawson 3.63
Bjellqvist 3.834
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.363
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A4Y8L4D1|A0A4Y8L4D1_9BACT UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase OS=Dysgonomonas capnocytophagoides OX=45254 GN=murG PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 10.03 RR12 pKa = 11.84 INKK15 pKa = 9.29 HH16 pKa = 3.82 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MATANGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.27 GRR39 pKa = 11.84 AKK41 pKa = 10.71 LSVSDD46 pKa = 4.22 EE47 pKa = 4.0 AAKK50 pKa = 10.7
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 10.687
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.164
Grimsley 12.471
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.901
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3764
0
3764
1361703
25
2491
361.8
40.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.308 ± 0.039
0.943 ± 0.014
5.916 ± 0.025
6.092 ± 0.037
4.727 ± 0.029
6.54 ± 0.037
1.611 ± 0.016
7.96 ± 0.036
7.438 ± 0.032
8.924 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.017
5.839 ± 0.035
3.447 ± 0.02
3.354 ± 0.023
3.903 ± 0.026
7.06 ± 0.032
5.668 ± 0.028
6.047 ± 0.031
1.22 ± 0.015
4.674 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here