Streptomyces sp. AgN23

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F7VQ29|A0A6F7VQ29_9ACTN NADH-quinone oxidoreductase subunit A OS=Streptomyces sp. AgN23 OX=1188315 GN=nuoA PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84MYY4 pKa = 10.32RR5 pKa = 11.84TMAAAATLALALGGAALAAPAAQAAATTSGSLVHH39 pKa = 7.09EE40 pKa = 5.54DD41 pKa = 3.54GEE43 pKa = 4.49LWYY46 pKa = 10.53KK47 pKa = 10.45AAAGQKK53 pKa = 9.77NNLSVSEE60 pKa = 4.2QIVSRR65 pKa = 11.84GEE67 pKa = 3.71FEE69 pKa = 4.1EE70 pKa = 5.66YY71 pKa = 10.54YY72 pKa = 11.25VLTFRR77 pKa = 11.84DD78 pKa = 3.77NGDD81 pKa = 2.91ITIDD85 pKa = 3.91AEE87 pKa = 3.98AADD90 pKa = 4.52WDD92 pKa = 4.01EE93 pKa = 4.28CTYY96 pKa = 8.96PTAGDD101 pKa = 3.56HH102 pKa = 6.36TVAQCAVLIPQNSDD116 pKa = 3.21DD117 pKa = 3.95SDD119 pKa = 4.15NYY121 pKa = 11.38DD122 pKa = 3.0VDD124 pKa = 5.67LGDD127 pKa = 4.68GDD129 pKa = 4.54DD130 pKa = 4.37TIKK133 pKa = 11.07LDD135 pKa = 4.25ADD137 pKa = 3.43SSAYY141 pKa = 10.39AGIHH145 pKa = 5.97GGAGDD150 pKa = 5.29DD151 pKa = 3.73VLKK154 pKa = 11.28GNAAPMFYY162 pKa = 10.96GEE164 pKa = 5.66DD165 pKa = 3.57GDD167 pKa = 4.6DD168 pKa = 3.87QIDD171 pKa = 3.77GGGGVMGFGAYY182 pKa = 10.05GGDD185 pKa = 3.69GDD187 pKa = 5.06DD188 pKa = 4.57TITNCVQEE196 pKa = 4.3CHH198 pKa = 6.56GDD200 pKa = 3.57AGDD203 pKa = 3.75DD204 pKa = 4.08TITGGAEE211 pKa = 3.76DD212 pKa = 4.17NILRR216 pKa = 11.84GGTGKK221 pKa = 10.54DD222 pKa = 2.76ILRR225 pKa = 11.84GGKK228 pKa = 9.68GADD231 pKa = 4.08AIYY234 pKa = 10.67GDD236 pKa = 4.03QDD238 pKa = 4.34DD239 pKa = 4.09DD240 pKa = 3.86TLYY243 pKa = 11.47GEE245 pKa = 5.37DD246 pKa = 4.59GNDD249 pKa = 3.43TIYY252 pKa = 11.26GNSGNDD258 pKa = 3.47VLWGGRR264 pKa = 11.84GTDD267 pKa = 3.64TLSGGPGRR275 pKa = 11.84NEE277 pKa = 3.68VHH279 pKa = 6.65QDD281 pKa = 2.84

Molecular weight: 28.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F7W0M9|A0A6F7W0M9_9ACTN ABC transporter OS=Streptomyces sp. AgN23 OX=1188315 GN=AS97_37335 PE=4 SV=1
MM1 pKa = 7.38SPRR4 pKa = 11.84RR5 pKa = 11.84NRR7 pKa = 11.84PRR9 pKa = 11.84GGEE12 pKa = 3.88KK13 pKa = 10.23PVGHH17 pKa = 6.72EE18 pKa = 3.71SANRR22 pKa = 11.84YY23 pKa = 9.37GLDD26 pKa = 5.08RR27 pKa = 11.84MEE29 pKa = 4.01TWQGEE34 pKa = 4.39EE35 pKa = 3.8WVVRR39 pKa = 11.84QVGGGAAAKK48 pKa = 9.9HH49 pKa = 5.06YY50 pKa = 9.18RR51 pKa = 11.84CPGCDD56 pKa = 3.24QEE58 pKa = 4.75IPPGVAHH65 pKa = 5.96VVAWQQHH72 pKa = 4.58MGADD76 pKa = 5.12DD77 pKa = 3.31RR78 pKa = 11.84RR79 pKa = 11.84HH80 pKa = 3.84WHH82 pKa = 5.64KK83 pKa = 11.08ACWSARR89 pKa = 11.84DD90 pKa = 3.54RR91 pKa = 11.84RR92 pKa = 11.84SAKK95 pKa = 9.7LQRR98 pKa = 11.84SRR100 pKa = 11.84NAPRR104 pKa = 11.84YY105 pKa = 8.88

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5582

0

5582

1629245

33

2605

291.9

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.027 ± 0.043

0.796 ± 0.009

6.008 ± 0.027

5.903 ± 0.037

2.834 ± 0.018

9.459 ± 0.034

2.348 ± 0.017

3.487 ± 0.02

2.319 ± 0.029

10.231 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.897 ± 0.012

1.803 ± 0.017

5.95 ± 0.026

2.763 ± 0.018

7.996 ± 0.036

5.174 ± 0.027

6.053 ± 0.029

8.312 ± 0.032

1.496 ± 0.013

2.144 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski