Amino acid dipepetide frequency for Streptomyces sp. AgN23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.387AlaAla: 19.387 ± 0.163
0.987AlaCys: 0.987 ± 0.025
8.134AlaAsp: 8.134 ± 0.078
8.881AlaGlu: 8.881 ± 0.09
3.601AlaPhe: 3.601 ± 0.052
12.045AlaGly: 12.045 ± 0.091
2.829AlaHis: 2.829 ± 0.046
3.986AlaIle: 3.986 ± 0.056
3.043AlaLys: 3.043 ± 0.056
14.094AlaLeu: 14.094 ± 0.114
2.723AlaMet: 2.723 ± 0.035
1.962AlaAsn: 1.962 ± 0.037
6.487AlaPro: 6.487 ± 0.067
3.72AlaGln: 3.72 ± 0.052
9.599AlaArg: 9.599 ± 0.089
5.74AlaSer: 5.74 ± 0.066
7.031AlaThr: 7.031 ± 0.077
11.542AlaVal: 11.542 ± 0.106
1.765AlaTrp: 1.765 ± 0.033
2.714AlaTyr: 2.714 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.1CysAla: 1.1 ± 0.031
0.095CysCys: 0.095 ± 0.008
0.476CysAsp: 0.476 ± 0.018
0.435CysGlu: 0.435 ± 0.017
0.23CysPhe: 0.23 ± 0.012
0.951CysGly: 0.951 ± 0.024
0.198CysHis: 0.198 ± 0.011
0.158CysIle: 0.158 ± 0.011
0.123CysLys: 0.123 ± 0.01
0.7CysLeu: 0.7 ± 0.021
0.138CysMet: 0.138 ± 0.009
0.136CysAsn: 0.136 ± 0.011
0.486CysPro: 0.486 ± 0.02
0.182CysGln: 0.182 ± 0.011
0.612CysArg: 0.612 ± 0.019
0.459CysSer: 0.459 ± 0.018
0.488CysThr: 0.488 ± 0.02
0.709CysVal: 0.709 ± 0.019
0.129CysTrp: 0.129 ± 0.009
0.158CysTyr: 0.158 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.489AspAla: 7.489 ± 0.066
0.403AspCys: 0.403 ± 0.018
3.583AspAsp: 3.583 ± 0.057
3.947AspGlu: 3.947 ± 0.051
1.718AspPhe: 1.718 ± 0.034
6.402AspGly: 6.402 ± 0.071
1.58AspHis: 1.58 ± 0.03
2.123AspIle: 2.123 ± 0.044
1.283AspLys: 1.283 ± 0.036
6.103AspLeu: 6.103 ± 0.072
0.857AspMet: 0.857 ± 0.024
0.99AspAsn: 0.99 ± 0.023
4.521AspPro: 4.521 ± 0.051
1.717AspGln: 1.717 ± 0.034
4.927AspArg: 4.927 ± 0.061
2.573AspSer: 2.573 ± 0.044
3.26AspThr: 3.26 ± 0.046
4.419AspVal: 4.419 ± 0.055
0.971AspTrp: 0.971 ± 0.023
1.217AspTyr: 1.217 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.532GluAla: 7.532 ± 0.089
0.392GluCys: 0.392 ± 0.015
2.975GluAsp: 2.975 ± 0.049
3.921GluGlu: 3.921 ± 0.061
1.49GluPhe: 1.49 ± 0.027
4.597GluGly: 4.597 ± 0.061
1.544GluHis: 1.544 ± 0.033
2.386GluIle: 2.386 ± 0.043
1.577GluLys: 1.577 ± 0.034
6.975GluLeu: 6.975 ± 0.078
0.992GluMet: 0.992 ± 0.025
1.053GluAsn: 1.053 ± 0.03
3.461GluPro: 3.461 ± 0.049
2.112GluGln: 2.112 ± 0.035
5.837GluArg: 5.837 ± 0.078
2.646GluSer: 2.646 ± 0.043
2.966GluThr: 2.966 ± 0.039
4.656GluVal: 4.656 ± 0.059
0.779GluTrp: 0.779 ± 0.022
1.137GluTyr: 1.137 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
3.68PheAla: 3.68 ± 0.052
0.279PheCys: 0.279 ± 0.013
2.036PheAsp: 2.036 ± 0.041
1.499PheGlu: 1.499 ± 0.037
0.943PhePhe: 0.943 ± 0.03
3.187PheGly: 3.187 ± 0.049
0.654PheHis: 0.654 ± 0.019
0.909PheIle: 0.909 ± 0.023
0.544PheLys: 0.544 ± 0.019
2.635PheLeu: 2.635 ± 0.047
0.431PheMet: 0.431 ± 0.016
0.589PheAsn: 0.589 ± 0.022
1.492PhePro: 1.492 ± 0.033
0.748PheGln: 0.748 ± 0.021
1.874PheArg: 1.874 ± 0.035
1.557PheSer: 1.557 ± 0.035
2.056PheThr: 2.056 ± 0.036
2.174PheVal: 2.174 ± 0.038
0.444PheTrp: 0.444 ± 0.019
0.611PheTyr: 0.611 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.796GlyAla: 10.796 ± 0.089
0.844GlyCys: 0.844 ± 0.023
5.184GlyAsp: 5.184 ± 0.06
5.255GlyGlu: 5.255 ± 0.063
2.962GlyPhe: 2.962 ± 0.047
8.908GlyGly: 8.908 ± 0.094
2.494GlyHis: 2.494 ± 0.047
3.686GlyIle: 3.686 ± 0.041
2.68GlyLys: 2.68 ± 0.051
9.395GlyLeu: 9.395 ± 0.095
2.206GlyMet: 2.206 ± 0.034
1.708GlyAsn: 1.708 ± 0.04
5.083GlyPro: 5.083 ± 0.064
2.785GlyGln: 2.785 ± 0.043
7.589GlyArg: 7.589 ± 0.07
5.385GlySer: 5.385 ± 0.068
5.962GlyThr: 5.962 ± 0.071
7.573GlyVal: 7.573 ± 0.077
1.695GlyTrp: 1.695 ± 0.034
2.402GlyTyr: 2.402 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.625HisAla: 2.625 ± 0.045
0.212HisCys: 0.212 ± 0.011
1.378HisAsp: 1.378 ± 0.034
1.25HisGlu: 1.25 ± 0.028
0.665HisPhe: 0.665 ± 0.023
2.474HisGly: 2.474 ± 0.044
0.733HisHis: 0.733 ± 0.025
0.789HisIle: 0.789 ± 0.024
0.392HisLys: 0.392 ± 0.016
2.453HisLeu: 2.453 ± 0.043
0.389HisMet: 0.389 ± 0.016
0.376HisAsn: 0.376 ± 0.012
1.896HisPro: 1.896 ± 0.039
0.719HisGln: 0.719 ± 0.022
2.108HisArg: 2.108 ± 0.04
1.059HisSer: 1.059 ± 0.028
1.461HisThr: 1.461 ± 0.034
1.619HisVal: 1.619 ± 0.034
0.381HisTrp: 0.381 ± 0.017
0.493HisTyr: 0.493 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.243IleAla: 5.243 ± 0.063
0.346IleCys: 0.346 ± 0.014
2.437IleAsp: 2.437 ± 0.037
2.285IleGlu: 2.285 ± 0.045
0.828IlePhe: 0.828 ± 0.024
3.945IleGly: 3.945 ± 0.057
0.683IleHis: 0.683 ± 0.018
0.986IleIle: 0.986 ± 0.026
0.856IleLys: 0.856 ± 0.025
2.554IleLeu: 2.554 ± 0.041
0.516IleMet: 0.516 ± 0.02
0.772IleAsn: 0.772 ± 0.021
1.922IlePro: 1.922 ± 0.036
0.775IleGln: 0.775 ± 0.021
2.451IleArg: 2.451 ± 0.038
1.929IleSer: 1.929 ± 0.037
2.38IleThr: 2.38 ± 0.041
2.95IleVal: 2.95 ± 0.044
0.42IleTrp: 0.42 ± 0.015
0.597IleTyr: 0.597 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
3.101LysAla: 3.101 ± 0.062
0.12LysCys: 0.12 ± 0.01
1.449LysAsp: 1.449 ± 0.041
1.293LysGlu: 1.293 ± 0.03
0.461LysPhe: 0.461 ± 0.019
2.041LysGly: 2.041 ± 0.042
0.448LysHis: 0.448 ± 0.018
0.916LysIle: 0.916 ± 0.029
1.008LysLys: 1.008 ± 0.037
2.157LysLeu: 2.157 ± 0.038
0.414LysMet: 0.414 ± 0.017
0.584LysAsn: 0.584 ± 0.022
1.457LysPro: 1.457 ± 0.032
0.74LysGln: 0.74 ± 0.026
1.603LysArg: 1.603 ± 0.036
1.256LysSer: 1.256 ± 0.037
1.351LysThr: 1.351 ± 0.034
2.066LysVal: 2.066 ± 0.041
0.269LysTrp: 0.269 ± 0.014
0.455LysTyr: 0.455 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
14.048LeuAla: 14.048 ± 0.115
0.835LeuCys: 0.835 ± 0.023
6.67LeuAsp: 6.67 ± 0.071
5.0LeuGlu: 5.0 ± 0.067
2.76LeuPhe: 2.76 ± 0.043
9.263LeuGly: 9.263 ± 0.084
2.226LeuHis: 2.226 ± 0.039
3.73LeuIle: 3.73 ± 0.05
2.07LeuLys: 2.07 ± 0.041
10.897LeuLeu: 10.897 ± 0.108
1.796LeuMet: 1.796 ± 0.031
1.685LeuAsn: 1.685 ± 0.033
6.348LeuPro: 6.348 ± 0.062
2.106LeuGln: 2.106 ± 0.038
8.729LeuArg: 8.729 ± 0.093
5.594LeuSer: 5.594 ± 0.06
6.813LeuThr: 6.813 ± 0.063
8.517LeuVal: 8.517 ± 0.082
1.328LeuTrp: 1.328 ± 0.03
1.898LeuTyr: 1.898 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
2.586MetAla: 2.586 ± 0.042
0.142MetCys: 0.142 ± 0.009
1.02MetAsp: 1.02 ± 0.03
0.844MetGlu: 0.844 ± 0.023
0.486MetPhe: 0.486 ± 0.018
1.503MetGly: 1.503 ± 0.035
0.358MetHis: 0.358 ± 0.014
0.714MetIle: 0.714 ± 0.024
0.452MetLys: 0.452 ± 0.018
1.816MetLeu: 1.816 ± 0.031
0.325MetMet: 0.325 ± 0.015
0.452MetAsn: 0.452 ± 0.016
1.17MetPro: 1.17 ± 0.026
0.406MetGln: 0.406 ± 0.018
1.615MetArg: 1.615 ± 0.034
1.433MetSer: 1.433 ± 0.031
1.584MetThr: 1.584 ± 0.029
1.485MetVal: 1.485 ± 0.029
0.214MetTrp: 0.214 ± 0.012
0.361MetTyr: 0.361 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.265AsnAla: 2.265 ± 0.041
0.179AsnCys: 0.179 ± 0.011
0.977AsnAsp: 0.977 ± 0.025
0.847AsnGlu: 0.847 ± 0.023
0.487AsnPhe: 0.487 ± 0.019
1.97AsnGly: 1.97 ± 0.031
0.396AsnHis: 0.396 ± 0.017
0.663AsnIle: 0.663 ± 0.021
0.441AsnLys: 0.441 ± 0.02
1.671AsnLeu: 1.671 ± 0.03
0.315AsnMet: 0.315 ± 0.014
0.427AsnAsn: 0.427 ± 0.017
1.367AsnPro: 1.367 ± 0.029
0.525AsnGln: 0.525 ± 0.019
1.293AsnArg: 1.293 ± 0.031
0.907AsnSer: 0.907 ± 0.027
1.146AsnThr: 1.146 ± 0.028
1.434AsnVal: 1.434 ± 0.028
0.273AsnTrp: 0.273 ± 0.013
0.443AsnTyr: 0.443 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
7.646ProAla: 7.646 ± 0.076
0.357ProCys: 0.357 ± 0.016
4.48ProAsp: 4.48 ± 0.057
4.519ProGlu: 4.519 ± 0.054
1.576ProPhe: 1.576 ± 0.033
6.443ProGly: 6.443 ± 0.071
1.46ProHis: 1.46 ± 0.032
1.51ProIle: 1.51 ± 0.031
1.336ProLys: 1.336 ± 0.034
5.361ProLeu: 5.361 ± 0.063
1.102ProMet: 1.102 ± 0.027
0.906ProAsn: 0.906 ± 0.026
3.264ProPro: 3.264 ± 0.052
1.674ProGln: 1.674 ± 0.043
4.029ProArg: 4.029 ± 0.057
3.236ProSer: 3.236 ± 0.051
3.149ProThr: 3.149 ± 0.048
5.102ProVal: 5.102 ± 0.052
0.894ProTrp: 0.894 ± 0.024
1.457ProTyr: 1.457 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.607GlnAla: 3.607 ± 0.05
0.173GlnCys: 0.173 ± 0.011
1.423GlnAsp: 1.423 ± 0.03
1.511GlnGlu: 1.511 ± 0.03
0.686GlnPhe: 0.686 ± 0.019
2.336GlnGly: 2.336 ± 0.043
0.651GlnHis: 0.651 ± 0.02
1.147GlnIle: 1.147 ± 0.025
0.654GlnLys: 0.654 ± 0.02
2.979GlnLeu: 2.979 ± 0.038
0.579GlnMet: 0.579 ± 0.019
0.525GlnAsn: 0.525 ± 0.02
1.662GlnPro: 1.662 ± 0.038
1.202GlnGln: 1.202 ± 0.045
2.5GlnArg: 2.5 ± 0.042
1.321GlnSer: 1.321 ± 0.031
1.371GlnThr: 1.371 ± 0.032
2.288GlnVal: 2.288 ± 0.041
0.422GlnTrp: 0.422 ± 0.019
0.595GlnTyr: 0.595 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
9.318ArgAla: 9.318 ± 0.086
0.604ArgCys: 0.604 ± 0.023
4.269ArgAsp: 4.269 ± 0.057
4.868ArgGlu: 4.868 ± 0.069
2.422ArgPhe: 2.422 ± 0.043
5.902ArgGly: 5.902 ± 0.067
2.18ArgHis: 2.18 ± 0.037
3.415ArgIle: 3.415 ± 0.048
1.802ArgLys: 1.802 ± 0.04
8.807ArgLeu: 8.807 ± 0.097
1.877ArgMet: 1.877 ± 0.032
1.429ArgAsn: 1.429 ± 0.032
4.896ArgPro: 4.896 ± 0.066
2.374ArgGln: 2.374 ± 0.042
7.408ArgArg: 7.408 ± 0.084
4.039ArgSer: 4.039 ± 0.05
5.211ArgThr: 5.211 ± 0.051
5.871ArgVal: 5.871 ± 0.067
1.395ArgTrp: 1.395 ± 0.028
1.868ArgTyr: 1.868 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.676SerAla: 6.676 ± 0.075
0.442SerCys: 0.442 ± 0.017
2.802SerAsp: 2.802 ± 0.047
2.547SerGlu: 2.547 ± 0.045
1.645SerPhe: 1.645 ± 0.034
6.184SerGly: 6.184 ± 0.07
1.086SerHis: 1.086 ± 0.029
1.569SerIle: 1.569 ± 0.033
1.135SerLys: 1.135 ± 0.033
4.892SerLeu: 4.892 ± 0.06
1.116SerMet: 1.116 ± 0.03
0.948SerAsn: 0.948 ± 0.021
3.207SerPro: 3.207 ± 0.048
1.301SerGln: 1.301 ± 0.03
3.762SerArg: 3.762 ± 0.048
2.982SerSer: 2.982 ± 0.062
2.995SerThr: 2.995 ± 0.044
4.236SerVal: 4.236 ± 0.055
0.92SerTrp: 0.92 ± 0.024
1.293SerTyr: 1.293 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
8.562ThrAla: 8.562 ± 0.085
0.404ThrCys: 0.404 ± 0.016
3.54ThrAsp: 3.54 ± 0.047
3.301ThrGlu: 3.301 ± 0.045
1.65ThrPhe: 1.65 ± 0.03
6.613ThrGly: 6.613 ± 0.07
1.218ThrHis: 1.218 ± 0.027
1.883ThrIle: 1.883 ± 0.036
1.257ThrLys: 1.257 ± 0.028
5.714ThrLeu: 5.714 ± 0.058
1.059ThrMet: 1.059 ± 0.026
0.982ThrAsn: 0.982 ± 0.027
3.955ThrPro: 3.955 ± 0.054
1.296ThrGln: 1.296 ± 0.029
3.811ThrArg: 3.811 ± 0.049
3.277ThrSer: 3.277 ± 0.047
3.842ThrThr: 3.842 ± 0.06
5.984ThrVal: 5.984 ± 0.061
0.864ThrTrp: 0.864 ± 0.02
1.317ThrTyr: 1.317 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.262ValAla: 10.262 ± 0.095
0.816ValCys: 0.816 ± 0.023
4.889ValAsp: 4.889 ± 0.057
4.787ValGlu: 4.787 ± 0.064
2.532ValPhe: 2.532 ± 0.045
6.38ValGly: 6.38 ± 0.073
1.941ValHis: 1.941 ± 0.038
3.179ValIle: 3.179 ± 0.056
1.731ValLys: 1.731 ± 0.037
9.207ValLeu: 9.207 ± 0.084
1.548ValMet: 1.548 ± 0.032
1.636ValAsn: 1.636 ± 0.029
4.944ValPro: 4.944 ± 0.058
2.007ValGln: 2.007 ± 0.036
7.01ValArg: 7.01 ± 0.075
4.435ValSer: 4.435 ± 0.055
5.269ValThr: 5.269 ± 0.058
7.84ValVal: 7.84 ± 0.089
1.104ValTrp: 1.104 ± 0.027
1.604ValTyr: 1.604 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.615TrpAla: 1.615 ± 0.031
0.152TrpCys: 0.152 ± 0.01
0.83TrpAsp: 0.83 ± 0.025
0.741TrpGlu: 0.741 ± 0.024
0.525TrpPhe: 0.525 ± 0.019
1.113TrpGly: 1.113 ± 0.026
0.357TrpHis: 0.357 ± 0.015
0.55TrpIle: 0.55 ± 0.019
0.371TrpLys: 0.371 ± 0.014
1.75TrpLeu: 1.75 ± 0.031
0.319TrpMet: 0.319 ± 0.016
0.422TrpAsn: 0.422 ± 0.016
0.774TrpPro: 0.774 ± 0.021
0.589TrpGln: 0.589 ± 0.022
1.305TrpArg: 1.305 ± 0.026
0.93TrpSer: 0.93 ± 0.026
0.971TrpThr: 0.971 ± 0.026
0.981TrpVal: 0.981 ± 0.022
0.31TrpTrp: 0.31 ± 0.016
0.357TrpTyr: 0.357 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.731TyrAla: 2.731 ± 0.039
0.182TyrCys: 0.182 ± 0.011
1.53TyrAsp: 1.53 ± 0.035
1.299TyrGlu: 1.299 ± 0.033
0.672TyrPhe: 0.672 ± 0.018
2.334TyrGly: 2.334 ± 0.043
0.438TyrHis: 0.438 ± 0.017
0.573TyrIle: 0.573 ± 0.02
0.436TyrLys: 0.436 ± 0.019
2.154TyrLeu: 2.154 ± 0.043
0.263TyrMet: 0.263 ± 0.013
0.444TyrAsn: 0.444 ± 0.017
1.11TyrPro: 1.11 ± 0.026
0.656TyrGln: 0.656 ± 0.021
1.893TyrArg: 1.893 ± 0.041
0.981TyrSer: 0.981 ± 0.026
1.224TyrThr: 1.224 ± 0.029
1.673TyrVal: 1.673 ± 0.038
0.384TyrTrp: 0.384 ± 0.015
0.471TyrTyr: 0.471 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5582 proteins (1629245 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski