Shewanella phage Thanatos-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5CAB3|A0A6M5CAB3_9CAUD dCMP deaminase OS=Shewanella phage Thanatos-1 OX=2734808 GN=TH1_119 PE=3 SV=1
MM1 pKa = 7.53TEE3 pKa = 3.84VTLQSAPSLDD13 pKa = 3.48TLIEE17 pKa = 4.22KK18 pKa = 10.33SQEE21 pKa = 3.9LCNLFAEE28 pKa = 5.09VKK30 pKa = 10.41ALANSMDD37 pKa = 3.66KK38 pKa = 10.79DD39 pKa = 3.42IEE41 pKa = 4.48FNCNYY46 pKa = 10.37GEE48 pKa = 4.2NEE50 pKa = 4.16VKK52 pKa = 9.15ITEE55 pKa = 4.44WNSSACYY62 pKa = 10.53GEE64 pKa = 5.62DD65 pKa = 3.92EE66 pKa = 4.26ITTVNTEE73 pKa = 4.57SVWHH77 pKa = 6.42SSNCC81 pKa = 3.51

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5CC30|A0A6M5CC30_9CAUD Uncharacterized protein OS=Shewanella phage Thanatos-1 OX=2734808 GN=TH1_065 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.28LRR4 pKa = 11.84YY5 pKa = 9.06KK6 pKa = 10.29QRR8 pKa = 11.84QLLEE12 pKa = 4.79LIDD15 pKa = 3.72RR16 pKa = 11.84GYY18 pKa = 10.5EE19 pKa = 3.91FKK21 pKa = 11.19VGLNSKK27 pKa = 10.44GPTLQGIPNYY37 pKa = 10.67NPTLYY42 pKa = 9.61ATLTMRR48 pKa = 11.84SLIKK52 pKa = 10.17RR53 pKa = 11.84KK54 pKa = 9.29MIIIEE59 pKa = 4.18GNTLKK64 pKa = 10.82RR65 pKa = 11.84ILL67 pKa = 3.98

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

0

206

51209

36

1259

248.6

28.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.687 ± 0.194

1.129 ± 0.066

5.968 ± 0.108

6.854 ± 0.164

4.267 ± 0.11

5.751 ± 0.209

1.806 ± 0.079

7.51 ± 0.102

7.788 ± 0.204

8.87 ± 0.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.086

5.841 ± 0.125

3.693 ± 0.098

3.101 ± 0.103

3.972 ± 0.1

7.639 ± 0.138

5.87 ± 0.174

6.153 ± 0.124

1.176 ± 0.042

4.306 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski