Amino acid dipepetide frequency for Shewanella phage Thanatos-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.398AlaAla: 3.398 ± 0.342
0.41AlaCys: 0.41 ± 0.094
3.007AlaAsp: 3.007 ± 0.206
3.398AlaGlu: 3.398 ± 0.367
2.031AlaPhe: 2.031 ± 0.211
3.007AlaGly: 3.007 ± 0.389
0.781AlaHis: 0.781 ± 0.128
4.765AlaIle: 4.765 ± 0.312
4.843AlaLys: 4.843 ± 0.293
6.269AlaLeu: 6.269 ± 0.378
1.543AlaMet: 1.543 ± 0.175
3.085AlaAsn: 3.085 ± 0.303
2.05AlaPro: 2.05 ± 0.24
1.797AlaGln: 1.797 ± 0.198
2.363AlaArg: 2.363 ± 0.232
3.945AlaSer: 3.945 ± 0.258
3.652AlaThr: 3.652 ± 0.512
3.261AlaVal: 3.261 ± 0.312
0.644AlaTrp: 0.644 ± 0.11
2.617AlaTyr: 2.617 ± 0.209
0.0AlaXaa: 0.0 ± 0.0
Cys
0.566CysAla: 0.566 ± 0.12
0.117CysCys: 0.117 ± 0.046
0.547CysAsp: 0.547 ± 0.095
0.781CysGlu: 0.781 ± 0.13
0.469CysPhe: 0.469 ± 0.087
0.703CysGly: 0.703 ± 0.124
0.293CysHis: 0.293 ± 0.077
0.859CysIle: 0.859 ± 0.143
0.762CysLys: 0.762 ± 0.144
0.918CysLeu: 0.918 ± 0.145
0.156CysMet: 0.156 ± 0.054
0.801CysAsn: 0.801 ± 0.125
0.469CysPro: 0.469 ± 0.087
0.273CysGln: 0.273 ± 0.076
0.547CysArg: 0.547 ± 0.098
0.937CysSer: 0.937 ± 0.116
0.605CysThr: 0.605 ± 0.107
0.723CysVal: 0.723 ± 0.131
0.117CysTrp: 0.117 ± 0.045
0.644CysTyr: 0.644 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
2.675AspAla: 2.675 ± 0.244
0.566AspCys: 0.566 ± 0.096
3.3AspAsp: 3.3 ± 0.314
3.867AspGlu: 3.867 ± 0.337
3.456AspPhe: 3.456 ± 0.237
3.788AspGly: 3.788 ± 0.277
0.84AspHis: 0.84 ± 0.117
4.863AspIle: 4.863 ± 0.288
4.472AspLys: 4.472 ± 0.294
5.663AspLeu: 5.663 ± 0.303
1.66AspMet: 1.66 ± 0.18
2.988AspAsn: 2.988 ± 0.234
2.363AspPro: 2.363 ± 0.203
1.445AspGln: 1.445 ± 0.156
2.187AspArg: 2.187 ± 0.194
5.175AspSer: 5.175 ± 0.302
2.871AspThr: 2.871 ± 0.235
3.476AspVal: 3.476 ± 0.241
0.976AspTrp: 0.976 ± 0.157
3.046AspTyr: 3.046 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
3.593GluAla: 3.593 ± 0.348
0.918GluCys: 0.918 ± 0.144
3.515GluAsp: 3.515 ± 0.254
4.804GluGlu: 4.804 ± 0.338
3.144GluPhe: 3.144 ± 0.243
3.828GluGly: 3.828 ± 0.283
1.445GluHis: 1.445 ± 0.156
5.566GluIle: 5.566 ± 0.291
4.355GluLys: 4.355 ± 0.335
7.792GluLeu: 7.792 ± 0.445
2.129GluMet: 2.129 ± 0.236
3.984GluAsn: 3.984 ± 0.274
1.679GluPro: 1.679 ± 0.162
2.05GluGln: 2.05 ± 0.251
2.421GluArg: 2.421 ± 0.207
5.526GluSer: 5.526 ± 0.443
3.613GluThr: 3.613 ± 0.355
3.906GluVal: 3.906 ± 0.265
0.801GluTrp: 0.801 ± 0.124
3.476GluTyr: 3.476 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
1.836PheAla: 1.836 ± 0.191
0.469PheCys: 0.469 ± 0.105
3.32PheAsp: 3.32 ± 0.3
2.851PheGlu: 2.851 ± 0.224
1.308PhePhe: 1.308 ± 0.177
2.441PheGly: 2.441 ± 0.282
0.43PheHis: 0.43 ± 0.096
2.988PheIle: 2.988 ± 0.294
3.847PheLys: 3.847 ± 0.312
3.144PheLeu: 3.144 ± 0.256
1.25PheMet: 1.25 ± 0.166
3.222PheAsn: 3.222 ± 0.253
1.211PhePro: 1.211 ± 0.146
1.074PheGln: 1.074 ± 0.152
1.797PheArg: 1.797 ± 0.178
3.886PheSer: 3.886 ± 0.276
2.636PheThr: 2.636 ± 0.208
2.578PheVal: 2.578 ± 0.206
0.43PheTrp: 0.43 ± 0.096
1.933PheTyr: 1.933 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
3.496GlyAla: 3.496 ± 0.38
0.547GlyCys: 0.547 ± 0.107
3.456GlyAsp: 3.456 ± 0.262
3.515GlyGlu: 3.515 ± 0.293
2.597GlyPhe: 2.597 ± 0.233
2.773GlyGly: 2.773 ± 0.308
0.957GlyHis: 0.957 ± 0.128
4.765GlyIle: 4.765 ± 0.318
4.238GlyLys: 4.238 ± 0.363
5.448GlyLeu: 5.448 ± 0.333
1.64GlyMet: 1.64 ± 0.204
3.437GlyAsn: 3.437 ± 0.293
1.387GlyPro: 1.387 ± 0.157
2.304GlyGln: 2.304 ± 0.248
2.187GlyArg: 2.187 ± 0.179
4.179GlySer: 4.179 ± 0.374
3.769GlyThr: 3.769 ± 0.349
3.671GlyVal: 3.671 ± 0.334
0.664GlyTrp: 0.664 ± 0.14
2.48GlyTyr: 2.48 ± 0.229
0.0GlyXaa: 0.0 ± 0.0
His
0.762HisAla: 0.762 ± 0.125
0.234HisCys: 0.234 ± 0.077
0.879HisAsp: 0.879 ± 0.147
1.015HisGlu: 1.015 ± 0.138
0.898HisPhe: 0.898 ± 0.135
0.762HisGly: 0.762 ± 0.126
0.352HisHis: 0.352 ± 0.083
1.504HisIle: 1.504 ± 0.154
1.484HisLys: 1.484 ± 0.168
1.621HisLeu: 1.621 ± 0.18
0.547HisMet: 0.547 ± 0.085
1.133HisAsn: 1.133 ± 0.145
0.781HisPro: 0.781 ± 0.125
0.547HisGln: 0.547 ± 0.092
0.781HisArg: 0.781 ± 0.121
1.679HisSer: 1.679 ± 0.176
0.937HisThr: 0.937 ± 0.171
1.152HisVal: 1.152 ± 0.132
0.215HisTrp: 0.215 ± 0.067
0.781HisTyr: 0.781 ± 0.121
0.0HisXaa: 0.0 ± 0.0
Ile
4.472IleAla: 4.472 ± 0.352
1.504IleCys: 1.504 ± 0.189
5.312IleAsp: 5.312 ± 0.312
5.097IleGlu: 5.097 ± 0.298
2.461IlePhe: 2.461 ± 0.21
3.945IleGly: 3.945 ± 0.325
1.347IleHis: 1.347 ± 0.167
4.843IleIle: 4.843 ± 0.296
6.542IleLys: 6.542 ± 0.437
6.151IleLeu: 6.151 ± 0.387
1.523IleMet: 1.523 ± 0.187
4.687IleAsn: 4.687 ± 0.287
3.027IlePro: 3.027 ± 0.247
2.617IleGln: 2.617 ± 0.256
3.027IleArg: 3.027 ± 0.205
5.819IleSer: 5.819 ± 0.364
4.296IleThr: 4.296 ± 0.253
4.609IleVal: 4.609 ± 0.302
0.703IleTrp: 0.703 ± 0.107
3.125IleTyr: 3.125 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
5.526LysAla: 5.526 ± 0.319
0.781LysCys: 0.781 ± 0.14
4.999LysAsp: 4.999 ± 0.346
6.151LysGlu: 6.151 ± 0.416
4.081LysPhe: 4.081 ± 0.306
3.867LysGly: 3.867 ± 0.289
1.582LysHis: 1.582 ± 0.173
5.39LysIle: 5.39 ± 0.353
4.628LysLys: 4.628 ± 0.351
6.64LysLeu: 6.64 ± 0.409
2.539LysMet: 2.539 ± 0.226
4.003LysAsn: 4.003 ± 0.242
2.968LysPro: 2.968 ± 0.278
2.363LysGln: 2.363 ± 0.222
2.617LysArg: 2.617 ± 0.241
5.253LysSer: 5.253 ± 0.305
4.999LysThr: 4.999 ± 0.321
5.038LysVal: 5.038 ± 0.306
1.23LysTrp: 1.23 ± 0.166
3.222LysTyr: 3.222 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
5.468LeuAla: 5.468 ± 0.355
0.957LeuCys: 0.957 ± 0.145
6.327LeuAsp: 6.327 ± 0.448
7.382LeuGlu: 7.382 ± 0.444
3.574LeuPhe: 3.574 ± 0.244
4.765LeuGly: 4.765 ± 0.34
1.562LeuHis: 1.562 ± 0.182
5.624LeuIle: 5.624 ± 0.343
7.401LeuLys: 7.401 ± 0.402
6.835LeuLeu: 6.835 ± 0.489
2.207LeuMet: 2.207 ± 0.195
5.448LeuAsn: 5.448 ± 0.306
3.359LeuPro: 3.359 ± 0.207
2.871LeuGln: 2.871 ± 0.254
4.003LeuArg: 4.003 ± 0.259
7.011LeuSer: 7.011 ± 0.422
4.687LeuThr: 4.687 ± 0.368
4.765LeuVal: 4.765 ± 0.294
1.015LeuTrp: 1.015 ± 0.131
3.437LeuTyr: 3.437 ± 0.239
0.0LeuXaa: 0.0 ± 0.0
Met
1.933MetAla: 1.933 ± 0.179
0.254MetCys: 0.254 ± 0.065
1.679MetAsp: 1.679 ± 0.178
1.66MetGlu: 1.66 ± 0.176
1.25MetPhe: 1.25 ± 0.162
1.191MetGly: 1.191 ± 0.161
0.527MetHis: 0.527 ± 0.095
1.894MetIle: 1.894 ± 0.196
1.66MetLys: 1.66 ± 0.164
2.324MetLeu: 2.324 ± 0.191
0.508MetMet: 0.508 ± 0.109
1.699MetAsn: 1.699 ± 0.189
1.152MetPro: 1.152 ± 0.121
0.976MetGln: 0.976 ± 0.119
0.781MetArg: 0.781 ± 0.127
2.168MetSer: 2.168 ± 0.19
1.504MetThr: 1.504 ± 0.165
1.64MetVal: 1.64 ± 0.162
0.293MetTrp: 0.293 ± 0.069
1.113MetTyr: 1.113 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
3.339AsnAla: 3.339 ± 0.242
0.82AsnCys: 0.82 ± 0.15
3.066AsnAsp: 3.066 ± 0.235
3.066AsnGlu: 3.066 ± 0.197
2.558AsnPhe: 2.558 ± 0.261
3.691AsnGly: 3.691 ± 0.308
1.211AsnHis: 1.211 ± 0.135
4.882AsnIle: 4.882 ± 0.292
4.57AsnLys: 4.57 ± 0.297
4.999AsnLeu: 4.999 ± 0.351
1.562AsnMet: 1.562 ± 0.177
3.417AsnAsn: 3.417 ± 0.274
2.656AsnPro: 2.656 ± 0.246
1.679AsnGln: 1.679 ± 0.227
2.539AsnArg: 2.539 ± 0.208
4.804AsnSer: 4.804 ± 0.309
3.613AsnThr: 3.613 ± 0.282
2.968AsnVal: 2.968 ± 0.225
0.605AsnTrp: 0.605 ± 0.092
2.363AsnTyr: 2.363 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
1.797ProAla: 1.797 ± 0.23
0.371ProCys: 0.371 ± 0.078
2.07ProAsp: 2.07 ± 0.194
2.988ProGlu: 2.988 ± 0.213
1.25ProPhe: 1.25 ± 0.153
2.695ProGly: 2.695 ± 0.258
0.605ProHis: 0.605 ± 0.106
2.402ProIle: 2.402 ± 0.217
3.456ProLys: 3.456 ± 0.306
2.812ProLeu: 2.812 ± 0.223
0.508ProMet: 0.508 ± 0.101
1.855ProAsn: 1.855 ± 0.198
1.074ProPro: 1.074 ± 0.148
0.937ProGln: 0.937 ± 0.137
1.367ProArg: 1.367 ± 0.196
2.988ProSer: 2.988 ± 0.219
2.597ProThr: 2.597 ± 0.204
2.988ProVal: 2.988 ± 0.225
0.586ProTrp: 0.586 ± 0.125
1.582ProTyr: 1.582 ± 0.18
0.0ProXaa: 0.0 ± 0.0
Gln
1.953GlnAla: 1.953 ± 0.199
0.254GlnCys: 0.254 ± 0.08
1.738GlnAsp: 1.738 ± 0.167
2.285GlnGlu: 2.285 ± 0.252
1.25GlnPhe: 1.25 ± 0.162
1.621GlnGly: 1.621 ± 0.166
0.527GlnHis: 0.527 ± 0.121
2.519GlnIle: 2.519 ± 0.234
2.148GlnLys: 2.148 ± 0.235
2.773GlnLeu: 2.773 ± 0.236
0.898GlnMet: 0.898 ± 0.14
1.406GlnAsn: 1.406 ± 0.146
0.898GlnPro: 0.898 ± 0.124
1.152GlnGln: 1.152 ± 0.154
1.055GlnArg: 1.055 ± 0.133
2.187GlnSer: 2.187 ± 0.202
2.285GlnThr: 2.285 ± 0.236
2.168GlnVal: 2.168 ± 0.216
0.488GlnTrp: 0.488 ± 0.095
1.406GlnTyr: 1.406 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
2.714ArgAla: 2.714 ± 0.258
0.43ArgCys: 0.43 ± 0.085
2.07ArgAsp: 2.07 ± 0.237
2.5ArgGlu: 2.5 ± 0.227
1.855ArgPhe: 1.855 ± 0.192
2.363ArgGly: 2.363 ± 0.222
0.781ArgHis: 0.781 ± 0.117
3.183ArgIle: 3.183 ± 0.292
3.378ArgLys: 3.378 ± 0.218
3.691ArgLeu: 3.691 ± 0.218
1.191ArgMet: 1.191 ± 0.146
2.226ArgAsn: 2.226 ± 0.195
1.523ArgPro: 1.523 ± 0.167
1.308ArgGln: 1.308 ± 0.147
1.562ArgArg: 1.562 ± 0.197
2.246ArgSer: 2.246 ± 0.194
2.07ArgThr: 2.07 ± 0.202
2.597ArgVal: 2.597 ± 0.251
0.41ArgTrp: 0.41 ± 0.083
1.621ArgTyr: 1.621 ± 0.153
0.0ArgXaa: 0.0 ± 0.0
Ser
3.417SerAla: 3.417 ± 0.284
0.625SerCys: 0.625 ± 0.131
3.808SerAsp: 3.808 ± 0.295
5.097SerGlu: 5.097 ± 0.393
3.066SerPhe: 3.066 ± 0.244
5.351SerGly: 5.351 ± 0.465
1.269SerHis: 1.269 ± 0.155
6.698SerIle: 6.698 ± 0.398
6.21SerLys: 6.21 ± 0.384
6.679SerLeu: 6.679 ± 0.355
1.523SerMet: 1.523 ± 0.165
4.394SerAsn: 4.394 ± 0.317
2.363SerPro: 2.363 ± 0.201
2.246SerGln: 2.246 ± 0.183
3.554SerArg: 3.554 ± 0.308
6.308SerSer: 6.308 ± 0.489
5.195SerThr: 5.195 ± 0.372
5.116SerVal: 5.116 ± 0.274
0.644SerTrp: 0.644 ± 0.109
2.832SerTyr: 2.832 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
3.3ThrAla: 3.3 ± 0.327
0.43ThrCys: 0.43 ± 0.093
3.144ThrAsp: 3.144 ± 0.261
4.277ThrGlu: 4.277 ± 0.322
2.285ThrPhe: 2.285 ± 0.265
4.081ThrGly: 4.081 ± 0.376
1.269ThrHis: 1.269 ± 0.161
4.374ThrIle: 4.374 ± 0.33
4.628ThrLys: 4.628 ± 0.31
5.8ThrLeu: 5.8 ± 0.349
1.621ThrMet: 1.621 ± 0.179
3.105ThrAsn: 3.105 ± 0.247
2.617ThrPro: 2.617 ± 0.323
1.953ThrGln: 1.953 ± 0.24
2.441ThrArg: 2.441 ± 0.22
3.515ThrSer: 3.515 ± 0.275
3.105ThrThr: 3.105 ± 0.273
3.906ThrVal: 3.906 ± 0.312
0.723ThrTrp: 0.723 ± 0.118
2.129ThrTyr: 2.129 ± 0.188
0.0ThrXaa: 0.0 ± 0.0
Val
3.574ValAla: 3.574 ± 0.273
0.82ValCys: 0.82 ± 0.122
4.14ValAsp: 4.14 ± 0.298
4.511ValGlu: 4.511 ± 0.316
2.656ValPhe: 2.656 ± 0.236
3.261ValGly: 3.261 ± 0.296
1.035ValHis: 1.035 ± 0.171
4.14ValIle: 4.14 ± 0.255
5.585ValLys: 5.585 ± 0.362
4.921ValLeu: 4.921 ± 0.366
1.523ValMet: 1.523 ± 0.153
4.003ValAsn: 4.003 ± 0.287
2.304ValPro: 2.304 ± 0.19
1.836ValGln: 1.836 ± 0.193
2.558ValArg: 2.558 ± 0.196
4.16ValSer: 4.16 ± 0.294
3.242ValThr: 3.242 ± 0.269
3.828ValVal: 3.828 ± 0.346
0.527ValTrp: 0.527 ± 0.089
2.91ValTyr: 2.91 ± 0.257
0.0ValXaa: 0.0 ± 0.0
Trp
0.683TrpAla: 0.683 ± 0.093
0.156TrpCys: 0.156 ± 0.051
0.84TrpAsp: 0.84 ± 0.116
0.937TrpGlu: 0.937 ± 0.162
0.605TrpPhe: 0.605 ± 0.098
0.371TrpGly: 0.371 ± 0.095
0.215TrpHis: 0.215 ± 0.069
0.84TrpIle: 0.84 ± 0.119
0.82TrpLys: 0.82 ± 0.127
0.859TrpLeu: 0.859 ± 0.115
0.41TrpMet: 0.41 ± 0.09
0.762TrpAsn: 0.762 ± 0.108
0.644TrpPro: 0.644 ± 0.116
0.293TrpGln: 0.293 ± 0.078
0.449TrpArg: 0.449 ± 0.079
0.742TrpSer: 0.742 ± 0.109
0.781TrpThr: 0.781 ± 0.122
0.781TrpVal: 0.781 ± 0.104
0.117TrpTrp: 0.117 ± 0.048
0.449TrpTyr: 0.449 ± 0.095
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.363TyrAla: 2.363 ± 0.281
0.625TyrCys: 0.625 ± 0.111
2.461TyrAsp: 2.461 ± 0.258
2.363TyrGlu: 2.363 ± 0.216
1.875TyrPhe: 1.875 ± 0.188
3.007TyrGly: 3.007 ± 0.25
1.035TyrHis: 1.035 ± 0.141
3.007TyrIle: 3.007 ± 0.291
2.851TyrLys: 2.851 ± 0.299
3.359TyrLeu: 3.359 ± 0.24
1.25TyrMet: 1.25 ± 0.168
2.753TyrAsn: 2.753 ± 0.28
2.402TyrPro: 2.402 ± 0.219
1.289TyrGln: 1.289 ± 0.124
1.484TyrArg: 1.484 ± 0.181
3.867TyrSer: 3.867 ± 0.301
2.246TyrThr: 2.246 ± 0.189
2.363TyrVal: 2.363 ± 0.212
0.566TyrTrp: 0.566 ± 0.099
1.894TyrTyr: 1.894 ± 0.229
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 206 proteins (51209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski