Aureimonas sp. Leaf324

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Aurantimonadaceae; Aureimonas; unclassified Aureimonas

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5GG35|A0A0Q5GG35_9RHIZ Formate dehydrogenase OS=Aureimonas sp. Leaf324 OX=1736336 GN=ASF65_10815 PE=3 SV=1
MM1 pKa = 7.68AATPLTGSKK10 pKa = 9.52WGDD13 pKa = 3.24SGVAGTPAGAVTWSFDD29 pKa = 3.78TTGSRR34 pKa = 11.84YY35 pKa = 9.27YY36 pKa = 10.84AYY38 pKa = 10.41DD39 pKa = 4.58DD40 pKa = 4.66IIASEE45 pKa = 5.29GYY47 pKa = 9.9RR48 pKa = 11.84SLVRR52 pKa = 11.84QAFDD56 pKa = 2.6AWEE59 pKa = 4.07KK60 pKa = 10.14VAAIDD65 pKa = 3.87FVEE68 pKa = 4.67VGSGPAEE75 pKa = 3.85IQVGLDD81 pKa = 3.63AIDD84 pKa = 3.88GRR86 pKa = 11.84GGTIGQAVWYY96 pKa = 9.9SRR98 pKa = 11.84GSVTTHH104 pKa = 6.65AEE106 pKa = 3.74IAVDD110 pKa = 4.66SAEE113 pKa = 4.12TWSPFVPNGSNFFGMMVHH131 pKa = 7.2EE132 pKa = 5.08IGHH135 pKa = 6.79AIGLEE140 pKa = 4.08HH141 pKa = 7.31SDD143 pKa = 4.02DD144 pKa = 4.54PSSIMYY150 pKa = 8.6PIASAYY156 pKa = 8.76MAFSNEE162 pKa = 3.75DD163 pKa = 3.1LAAIQALYY171 pKa = 10.62GPAAVAGRR179 pKa = 11.84LLYY182 pKa = 8.99GTSAPDD188 pKa = 3.71LLVGASGNDD197 pKa = 3.13TVYY200 pKa = 10.94GFEE203 pKa = 4.47GADD206 pKa = 3.36NLFGYY211 pKa = 10.26AGNDD215 pKa = 4.03LIAGNQGNDD224 pKa = 3.43QIFGGQGNDD233 pKa = 2.56IVYY236 pKa = 10.49GGRR239 pKa = 11.84DD240 pKa = 3.21QDD242 pKa = 4.02TVSGNLGNDD251 pKa = 3.17TVYY254 pKa = 11.5GDD256 pKa = 4.37LGNDD260 pKa = 3.3TVYY263 pKa = 10.99GGQGDD268 pKa = 4.07DD269 pKa = 3.34LVYY272 pKa = 10.78GGRR275 pKa = 11.84GDD277 pKa = 4.72DD278 pKa = 4.57LVSGDD283 pKa = 4.74LGNDD287 pKa = 3.46TLVGGFGNDD296 pKa = 2.92WLAGRR301 pKa = 11.84AGADD305 pKa = 3.06VFVFEE310 pKa = 5.47AGQGDD315 pKa = 4.44DD316 pKa = 4.63VIVDD320 pKa = 4.08FNAAEE325 pKa = 4.48GDD327 pKa = 3.74RR328 pKa = 11.84LVLSGQTYY336 pKa = 6.85TTAEE340 pKa = 4.2VNGSEE345 pKa = 4.21VLTLSGGGTITLLGVGTAPLEE366 pKa = 4.33AAALAA371 pKa = 4.32

Molecular weight: 38.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5GFP8|A0A0Q5GFP8_9RHIZ Succinyl-diaminopimelate desuccinylase OS=Aureimonas sp. Leaf324 OX=1736336 GN=dapE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84KK29 pKa = 9.29VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3989

0

3989

1266772

41

2829

317.6

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.32 ± 0.053

0.731 ± 0.011

5.863 ± 0.033

5.919 ± 0.039

3.7 ± 0.023

8.991 ± 0.041

1.893 ± 0.019

4.796 ± 0.028

2.644 ± 0.035

10.213 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.017

2.244 ± 0.021

5.245 ± 0.031

2.743 ± 0.022

7.86 ± 0.039

5.308 ± 0.026

5.301 ± 0.024

7.745 ± 0.034

1.191 ± 0.014

1.965 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski