Amino acid dipepetide frequency for Aureimonas sp. Leaf324

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.304AlaAla: 19.304 ± 0.194
1.005AlaCys: 1.005 ± 0.035
7.646AlaAsp: 7.646 ± 0.081
8.534AlaGlu: 8.534 ± 0.095
4.924AlaPhe: 4.924 ± 0.065
11.903AlaGly: 11.903 ± 0.109
2.201AlaHis: 2.201 ± 0.04
6.594AlaIle: 6.594 ± 0.087
3.725AlaLys: 3.725 ± 0.058
14.38AlaLeu: 14.38 ± 0.143
3.741AlaMet: 3.741 ± 0.057
2.733AlaAsn: 2.733 ± 0.049
6.245AlaPro: 6.245 ± 0.091
3.376AlaGln: 3.376 ± 0.066
10.123AlaArg: 10.123 ± 0.103
7.146AlaSer: 7.146 ± 0.086
6.221AlaThr: 6.221 ± 0.068
9.589AlaVal: 9.589 ± 0.091
1.349AlaTrp: 1.349 ± 0.033
2.463AlaTyr: 2.463 ± 0.048
0.001AlaXaa: 0.001 ± 0.001
Cys
0.824CysAla: 0.824 ± 0.031
0.069CysCys: 0.069 ± 0.008
0.464CysAsp: 0.464 ± 0.018
0.44CysGlu: 0.44 ± 0.019
0.311CysPhe: 0.311 ± 0.017
0.838CysGly: 0.838 ± 0.026
0.189CysHis: 0.189 ± 0.013
0.328CysIle: 0.328 ± 0.016
0.117CysLys: 0.117 ± 0.009
0.768CysLeu: 0.768 ± 0.025
0.113CysMet: 0.113 ± 0.01
0.152CysAsn: 0.152 ± 0.01
0.364CysPro: 0.364 ± 0.016
0.184CysGln: 0.184 ± 0.013
0.624CysArg: 0.624 ± 0.024
0.373CysSer: 0.373 ± 0.018
0.331CysThr: 0.331 ± 0.016
0.589CysVal: 0.589 ± 0.022
0.091CysTrp: 0.091 ± 0.009
0.138CysTyr: 0.138 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.891AspAla: 7.891 ± 0.076
0.411AspCys: 0.411 ± 0.019
3.279AspAsp: 3.279 ± 0.069
3.953AspGlu: 3.953 ± 0.062
2.018AspPhe: 2.018 ± 0.036
5.776AspGly: 5.776 ± 0.09
1.177AspHis: 1.177 ± 0.036
2.712AspIle: 2.712 ± 0.048
1.37AspLys: 1.37 ± 0.036
6.608AspLeu: 6.608 ± 0.084
1.27AspMet: 1.27 ± 0.03
1.062AspAsn: 1.062 ± 0.034
3.608AspPro: 3.608 ± 0.049
1.484AspGln: 1.484 ± 0.033
5.028AspArg: 5.028 ± 0.063
1.67AspSer: 1.67 ± 0.038
2.695AspThr: 2.695 ± 0.052
4.339AspVal: 4.339 ± 0.064
0.948AspTrp: 0.948 ± 0.024
1.331AspTyr: 1.331 ± 0.035
0.001AspXaa: 0.001 ± 0.001
Glu
9.228GluAla: 9.228 ± 0.11
0.316GluCys: 0.316 ± 0.015
2.922GluAsp: 2.922 ± 0.048
3.289GluGlu: 3.289 ± 0.065
1.78GluPhe: 1.78 ± 0.04
4.974GluGly: 4.974 ± 0.059
1.089GluHis: 1.089 ± 0.03
3.585GluIle: 3.585 ± 0.054
1.871GluLys: 1.871 ± 0.044
5.037GluLeu: 5.037 ± 0.071
1.414GluMet: 1.414 ± 0.036
1.434GluAsn: 1.434 ± 0.039
3.08GluPro: 3.08 ± 0.061
1.484GluGln: 1.484 ± 0.036
5.976GluArg: 5.976 ± 0.083
2.004GluSer: 2.004 ± 0.04
4.123GluThr: 4.123 ± 0.059
4.047GluVal: 4.047 ± 0.066
0.673GluTrp: 0.673 ± 0.024
0.859GluTyr: 0.859 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.933PheAla: 4.933 ± 0.058
0.361PheCys: 0.361 ± 0.017
2.777PheAsp: 2.777 ± 0.047
2.284PheGlu: 2.284 ± 0.047
1.364PhePhe: 1.364 ± 0.037
3.877PheGly: 3.877 ± 0.059
0.723PheHis: 0.723 ± 0.021
1.372PheIle: 1.372 ± 0.036
0.785PheLys: 0.785 ± 0.026
3.529PheLeu: 3.529 ± 0.05
0.683PheMet: 0.683 ± 0.023
0.88PheAsn: 0.88 ± 0.029
1.555PhePro: 1.555 ± 0.038
1.033PheGln: 1.033 ± 0.029
2.555PheArg: 2.555 ± 0.044
2.041PheSer: 2.041 ± 0.038
1.812PheThr: 1.812 ± 0.035
3.139PheVal: 3.139 ± 0.062
0.493PheTrp: 0.493 ± 0.022
0.803PheTyr: 0.803 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.595GlyAla: 10.595 ± 0.095
0.77GlyCys: 0.77 ± 0.026
4.748GlyAsp: 4.748 ± 0.068
5.532GlyGlu: 5.532 ± 0.071
3.806GlyPhe: 3.806 ± 0.063
7.891GlyGly: 7.891 ± 0.104
1.868GlyHis: 1.868 ± 0.043
4.531GlyIle: 4.531 ± 0.063
2.562GlyLys: 2.562 ± 0.055
9.373GlyLeu: 9.373 ± 0.106
2.167GlyMet: 2.167 ± 0.043
2.054GlyAsn: 2.054 ± 0.072
3.734GlyPro: 3.734 ± 0.055
2.827GlyGln: 2.827 ± 0.052
7.521GlyArg: 7.521 ± 0.076
5.006GlySer: 5.006 ± 0.072
5.081GlyThr: 5.081 ± 0.072
6.514GlyVal: 6.514 ± 0.075
1.318GlyTrp: 1.318 ± 0.032
2.015GlyTyr: 2.015 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.367HisAla: 2.367 ± 0.046
0.204HisCys: 0.204 ± 0.014
1.138HisAsp: 1.138 ± 0.033
1.058HisGlu: 1.058 ± 0.028
0.756HisPhe: 0.756 ± 0.025
1.902HisGly: 1.902 ± 0.038
0.544HisHis: 0.544 ± 0.026
0.756HisIle: 0.756 ± 0.025
0.389HisLys: 0.389 ± 0.017
1.989HisLeu: 1.989 ± 0.046
0.448HisMet: 0.448 ± 0.019
0.331HisAsn: 0.331 ± 0.016
1.325HisPro: 1.325 ± 0.033
0.512HisGln: 0.512 ± 0.019
1.467HisArg: 1.467 ± 0.034
0.822HisSer: 0.822 ± 0.025
0.699HisThr: 0.699 ± 0.029
1.495HisVal: 1.495 ± 0.033
0.276HisTrp: 0.276 ± 0.016
0.448HisTyr: 0.448 ± 0.021
0.001HisXaa: 0.001 ± 0.001
Ile
7.156IleAla: 7.156 ± 0.081
0.427IleCys: 0.427 ± 0.02
3.413IleAsp: 3.413 ± 0.053
3.509IleGlu: 3.509 ± 0.059
1.488IlePhe: 1.488 ± 0.04
5.069IleGly: 5.069 ± 0.066
0.862IleHis: 0.862 ± 0.028
1.603IleIle: 1.603 ± 0.043
1.002IleLys: 1.002 ± 0.034
4.805IleLeu: 4.805 ± 0.077
0.806IleMet: 0.806 ± 0.026
1.064IleAsn: 1.064 ± 0.034
2.071IlePro: 2.071 ± 0.036
1.132IleGln: 1.132 ± 0.029
3.44IleArg: 3.44 ± 0.053
2.255IleSer: 2.255 ± 0.04
2.112IleThr: 2.112 ± 0.045
4.348IleVal: 4.348 ± 0.062
0.489IleTrp: 0.489 ± 0.021
0.907IleTyr: 0.907 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.822LysAla: 3.822 ± 0.068
0.101LysCys: 0.101 ± 0.009
1.409LysAsp: 1.409 ± 0.037
1.235LysGlu: 1.235 ± 0.036
0.646LysPhe: 0.646 ± 0.024
2.462LysGly: 2.462 ± 0.054
0.398LysHis: 0.398 ± 0.018
1.239LysIle: 1.239 ± 0.033
0.839LysLys: 0.839 ± 0.032
2.66LysLeu: 2.66 ± 0.049
0.546LysMet: 0.546 ± 0.021
0.643LysAsn: 0.643 ± 0.022
1.835LysPro: 1.835 ± 0.043
0.611LysGln: 0.611 ± 0.021
2.208LysArg: 2.208 ± 0.044
1.486LysSer: 1.486 ± 0.035
1.587LysThr: 1.587 ± 0.04
2.046LysVal: 2.046 ± 0.049
0.241LysTrp: 0.241 ± 0.013
0.426LysTyr: 0.426 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.58LeuAla: 14.58 ± 0.127
0.862LeuCys: 0.862 ± 0.024
6.517LeuAsp: 6.517 ± 0.08
5.263LeuGlu: 5.263 ± 0.061
3.657LeuPhe: 3.657 ± 0.06
8.884LeuGly: 8.884 ± 0.102
1.812LeuHis: 1.812 ± 0.038
4.072LeuIle: 4.072 ± 0.062
3.082LeuLys: 3.082 ± 0.053
9.5LeuLeu: 9.5 ± 0.134
2.163LeuMet: 2.163 ± 0.043
2.207LeuAsn: 2.207 ± 0.043
5.609LeuPro: 5.609 ± 0.069
2.685LeuGln: 2.685 ± 0.044
7.383LeuArg: 7.383 ± 0.091
6.837LeuSer: 6.837 ± 0.081
5.506LeuThr: 5.506 ± 0.059
8.58LeuVal: 8.58 ± 0.109
1.068LeuTrp: 1.068 ± 0.03
1.864LeuTyr: 1.864 ± 0.039
0.002LeuXaa: 0.002 ± 0.002
Met
3.093MetAla: 3.093 ± 0.057
0.124MetCys: 0.124 ± 0.011
1.185MetAsp: 1.185 ± 0.036
1.13MetGlu: 1.13 ± 0.03
0.605MetPhe: 0.605 ± 0.02
1.771MetGly: 1.771 ± 0.039
0.369MetHis: 0.369 ± 0.019
1.224MetIle: 1.224 ± 0.032
0.83MetLys: 0.83 ± 0.027
2.217MetLeu: 2.217 ± 0.045
0.602MetMet: 0.602 ± 0.025
0.677MetAsn: 0.677 ± 0.026
1.441MetPro: 1.441 ± 0.032
0.652MetGln: 0.652 ± 0.023
1.985MetArg: 1.985 ± 0.043
1.588MetSer: 1.588 ± 0.036
1.811MetThr: 1.811 ± 0.037
1.582MetVal: 1.582 ± 0.033
0.163MetTrp: 0.163 ± 0.011
0.238MetTyr: 0.238 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.968AsnAla: 2.968 ± 0.052
0.18AsnCys: 0.18 ± 0.012
1.259AsnAsp: 1.259 ± 0.038
1.166AsnGlu: 1.166 ± 0.033
0.817AsnPhe: 0.817 ± 0.026
2.296AsnGly: 2.296 ± 0.064
0.416AsnHis: 0.416 ± 0.018
1.058AsnIle: 1.058 ± 0.033
0.496AsnLys: 0.496 ± 0.023
2.276AsnLeu: 2.276 ± 0.05
0.503AsnMet: 0.503 ± 0.022
0.528AsnAsn: 0.528 ± 0.024
1.514AsnPro: 1.514 ± 0.033
0.642AsnGln: 0.642 ± 0.023
1.685AsnArg: 1.685 ± 0.042
0.957AsnSer: 0.957 ± 0.026
1.077AsnThr: 1.077 ± 0.033
1.767AsnVal: 1.767 ± 0.039
0.324AsnTrp: 0.324 ± 0.017
0.514AsnTyr: 0.514 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.043ProAla: 7.043 ± 0.094
0.279ProCys: 0.279 ± 0.015
3.855ProAsp: 3.855 ± 0.059
3.511ProGlu: 3.511 ± 0.059
2.081ProPhe: 2.081 ± 0.043
4.768ProGly: 4.768 ± 0.063
1.04ProHis: 1.04 ± 0.03
2.475ProIle: 2.475 ± 0.046
1.437ProLys: 1.437 ± 0.038
4.778ProLeu: 4.778 ± 0.064
1.196ProMet: 1.196 ± 0.032
1.273ProAsn: 1.273 ± 0.035
2.519ProPro: 2.519 ± 0.056
1.4ProGln: 1.4 ± 0.031
3.147ProArg: 3.147 ± 0.057
2.899ProSer: 2.899 ± 0.051
2.603ProThr: 2.603 ± 0.05
4.433ProVal: 4.433 ± 0.056
0.617ProTrp: 0.617 ± 0.024
1.096ProTyr: 1.096 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.852GlnAla: 3.852 ± 0.065
0.141GlnCys: 0.141 ± 0.011
1.342GlnAsp: 1.342 ± 0.033
1.245GlnGlu: 1.245 ± 0.03
0.927GlnPhe: 0.927 ± 0.03
2.179GlnGly: 2.179 ± 0.042
0.479GlnHis: 0.479 ± 0.017
1.633GlnIle: 1.633 ± 0.038
0.858GlnLys: 0.858 ± 0.026
2.371GlnLeu: 2.371 ± 0.045
0.738GlnMet: 0.738 ± 0.022
0.708GlnAsn: 0.708 ± 0.026
1.675GlnPro: 1.675 ± 0.04
0.883GlnGln: 0.883 ± 0.034
2.384GlnArg: 2.384 ± 0.044
1.642GlnSer: 1.642 ± 0.039
1.642GlnThr: 1.642 ± 0.04
1.98GlnVal: 1.98 ± 0.04
0.291GlnTrp: 0.291 ± 0.015
0.457GlnTyr: 0.457 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
8.966ArgAla: 8.966 ± 0.091
0.464ArgCys: 0.464 ± 0.017
4.431ArgAsp: 4.431 ± 0.062
4.875ArgGlu: 4.875 ± 0.066
3.431ArgPhe: 3.431 ± 0.057
5.317ArgGly: 5.317 ± 0.071
1.85ArgHis: 1.85 ± 0.035
4.375ArgIle: 4.375 ± 0.063
1.769ArgLys: 1.769 ± 0.033
9.027ArgLeu: 9.027 ± 0.102
2.06ArgMet: 2.06 ± 0.045
1.689ArgAsn: 1.689 ± 0.041
4.143ArgPro: 4.143 ± 0.064
2.63ArgGln: 2.63 ± 0.046
7.249ArgArg: 7.249 ± 0.084
4.323ArgSer: 4.323 ± 0.065
4.007ArgThr: 4.007 ± 0.059
5.275ArgVal: 5.275 ± 0.061
1.021ArgTrp: 1.021 ± 0.035
1.696ArgTyr: 1.696 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.524SerAla: 6.524 ± 0.086
0.341SerCys: 0.341 ± 0.018
3.013SerAsp: 3.013 ± 0.058
2.771SerGlu: 2.771 ± 0.049
2.232SerPhe: 2.232 ± 0.039
5.63SerGly: 5.63 ± 0.069
0.995SerHis: 0.995 ± 0.026
2.712SerIle: 2.712 ± 0.045
1.299SerLys: 1.299 ± 0.036
5.639SerLeu: 5.639 ± 0.076
1.225SerMet: 1.225 ± 0.034
1.229SerAsn: 1.229 ± 0.033
2.813SerPro: 2.813 ± 0.048
1.526SerGln: 1.526 ± 0.039
3.749SerArg: 3.749 ± 0.058
2.835SerSer: 2.835 ± 0.067
2.601SerThr: 2.601 ± 0.048
4.186SerVal: 4.186 ± 0.064
0.622SerTrp: 0.622 ± 0.023
1.139SerTyr: 1.139 ± 0.03
0.001SerXaa: 0.001 ± 0.001
Thr
6.383ThrAla: 6.383 ± 0.083
0.329ThrCys: 0.329 ± 0.017
2.852ThrAsp: 2.852 ± 0.051
2.567ThrGlu: 2.567 ± 0.049
2.027ThrPhe: 2.027 ± 0.046
5.231ThrGly: 5.231 ± 0.075
0.951ThrHis: 0.951 ± 0.026
2.985ThrIle: 2.985 ± 0.05
1.274ThrLys: 1.274 ± 0.034
6.005ThrLeu: 6.005 ± 0.075
1.143ThrMet: 1.143 ± 0.033
1.153ThrAsn: 1.153 ± 0.032
3.217ThrPro: 3.217 ± 0.054
1.389ThrGln: 1.389 ± 0.033
3.638ThrArg: 3.638 ± 0.056
2.708ThrSer: 2.708 ± 0.051
2.825ThrThr: 2.825 ± 0.061
4.662ThrVal: 4.662 ± 0.065
0.542ThrTrp: 0.542 ± 0.02
1.126ThrTyr: 1.126 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.224ValAla: 10.224 ± 0.111
0.612ValCys: 0.612 ± 0.023
4.527ValAsp: 4.527 ± 0.065
5.096ValGlu: 5.096 ± 0.067
2.867ValPhe: 2.867 ± 0.048
6.331ValGly: 6.331 ± 0.076
1.308ValHis: 1.308 ± 0.034
3.465ValIle: 3.465 ± 0.064
1.936ValLys: 1.936 ± 0.038
7.7ValLeu: 7.7 ± 0.099
1.762ValMet: 1.762 ± 0.039
1.791ValAsn: 1.791 ± 0.041
4.094ValPro: 4.094 ± 0.063
1.892ValGln: 1.892 ± 0.042
5.553ValArg: 5.553 ± 0.069
4.724ValSer: 4.724 ± 0.066
4.545ValThr: 4.545 ± 0.055
6.728ValVal: 6.728 ± 0.082
0.875ValTrp: 0.875 ± 0.027
1.423ValTyr: 1.423 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.089TrpAla: 1.089 ± 0.032
0.115TrpCys: 0.115 ± 0.01
0.562TrpAsp: 0.562 ± 0.021
0.502TrpGlu: 0.502 ± 0.019
0.497TrpPhe: 0.497 ± 0.018
0.806TrpGly: 0.806 ± 0.027
0.264TrpHis: 0.264 ± 0.015
0.587TrpIle: 0.587 ± 0.021
0.354TrpLys: 0.354 ± 0.017
1.451TrpLeu: 1.451 ± 0.041
0.352TrpMet: 0.352 ± 0.017
0.366TrpAsn: 0.366 ± 0.018
0.608TrpPro: 0.608 ± 0.024
0.461TrpGln: 0.461 ± 0.02
1.152TrpArg: 1.152 ± 0.032
0.803TrpSer: 0.803 ± 0.028
0.805TrpThr: 0.805 ± 0.027
0.684TrpVal: 0.684 ± 0.028
0.216TrpTrp: 0.216 ± 0.014
0.232TrpTyr: 0.232 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.041
0.196TyrCys: 0.196 ± 0.012
1.29TyrAsp: 1.29 ± 0.032
1.229TyrGlu: 1.229 ± 0.032
0.765TyrPhe: 0.765 ± 0.029
2.007TyrGly: 2.007 ± 0.047
0.391TyrHis: 0.391 ± 0.018
0.653TyrIle: 0.653 ± 0.024
0.445TyrLys: 0.445 ± 0.019
2.022TyrLeu: 2.022 ± 0.041
0.353TyrMet: 0.353 ± 0.019
0.467TyrAsn: 0.467 ± 0.021
0.999TyrPro: 0.999 ± 0.032
0.625TyrGln: 0.625 ± 0.022
1.73TyrArg: 1.73 ± 0.035
0.968TyrSer: 0.968 ± 0.027
0.923TyrThr: 0.923 ± 0.027
1.468TyrVal: 1.468 ± 0.033
0.291TyrTrp: 0.291 ± 0.015
0.472TyrTyr: 0.472 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.003XaaAla: 0.003 ± 0.002
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.001XaaPhe: 0.001 ± 0.001
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.001XaaTyr: 0.001 ± 0.001
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3989 proteins (1266772 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski