Labrenzia sp. VG12
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5332 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222F4J6|A0A222F4J6_9RHOB TetR family transcriptional regulator OS=Labrenzia sp. VG12 OX=2021862 GN=CHH27_11005 PE=4 SV=1
MM1 pKa = 7.81 TDD3 pKa = 3.04 QNSAKK8 pKa = 9.04 PASAAAPKK16 pKa = 10.21 PSFTNEE22 pKa = 3.89 QIAHH26 pKa = 5.81 QLTDD30 pKa = 3.49 NFWIRR35 pKa = 11.84 EE36 pKa = 4.32 GEE38 pKa = 4.08 PTRR41 pKa = 11.84 HH42 pKa = 5.86 FDD44 pKa = 3.27 IPVGGTITVNYY55 pKa = 10.32 AGLTQKK61 pKa = 10.76 GQFYY65 pKa = 10.75 AHH67 pKa = 6.8 YY68 pKa = 10.38 ALEE71 pKa = 4.28 LWSDD75 pKa = 3.29 VTGIQFVTTRR85 pKa = 11.84 GQADD89 pKa = 3.27 IMFDD93 pKa = 3.51 DD94 pKa = 4.49 WDD96 pKa = 3.93 SGAYY100 pKa = 9.37 AHH102 pKa = 7.13 SNLKK106 pKa = 10.35 SDD108 pKa = 3.76 GSIDD112 pKa = 3.46 QSFVNVSTSWISSDD126 pKa = 3.95 GYY128 pKa = 11.31 NLSNYY133 pKa = 9.79 SFQTYY138 pKa = 7.42 IHH140 pKa = 7.13 EE141 pKa = 4.6 IGHH144 pKa = 6.42 ALGLGHH150 pKa = 7.58 AGNYY154 pKa = 9.13 NGSADD159 pKa = 3.71 YY160 pKa = 11.15 GIDD163 pKa = 3.34 NDD165 pKa = 4.1 YY166 pKa = 11.84 ANDD169 pKa = 3.25 SWQATVMSYY178 pKa = 10.2 FSQTEE183 pKa = 3.84 NTAILADD190 pKa = 4.23 FAYY193 pKa = 10.65 VATPQVADD201 pKa = 3.39 VMAIRR206 pKa = 11.84 EE207 pKa = 4.47 LYY209 pKa = 10.42 GSSGDD214 pKa = 3.5 TRR216 pKa = 11.84 TSDD219 pKa = 3.16 TVYY222 pKa = 11.05 GDD224 pKa = 3.17 NGTAGSIMNHH234 pKa = 6.65 IIGNSTPTTYY244 pKa = 10.45 TIVDD248 pKa = 3.82 DD249 pKa = 5.21 GGTDD253 pKa = 3.42 TLNFADD259 pKa = 4.17 TGQDD263 pKa = 3.01 QTIDD267 pKa = 3.59 LRR269 pKa = 11.84 EE270 pKa = 3.76 QAISSVRR277 pKa = 11.84 GVDD280 pKa = 3.27 GNLIIAEE287 pKa = 4.23 GTRR290 pKa = 11.84 IEE292 pKa = 4.11 NAISGSGNDD301 pKa = 4.58 LIVANDD307 pKa = 3.9 LQNVLTGGAGNDD319 pKa = 2.54 RR320 pKa = 11.84 FVFHH324 pKa = 7.65 DD325 pKa = 4.16 AASHH329 pKa = 5.68 SANLDD334 pKa = 3.66 TITDD338 pKa = 4.12 FEE340 pKa = 5.59 DD341 pKa = 5.15 GSDD344 pKa = 4.49 LICFVDD350 pKa = 4.99 DD351 pKa = 4.58 DD352 pKa = 4.78 FGPALSFGLLDD363 pKa = 4.56 FSAVGSDD370 pKa = 3.98 VSIQFNDD377 pKa = 4.03 DD378 pKa = 4.05 RR379 pKa = 11.84 ILLEE383 pKa = 4.15 NVSLASLDD391 pKa = 3.73 VNDD394 pKa = 4.49 FLFVV398 pKa = 3.25
Molecular weight: 42.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.677
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.279
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.973
Patrickios 1.367
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|A0A222FBQ6|A0A222FBQ6_9RHOB Alpha/beta hydrolase OS=Labrenzia sp. VG12 OX=2021862 GN=CHH27_25360 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5332
0
5332
1710563
35
4569
320.8
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.351 ± 0.041
0.897 ± 0.011
6.191 ± 0.041
6.17 ± 0.036
4.018 ± 0.024
8.335 ± 0.031
1.979 ± 0.017
5.169 ± 0.026
3.741 ± 0.028
10.37 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.426 ± 0.017
2.836 ± 0.019
4.856 ± 0.024
3.315 ± 0.019
6.246 ± 0.035
5.736 ± 0.024
5.515 ± 0.034
7.274 ± 0.029
1.281 ± 0.014
2.294 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here