Mycoplasma gallopavonis
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 714 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A449B0N6|A0A449B0N6_9MOLU sn-glycerol-3-phosphate transport system permease protein ugpA OS=Mycoplasma gallopavonis OX=76629 GN=ugpA PE=3 SV=1
MM1 pKa = 7.44 AASFDD6 pKa = 3.83 FQHH9 pKa = 7.37 FYY11 pKa = 11.07 LQWKK15 pKa = 6.16 LTKK18 pKa = 10.3 VRR20 pKa = 11.84 VDD22 pKa = 3.59 NVEE25 pKa = 3.32 MDD27 pKa = 3.6 AFVFDD32 pKa = 3.83 SFLDD36 pKa = 3.84 FFEE39 pKa = 5.12 FFAVDD44 pKa = 3.34 YY45 pKa = 10.21 TKK47 pKa = 9.7 FTRR50 pKa = 11.84 VGVVEE55 pKa = 4.14 EE56 pKa = 4.0 QVAYY60 pKa = 10.78 NVDD63 pKa = 3.67 LDD65 pKa = 3.95 YY66 pKa = 11.75 ANQIWFF72 pKa = 3.67
Molecular weight: 8.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.209
IPC2_protein 4.151
IPC_protein 4.05
Toseland 3.846
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.999
Rodwell 3.884
Grimsley 3.77
Solomon 4.037
Lehninger 3.986
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.91
EMBOSS 4.012
Sillero 4.177
Patrickios 3.465
IPC_peptide 4.024
IPC2_peptide 4.151
IPC2.peptide.svr19 4.082
Protein with the highest isoelectric point:
>tr|A0A449AZL8|A0A449AZL8_9MOLU Phosphatidate cytidylyltransferase OS=Mycoplasma gallopavonis OX=76629 GN=cdsA PE=4 SV=1
MM1 pKa = 7.63 ARR3 pKa = 11.84 RR4 pKa = 11.84 DD5 pKa = 3.67 VLTGKK10 pKa = 10.49 GPQSGNKK17 pKa = 9.15 RR18 pKa = 11.84 SHH20 pKa = 6.56 AMNASKK26 pKa = 10.76 RR27 pKa = 11.84 KK28 pKa = 9.92 FNVNLQKK35 pKa = 10.56 VRR37 pKa = 11.84 ITLNGRR43 pKa = 11.84 SQTLRR48 pKa = 11.84 VSAKK52 pKa = 7.55 TLKK55 pKa = 9.16 TLKK58 pKa = 9.91 TKK60 pKa = 10.7 GFII63 pKa = 3.92
Molecular weight: 7.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 10.716
IPC_protein 12.237
Toseland 12.427
ProMoST 12.896
Dawson 12.427
Bjellqvist 12.413
Wikipedia 12.896
Rodwell 12.34
Grimsley 12.471
Solomon 12.91
Lehninger 12.808
Nozaki 12.427
DTASelect 12.413
Thurlkill 12.427
EMBOSS 12.925
Sillero 12.427
Patrickios 12.076
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 9.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
714
0
714
268752
31
3575
376.4
43.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.601 ± 0.099
0.421 ± 0.022
5.318 ± 0.085
6.971 ± 0.095
5.381 ± 0.094
4.644 ± 0.102
1.468 ± 0.034
8.716 ± 0.105
9.835 ± 0.088
10.046 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.041
7.657 ± 0.139
2.736 ± 0.046
4.245 ± 0.077
3.19 ± 0.066
6.431 ± 0.059
5.14 ± 0.059
5.455 ± 0.084
0.983 ± 0.027
4.11 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here