Amino acid dipepetide frequency for Flavobacterium sangjuense

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.261AlaAla: 5.261 ± 0.146
0.714AlaCys: 0.714 ± 0.045
3.48AlaAsp: 3.48 ± 0.062
3.919AlaGlu: 3.919 ± 0.076
3.392AlaPhe: 3.392 ± 0.071
4.945AlaGly: 4.945 ± 0.157
0.967AlaHis: 0.967 ± 0.036
5.504AlaIle: 5.504 ± 0.095
4.883AlaLys: 4.883 ± 0.123
5.992AlaLeu: 5.992 ± 0.109
1.545AlaMet: 1.545 ± 0.05
4.185AlaAsn: 4.185 ± 0.102
2.227AlaPro: 2.227 ± 0.125
2.523AlaGln: 2.523 ± 0.067
1.783AlaArg: 1.783 ± 0.05
4.592AlaSer: 4.592 ± 0.131
5.473AlaThr: 5.473 ± 0.28
4.588AlaVal: 4.588 ± 0.087
0.63AlaTrp: 0.63 ± 0.033
2.278AlaTyr: 2.278 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.611CysAla: 0.611 ± 0.038
0.092CysCys: 0.092 ± 0.011
0.589CysAsp: 0.589 ± 0.05
0.475CysGlu: 0.475 ± 0.028
0.516CysPhe: 0.516 ± 0.031
0.842CysGly: 0.842 ± 0.087
0.204CysHis: 0.204 ± 0.017
0.628CysIle: 0.628 ± 0.024
0.451CysLys: 0.451 ± 0.019
0.707CysLeu: 0.707 ± 0.027
0.137CysMet: 0.137 ± 0.012
0.506CysAsn: 0.506 ± 0.029
0.478CysPro: 0.478 ± 0.045
0.26CysGln: 0.26 ± 0.017
0.203CysArg: 0.203 ± 0.015
0.87CysSer: 0.87 ± 0.058
0.602CysThr: 0.602 ± 0.048
0.495CysVal: 0.495 ± 0.026
0.063CysTrp: 0.063 ± 0.009
0.36CysTyr: 0.36 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.8AspAla: 3.8 ± 0.078
0.494AspCys: 0.494 ± 0.033
2.809AspAsp: 2.809 ± 0.062
3.406AspGlu: 3.406 ± 0.084
3.508AspPhe: 3.508 ± 0.071
3.849AspGly: 3.849 ± 0.09
0.661AspHis: 0.661 ± 0.028
4.084AspIle: 4.084 ± 0.076
4.264AspLys: 4.264 ± 0.123
4.825AspLeu: 4.825 ± 0.086
1.012AspMet: 1.012 ± 0.03
3.128AspAsn: 3.128 ± 0.067
1.341AspPro: 1.341 ± 0.04
1.228AspGln: 1.228 ± 0.038
1.555AspArg: 1.555 ± 0.045
2.981AspSer: 2.981 ± 0.063
2.891AspThr: 2.891 ± 0.089
3.53AspVal: 3.53 ± 0.065
0.714AspTrp: 0.714 ± 0.032
2.814AspTyr: 2.814 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
4.02GluAla: 4.02 ± 0.109
0.396GluCys: 0.396 ± 0.03
2.927GluAsp: 2.927 ± 0.068
4.078GluGlu: 4.078 ± 0.096
2.672GluPhe: 2.672 ± 0.065
3.142GluGly: 3.142 ± 0.078
0.994GluHis: 0.994 ± 0.037
5.167GluIle: 5.167 ± 0.109
5.648GluLys: 5.648 ± 0.14
5.28GluLeu: 5.28 ± 0.111
1.572GluMet: 1.572 ± 0.046
4.363GluAsn: 4.363 ± 0.09
1.384GluPro: 1.384 ± 0.044
2.112GluGln: 2.112 ± 0.062
2.073GluArg: 2.073 ± 0.06
2.972GluSer: 2.972 ± 0.07
3.604GluThr: 3.604 ± 0.088
3.863GluVal: 3.863 ± 0.075
0.541GluTrp: 0.541 ± 0.024
2.085GluTyr: 2.085 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.286PheAla: 3.286 ± 0.062
0.541PheCys: 0.541 ± 0.037
3.428PheAsp: 3.428 ± 0.07
3.289PheGlu: 3.289 ± 0.081
2.772PhePhe: 2.772 ± 0.074
3.745PheGly: 3.745 ± 0.081
0.859PheHis: 0.859 ± 0.034
3.72PheIle: 3.72 ± 0.073
3.376PheLys: 3.376 ± 0.075
4.377PheLeu: 4.377 ± 0.097
1.079PheMet: 1.079 ± 0.04
3.198PheAsn: 3.198 ± 0.07
1.786PhePro: 1.786 ± 0.043
1.588PheGln: 1.588 ± 0.041
1.522PheArg: 1.522 ± 0.043
4.042PheSer: 4.042 ± 0.081
3.66PheThr: 3.66 ± 0.133
3.296PheVal: 3.296 ± 0.066
0.623PheTrp: 0.623 ± 0.03
2.141PheTyr: 2.141 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
4.599GlyAla: 4.599 ± 0.134
0.876GlyCys: 0.876 ± 0.07
3.173GlyAsp: 3.173 ± 0.073
3.13GlyGlu: 3.13 ± 0.066
3.72GlyPhe: 3.72 ± 0.076
5.067GlyGly: 5.067 ± 0.184
1.064GlyHis: 1.064 ± 0.038
5.316GlyIle: 5.316 ± 0.098
4.919GlyLys: 4.919 ± 0.114
5.53GlyLeu: 5.53 ± 0.085
1.553GlyMet: 1.553 ± 0.052
4.271GlyAsn: 4.271 ± 0.13
1.376GlyPro: 1.376 ± 0.045
2.022GlyGln: 2.022 ± 0.055
1.869GlyArg: 1.869 ± 0.05
4.637GlySer: 4.637 ± 0.138
5.657GlyThr: 5.657 ± 0.275
4.324GlyVal: 4.324 ± 0.093
0.719GlyTrp: 0.719 ± 0.03
2.779GlyTyr: 2.779 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
0.887HisAla: 0.887 ± 0.033
0.182HisCys: 0.182 ± 0.016
0.815HisAsp: 0.815 ± 0.033
0.89HisGlu: 0.89 ± 0.036
1.098HisPhe: 1.098 ± 0.043
0.982HisGly: 0.982 ± 0.034
0.438HisHis: 0.438 ± 0.024
1.224HisIle: 1.224 ± 0.038
1.058HisLys: 1.058 ± 0.038
1.576HisLeu: 1.576 ± 0.047
0.298HisMet: 0.298 ± 0.02
0.956HisAsn: 0.956 ± 0.027
0.772HisPro: 0.772 ± 0.032
0.648HisGln: 0.648 ± 0.028
0.561HisArg: 0.561 ± 0.022
0.96HisSer: 0.96 ± 0.031
0.852HisThr: 0.852 ± 0.032
0.865HisVal: 0.865 ± 0.03
0.169HisTrp: 0.169 ± 0.013
0.774HisTyr: 0.774 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.849IleAla: 5.849 ± 0.106
0.796IleCys: 0.796 ± 0.043
4.657IleAsp: 4.657 ± 0.086
4.871IleGlu: 4.871 ± 0.097
3.316IlePhe: 3.316 ± 0.07
5.183IleGly: 5.183 ± 0.094
1.316IleHis: 1.316 ± 0.048
6.096IleIle: 6.096 ± 0.111
5.501IleLys: 5.501 ± 0.111
6.506IleLeu: 6.506 ± 0.129
1.372IleMet: 1.372 ± 0.041
4.759IleAsn: 4.759 ± 0.082
3.275IlePro: 3.275 ± 0.065
2.685IleGln: 2.685 ± 0.061
2.425IleArg: 2.425 ± 0.053
5.508IleSer: 5.508 ± 0.089
5.608IleThr: 5.608 ± 0.145
5.068IleVal: 5.068 ± 0.08
0.701IleTrp: 0.701 ± 0.027
2.884IleTyr: 2.884 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
4.932LysAla: 4.932 ± 0.13
0.407LysCys: 0.407 ± 0.022
4.06LysAsp: 4.06 ± 0.107
5.347LysGlu: 5.347 ± 0.144
2.92LysPhe: 2.92 ± 0.081
4.238LysGly: 4.238 ± 0.095
1.192LysHis: 1.192 ± 0.039
6.313LysIle: 6.313 ± 0.142
6.645LysLys: 6.645 ± 0.154
6.229LysLeu: 6.229 ± 0.136
2.137LysMet: 2.137 ± 0.068
4.964LysAsn: 4.964 ± 0.108
2.53LysPro: 2.53 ± 0.061
2.553LysGln: 2.553 ± 0.07
2.439LysArg: 2.439 ± 0.064
4.435LysSer: 4.435 ± 0.1
4.599LysThr: 4.599 ± 0.091
4.769LysVal: 4.769 ± 0.098
0.746LysTrp: 0.746 ± 0.03
2.859LysTyr: 2.859 ± 0.071
0.0LysXaa: 0.0 ± 0.0
Leu
5.901LeuAla: 5.901 ± 0.094
0.658LeuCys: 0.658 ± 0.024
4.604LeuAsp: 4.604 ± 0.098
5.231LeuGlu: 5.231 ± 0.118
4.776LeuPhe: 4.776 ± 0.106
5.446LeuGly: 5.446 ± 0.089
1.438LeuHis: 1.438 ± 0.043
6.36LeuIle: 6.36 ± 0.118
6.773LeuLys: 6.773 ± 0.147
8.377LeuLeu: 8.377 ± 0.173
1.911LeuMet: 1.911 ± 0.057
5.401LeuAsn: 5.401 ± 0.084
3.586LeuPro: 3.586 ± 0.074
3.008LeuGln: 3.008 ± 0.068
2.714LeuArg: 2.714 ± 0.059
6.362LeuSer: 6.362 ± 0.101
5.603LeuThr: 5.603 ± 0.131
5.211LeuVal: 5.211 ± 0.089
0.726LeuTrp: 0.726 ± 0.032
3.073LeuTyr: 3.073 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
1.446MetAla: 1.446 ± 0.047
0.149MetCys: 0.149 ± 0.013
1.013MetAsp: 1.013 ± 0.037
1.278MetGlu: 1.278 ± 0.041
0.805MetPhe: 0.805 ± 0.033
1.358MetGly: 1.358 ± 0.049
0.39MetHis: 0.39 ± 0.022
1.612MetIle: 1.612 ± 0.042
2.275MetLys: 2.275 ± 0.06
1.804MetLeu: 1.804 ± 0.05
0.611MetMet: 0.611 ± 0.029
1.376MetAsn: 1.376 ± 0.041
0.801MetPro: 0.801 ± 0.035
0.813MetGln: 0.813 ± 0.031
0.742MetArg: 0.742 ± 0.032
1.438MetSer: 1.438 ± 0.042
1.255MetThr: 1.255 ± 0.037
1.315MetVal: 1.315 ± 0.037
0.145MetTrp: 0.145 ± 0.013
0.679MetTyr: 0.679 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
4.374AsnAla: 4.374 ± 0.091
0.629AsnCys: 0.629 ± 0.046
3.385AsnAsp: 3.385 ± 0.072
3.356AsnGlu: 3.356 ± 0.07
3.35AsnPhe: 3.35 ± 0.062
4.723AsnGly: 4.723 ± 0.147
1.071AsnHis: 1.071 ± 0.039
4.616AsnIle: 4.616 ± 0.086
4.088AsnLys: 4.088 ± 0.096
5.434AsnLeu: 5.434 ± 0.084
1.188AsnMet: 1.188 ± 0.033
4.138AsnAsn: 4.138 ± 0.094
3.337AsnPro: 3.337 ± 0.106
2.386AsnGln: 2.386 ± 0.061
1.865AsnArg: 1.865 ± 0.049
4.232AsnSer: 4.232 ± 0.085
4.063AsnThr: 4.063 ± 0.123
4.008AsnVal: 4.008 ± 0.097
0.812AsnTrp: 0.812 ± 0.029
3.017AsnTyr: 3.017 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
2.546ProAla: 2.546 ± 0.117
0.307ProCys: 0.307 ± 0.038
1.811ProAsp: 1.811 ± 0.046
2.413ProGlu: 2.413 ± 0.065
1.993ProPhe: 1.993 ± 0.06
2.086ProGly: 2.086 ± 0.07
0.499ProHis: 0.499 ± 0.025
2.85ProIle: 2.85 ± 0.057
2.534ProLys: 2.534 ± 0.068
2.78ProLeu: 2.78 ± 0.061
0.731ProMet: 0.731 ± 0.028
2.603ProAsn: 2.603 ± 0.065
0.913ProPro: 0.913 ± 0.068
1.041ProGln: 1.041 ± 0.035
0.834ProArg: 0.834 ± 0.034
2.488ProSer: 2.488 ± 0.094
2.824ProThr: 2.824 ± 0.136
2.508ProVal: 2.508 ± 0.079
0.274ProTrp: 0.274 ± 0.02
1.381ProTyr: 1.381 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.062GlnAla: 2.062 ± 0.052
0.201GlnCys: 0.201 ± 0.016
1.512GlnAsp: 1.512 ± 0.039
1.995GlnGlu: 1.995 ± 0.056
1.728GlnPhe: 1.728 ± 0.043
1.73GlnGly: 1.73 ± 0.048
0.517GlnHis: 0.517 ± 0.024
2.656GlnIle: 2.656 ± 0.054
2.972GlnLys: 2.972 ± 0.079
3.31GlnLeu: 3.31 ± 0.075
0.764GlnMet: 0.764 ± 0.03
2.41GlnAsn: 2.41 ± 0.053
1.104GlnPro: 1.104 ± 0.036
1.295GlnGln: 1.295 ± 0.05
1.016GlnArg: 1.016 ± 0.034
1.931GlnSer: 1.931 ± 0.043
2.28GlnThr: 2.28 ± 0.053
1.969GlnVal: 1.969 ± 0.058
0.342GlnTrp: 0.342 ± 0.019
1.375GlnTyr: 1.375 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
1.837ArgAla: 1.837 ± 0.052
0.229ArgCys: 0.229 ± 0.016
1.506ArgAsp: 1.506 ± 0.047
1.924ArgGlu: 1.924 ± 0.056
1.732ArgPhe: 1.732 ± 0.049
1.612ArgGly: 1.612 ± 0.051
0.523ArgHis: 0.523 ± 0.026
2.591ArgIle: 2.591 ± 0.059
2.391ArgLys: 2.391 ± 0.073
2.745ArgLeu: 2.745 ± 0.069
0.877ArgMet: 0.877 ± 0.033
1.875ArgAsn: 1.875 ± 0.057
0.947ArgPro: 0.947 ± 0.028
0.929ArgGln: 0.929 ± 0.031
1.052ArgArg: 1.052 ± 0.042
1.562ArgSer: 1.562 ± 0.051
1.692ArgThr: 1.692 ± 0.047
1.97ArgVal: 1.97 ± 0.052
0.32ArgTrp: 0.32 ± 0.019
1.335ArgTyr: 1.335 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
4.376SerAla: 4.376 ± 0.123
0.701SerCys: 0.701 ± 0.032
3.46SerAsp: 3.46 ± 0.063
3.5SerGlu: 3.5 ± 0.069
3.923SerPhe: 3.923 ± 0.077
5.21SerGly: 5.21 ± 0.145
1.06SerHis: 1.06 ± 0.035
5.027SerIle: 5.027 ± 0.074
4.589SerLys: 4.589 ± 0.099
5.893SerLeu: 5.893 ± 0.086
1.22SerMet: 1.22 ± 0.033
4.264SerAsn: 4.264 ± 0.112
2.512SerPro: 2.512 ± 0.078
2.273SerGln: 2.273 ± 0.059
1.774SerArg: 1.774 ± 0.045
4.808SerSer: 4.808 ± 0.141
4.43SerThr: 4.43 ± 0.178
4.398SerVal: 4.398 ± 0.102
0.699SerTrp: 0.699 ± 0.03
2.777SerTyr: 2.777 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
5.371ThrAla: 5.371 ± 0.261
0.618ThrCys: 0.618 ± 0.058
3.398ThrAsp: 3.398 ± 0.09
3.282ThrGlu: 3.282 ± 0.07
3.607ThrPhe: 3.607 ± 0.12
5.35ThrGly: 5.35 ± 0.252
0.915ThrHis: 0.915 ± 0.029
6.098ThrIle: 6.098 ± 0.193
3.914ThrLys: 3.914 ± 0.082
5.764ThrLeu: 5.764 ± 0.101
1.053ThrMet: 1.053 ± 0.038
4.268ThrAsn: 4.268 ± 0.148
3.124ThrPro: 3.124 ± 0.146
2.104ThrGln: 2.104 ± 0.057
1.558ThrArg: 1.558 ± 0.041
4.764ThrSer: 4.764 ± 0.187
5.549ThrThr: 5.549 ± 0.299
5.19ThrVal: 5.19 ± 0.219
0.762ThrTrp: 0.762 ± 0.061
2.832ThrTyr: 2.832 ± 0.127
0.0ThrXaa: 0.0 ± 0.0
Val
4.755ValAla: 4.755 ± 0.105
0.651ValCys: 0.651 ± 0.046
3.512ValAsp: 3.512 ± 0.072
3.752ValGlu: 3.752 ± 0.085
3.461ValPhe: 3.461 ± 0.068
4.014ValGly: 4.014 ± 0.092
0.923ValHis: 0.923 ± 0.036
5.085ValIle: 5.085 ± 0.085
4.456ValLys: 4.456 ± 0.089
5.595ValLeu: 5.595 ± 0.095
1.258ValMet: 1.258 ± 0.042
4.026ValAsn: 4.026 ± 0.101
2.292ValPro: 2.292 ± 0.067
1.766ValGln: 1.766 ± 0.044
1.891ValArg: 1.891 ± 0.051
4.834ValSer: 4.834 ± 0.094
4.95ValThr: 4.95 ± 0.191
4.549ValVal: 4.549 ± 0.098
0.597ValTrp: 0.597 ± 0.025
2.529ValTyr: 2.529 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.606TrpAla: 0.606 ± 0.023
0.101TrpCys: 0.101 ± 0.01
0.55TrpAsp: 0.55 ± 0.029
0.606TrpGlu: 0.606 ± 0.023
0.545TrpPhe: 0.545 ± 0.026
0.563TrpGly: 0.563 ± 0.03
0.194TrpHis: 0.194 ± 0.013
0.722TrpIle: 0.722 ± 0.027
0.763TrpLys: 0.763 ± 0.029
0.884TrpLeu: 0.884 ± 0.038
0.285TrpMet: 0.285 ± 0.017
0.736TrpAsn: 0.736 ± 0.031
0.185TrpPro: 0.185 ± 0.014
0.418TrpGln: 0.418 ± 0.023
0.327TrpArg: 0.327 ± 0.019
0.689TrpSer: 0.689 ± 0.034
0.805TrpThr: 0.805 ± 0.061
0.626TrpVal: 0.626 ± 0.024
0.14TrpTrp: 0.14 ± 0.014
0.449TrpTyr: 0.449 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.363TyrAla: 2.363 ± 0.053
0.348TyrCys: 0.348 ± 0.02
2.203TyrAsp: 2.203 ± 0.048
2.15TyrGlu: 2.15 ± 0.063
2.496TyrPhe: 2.496 ± 0.068
2.516TyrGly: 2.516 ± 0.063
0.782TyrHis: 0.782 ± 0.03
2.706TyrIle: 2.706 ± 0.06
2.811TyrLys: 2.811 ± 0.071
3.549TyrLeu: 3.549 ± 0.067
0.7TyrMet: 0.7 ± 0.027
2.644TyrAsn: 2.644 ± 0.057
1.448TyrPro: 1.448 ± 0.043
1.516TyrGln: 1.516 ± 0.05
1.445TyrArg: 1.445 ± 0.043
2.789TyrSer: 2.789 ± 0.075
3.125TyrThr: 3.125 ± 0.16
2.355TyrVal: 2.355 ± 0.058
0.473TyrTrp: 0.473 ± 0.023
1.925TyrTyr: 1.925 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2637 proteins (965529 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski