Streptomyces sp. IB201691-2A2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A550ICH9|A0A550ICH9_9ACTN Deoxyribodipyrimidine photo-lyase OS=Streptomyces sp. IB201691-2A2 OX=2561920 GN=E4K73_07680 PE=3 SV=1
MM1 pKa = 7.64SSSCCSTDD9 pKa = 3.57GSCSTATTDD18 pKa = 3.37RR19 pKa = 11.84ATVTEE24 pKa = 4.68GVAVTTTYY32 pKa = 10.97AVSGMTCGRR41 pKa = 11.84CKK43 pKa = 10.19ATLTEE48 pKa = 4.69VIGEE52 pKa = 4.16LDD54 pKa = 3.47GVTGVDD60 pKa = 2.88VDD62 pKa = 4.31LATGRR67 pKa = 11.84VTVVATAEE75 pKa = 4.24PDD77 pKa = 3.28DD78 pKa = 3.85TLIAEE83 pKa = 4.57VVDD86 pKa = 3.81DD87 pKa = 5.36AGYY90 pKa = 10.85EE91 pKa = 4.04LTGRR95 pKa = 11.84AA96 pKa = 3.89

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A550HNF2|A0A550HNF2_9ACTN NAD(P)-dependent oxidoreductase OS=Streptomyces sp. IB201691-2A2 OX=2561920 GN=E4K73_31755 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9596

0

9596

3162214

23

6369

329.5

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.114 ± 0.036

0.755 ± 0.007

6.044 ± 0.018

5.719 ± 0.023

2.764 ± 0.014

9.446 ± 0.029

2.272 ± 0.011

3.253 ± 0.017

2.303 ± 0.022

10.252 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.01

1.887 ± 0.014

5.993 ± 0.023

2.812 ± 0.014

7.847 ± 0.025

5.359 ± 0.019

6.273 ± 0.019

8.447 ± 0.023

1.561 ± 0.012

2.136 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski